Journal of Clinical and Experimental Dentistry. 2020. Vol. 12, no. 7

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    Condylar form alteration on skeletal class II patients that underwent orthognathic surgery : an overview of systematic reviews
    (2020) Francisco, Inês; Guimarães, Adriana; Lopes, Margarida; Lucas, António; Caramelo, Francisco; do Vale, Francisco-José-Fernandes
    Bilateral sagittal split osteotomy (BSSO) is commonly considered as the surgical technique of election for the treatment of skeletal class II with mandibular hypoplasia. After orthognathic surgery, condylar resorption can occur as a surgical relapse, which may affect the temporomandibular joint. Objective: This study aimed to summarise published systematic review that assess if orthognathic surgery with mandibular advancement performed on skeletal class II patients results in condylar form alteration. A literature search was performed using the electronic databases PubMed, Web of Science, Cochrane Library, Embase along with several sources of grey literature. Selection Criteria: Inclusion criteria were systematic reviews published until December 2019, of skeletal class II patients aged more than 18 years old that underwent BSSO with mandibular advancement surgery. Data collection: The electronic search identified 37 publications. Four publications fulfilled the inclusion criteria and were included in this meta-analysis. Qualitative assessment of the selected studies was performed using the Assessment of Multiple Systematic Reviews ? AMSTAR 2 checklist. Four systematic reviews were included in this review. Despite its low incidence all studies reported condylar resorption. However, there were methodological limitations in all assessed articles. The alteration of the condylar form may be a consequence of BSSO with mandibular advancement surgery. Additional high quality prospective research assisted by 3D-imaging technology is needed to allow more definite conclusions.
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    The effect of composite resin preheating on marginal adaptation of class II restorations
    (2020) Darabi, Farideh; Tayefeh-Davalloo, Reza; Tavangar, Seyedeh-Maryam; Naser Alavi, Fereshteh; Boorboo-Shirazi, Mozhdeh
    One of the problems with a high filler content composite resins is gap formation at restorative material?tooth interface. The present study investigated the effect of preheating composite resins on the formation of marginal gap in Cl II restorations. In this in vitro study Sixty Cl II cavities were prepared on the mesial and distal surfaces of 30 extracted premolar teeth. The gingival floor of cavities was placed 1 m below the CEJ. The samples were randomly allocated to 4 groups for restoration placement: group 1, Filtek P60 composite resin at room temperature; group 2, Filtek P60 composite resin at 68°C; group 3, X-tra fil composite resin at room temperature; and group 4, X-tra fil composite resin at 68°C. After a thermocycling procedure, the teeth were sectioned longitudinally in a buccolingual direction. Then the marginal gaps of the samples were measured at proximal and gingival margins under a scanning electron microscope at ×2000 magnification in µm. The data were analyzed with SPSS 21, using one-way ANOVA, post hoc Tukey tests and paired t-test (?=0.05). Groups 2 and 4 exhibited significantly lower marginal gaps, compared to groups 1 and 3, at both enamel (P<0.0001 and P=0.001, respectively) and dentinal walls (P<0.0001). In all the groups, there was significantly less marginal gaps at composite-enamel wall compared to composite-dentin wall interfaces (P<0.0001). There was no significant difference between groups 1 and 3 and groups 2 and 4 in enamel walls (p= 0.96, p= 0.99 respectively) and dentinal walls (p= 0.85, p=0.98 respectively). Preheating resulted in a decrease in marginal gaps in both composite resins. The effect of composite resin type on marginal adaptation was the same.
