Regeneración de cartílago mediante el uso de células madre de pulpa dental humanas (hDPSCs)

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Introduction: cartilage tissue is a fundamental tissue, since it provides support to our body. When the articular cartilage is injured it undergoes complex and limited regeneration. For this reason, it is important to look for alternatives and expand the solutions that are currently being used to treat diseases in which the alteration of the articular cartilage occurs, such as osteoarthritis. Based on this idea, tissue engineering has recently developed different tools to try to regenerate cartilage when regeneration by other means is not possible. Among the existing strategies, the use of hydrogels combined with chondrocytes and / or stem cells of different origins has been promoted. Objective: our objective in this work was to design a construct based on a hydrogel, then alginate and later an alginate-agarose mixture, combined with chondrocytes or human dental pulp stem cells (hDPSCs), with chondrogenic induction capacity. Material and methods: primary chondrocytes and stem cells from human dental pulp were isolated and characterized. A culture system was developed to favor chondral differentiation, first in two dimensions and then in three dimensions. Alginate hydrogels and alginate-agarose copolymers were biologically and biomechanically characterized and manufactured. The culture of chondrocytes and hDPSCs on said supports was optimized and their regenerative capacity was evaluated both in an animal model and in vitro. Conclusion: the results send indicate the chondrogenic potential of hDPSCs as an alternative to primary chondrocytes for chondral regeneration. Likewise, we propose a construct based on an alginate-agarose copolymer with suitable biomechanical properties to be used in joint cartilage regeneration models.
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