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    Effect of different polishing techniques on surface roughness and bacterial adhesion of three glass ionomer-based restorative materials : in vitro study
    (2020) Ismail, Hoda S.; Ali, Ashraf I.; Abo El-Ella, Mohammed A.; Mahmoud, Salah-Hassab
    Although many reports concluded that polishing of glass ionomers is crucial for smoother surface and limiting the adhesion of cariogenic bacteria, there is no specific surface treatment protocol recommended. A novel material in the same category was released recently claimed to have surface smoothness comparable to resin composite and bacterial adhesion less than other types of glass ionomers. In this study, different polishing systems were tested with three glass ionomers one of them is the novel material to find the most appropriate polishing protocol. Objectives: To evaluate and compare the surface roughness and bacterial adhesion to resin modified glass ionomer, bioactive ionic resin and conventional glass ionomer restorative materials after different polishing protocols in vitro. The materials tested includes resin modified glass ionomer, bioactive ionic resin, and conventional glass ionomer. The polishing protocols were divided into four groups: group 1 = (Mylar matrix strips, Control), group 2 = (one-step, PoGo), group 3 = (two-step, Prisma Gloss) and group 4 = (three-step, Sof-Lex). From each material, eleven cylindrical specimens were prepared for each group according to the manufacturers? instructions. The surface roughness for all specimens was measured using atomic force microscope in tapping mode. the same specimens were subjected to bacterial adhesion testing after being coated with artificial saliva. Data were analyzed with two-way analysis of variance followed by Post hoc multiple comparisons. The highest Ra and S. mutans adhesion values were recorded for all materials in two-step group. The lowest Ra and S. mutans adhesion values were seen in one-step and three step groups. One-step polishing system was more effective and may be preferable for polishing of the three studied glass ionomer-based materials compared to two-step and three-step systems.
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    Effect of in situ aspartame mouthwash to prevent intrinsic and extrinsic erosive tooth wear
    (2020) Ionta, Franciny-Querobim; Bassoto, Marcela-de Azevedo-Garcia; dos Santos, Natália-Mello; Di Campli, Fabiana; Honório, Heitor-Marques; da Silva, Thiago-Cruvinel; Buzalaf, Marilia-Afonso-Rabelo; Rios, Daniela
    The aim was to evaluate whether aspartame regular mouthwash prior to erosive challenges with citric or hydrochloric acids would be able to prevent erosive enamel wear. This randomized, single blind in situ study was conducted with 3 crossover phases of 5 days. Polished bovine enamel blocks (n=252) were randomly divided among 6 groups/ 3 phases/ 21 volunteers. The groups under study were: aspartame solution (0.024% of aspartame in deionized water - experimental group), deionized water (negative-control) and stannous-containing solution (Elmex® Erosion Protection Dental Rinse; positive-control); subjected to erosion on citric acid or hydrochloric acid. Four times per day the volunteers rinsed the intraoral appliance with the respective solutions (in situ) prior to immersion of half of the appliance in 0.05M citric acid and the other half in 0.01M hydrochloric acid for 120 seconds (extraoral). The response variable was enamel loss by profilometry. Data were analyzed by ANOVA and Tukey?s test (p<0.05). No difference on enamel loss was found between aspartame solution and deionized water. Stannous-solution resulted in less enamel loss compared to deionized water. Hydrochloric acid resulted in higher enamel loss than citric acid. In this model, aspartame was not able to prevent erosive tooth wear against citric or hydrochloric acids.
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    The bleaching efficacy of carbamide peroxide gels containing potassium nitrate desensitizer
    (2020) Costacurta, Adriana-Osten; Borges, Carolina-Elisa-Pereira; Centenaro, Camila; Correr, Gisele Maria; Kaizer, Marina-da Rosa; Gonzaga, Carla Castiglia
    To evaluate the bleaching efficacy of at-home carbamide peroxide (CP) gels in two concentrations, containing or not a desensitizing agent. Forty incisors were divided into four groups (n=10), according to gel concentrations (10% or 22%), and presence or not of 3% potassium nitrate in the gel. A thin layer of gel was applied to the buccal surface of each tooth for 2h/day for 4 weeks. Bleaching efficacy was measured using a spectrophotometer, and ?E*ab, ?E00 and ?WID were calculated. Measurements were performed at baseline, 7, 14, 21, 28, and 35 days following the first gel application. Data were analyzed by two-way RM-ANOVA and Tukey?s test (?=0.05). Regarding gel concentration or potassium nitrate inclusion, both gels resulted in color change above the perceptibility thresholds, which were similar between gels. Regarding time, significant differences were observed between color change values at 7 days and other time periods. ?WID ranged from 3.8 to 9.6. Significant moderate to strong positive correlation was observed among the parameters. Nor the CP concentration, neither the inclusion of potassium nitrate in the gel, had influence on bleaching efficacy. All gels were effective and showed good results from the first weeks? application.
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    Survey on awareness and preference of ceramic bracket debonding techniques among orthodontists
    (2020) Ngan, Aileen Y.; Bollu, Prashanti; Chaudhry, Kishore; Stevens, Richard; Subramani, Karthikeyan
    The objectives of this study was to evaluate the awareness of different ceramic bracket debonding techniques among orthodontists in the USA and the most commonly used debonding technique for ceramic bracket removal. A survey on preference for debonding and awareness of debonding techniques was emailed to 2,227 members of the American Association of Orthodontists (AAO). 119 orthodontists completed the survey. 111 responses were included in the study analysis of ceramic bracket users. The most common technique used was mechanical debonding. 86.5% used a specially designed bracket removing plier from the manufacturer. Overall, there were 59.5% of surveyed orthodontists who were aware of electrothermal debonding, 73% were unaware of ultrasonic debonding and 83.8% were unaware of laser debonding. There were more orthodontists with an affiliation with an academic institution aware of electrothermal debonding (p=0.002). There also was a trend of orthodontists having no affiliation with an institution who were unaware of laser debonding (p=0.015). This survey showed that the majority of orthodontists who responded to the questionnaire were unaware of alternative debonding techniques of ceramic brackets. All orthodontists who use ceramic brackets utilized mechanical debonding technique.
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    Osteonecrosis of the jaws associated with denosumab : study of clinical and radiographic characteristics in a series of clinical cases
    (2020) Tofé, Victoria I.; Bagán Debón, Leticia; Bagán Sebastián, José Vicente
    The objective of this study was to describe the clinical and radiographic characteristics of our series of medication-related osteonecrosis of the jaws (ONJ) associated with denosumab. We presented 15 cases of ONJ associated with denosumab; 11 received treatment for their osteoporosis and four for cancer treatments. We recorded the most frequent clinical findings, symptoms and radiographic characteristics in our patient group, as well as local and systemic contributing factors. The mean time of treatment with denosumab was 23.83 ± 12.84 months. 40% of the patients had a previous history of treatment with bisphosphonates. The most common local factor was tooth extraction (11 cases; 73.3%), and in most cases there was necrotic bone exposure (13/15, 86.67%). Osteolysis, bone sclerosis and cortical erosion were the most common radiographic findings. Stage 1 was the most frequent, present in 60% of the cases. In our patient group, most were in the early stages of ONJ.
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    Biochemical and microbiological analysis of the saliva of institutionalized elderly : with edentulism, use of dentures and presence of biofilm
    (2020) Montenegro, Luiza A. S.; Silva e Farias, Ilky-Pollansky; de Araújo, Elza-Cristina-Farias; de Pontes, Jannerson-César-Xavier; Raymundo, Maria-Letícia-Barbosa; de Sousa, Silmone-Alves; de Almeida, Leopoldina de Fátima Dantas; Cavalcanti, Yuri-Wanderley
    To analyze biochemical and microbiological parameters of the saliva of institutionalized elders and to investigate the relation of these parameters with edentulism, use of dentures and presence of biofilm. A cross-sectional study carried was out in seven long-term institutions. Samples (n=161) of unstimulated saliva were collected for analysis of salivary flow, quantification of total proteins and identification of microorganisms. Oral examination was carried out to verify the number of missing teeth, the use of dentures and the presence of visible biofilm on the surface of teeth and dentures. Associations were performed using chi-square or Fisher?s exact test (?<0.05). Mann-Whitney Test was used to identify differences in the salivary flow and total proteins (?<0.05). There was no association between the type of edentulism and use of dentures with biochemical and microbiological parameters of saliva (p>0.05). Associations were observed between the presence of dentures biofilm and the colonization of Streptococcus sp. (p=0.038) and Candida sp. (p=0.03). The absence of teeth and use of dentures do not influence the amount of total proteins and the microorganisms count in saliva. Denture biofilms are associated with the presence of Streptococcus sp. and Candida sp. in saliva of institutionalized elders.
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    Effect of Chlorhexidine on durability of two self-etch adhesive systems
    (2020) Kazemi-Yazdi, Haleh; Saeed-Nezhad, Mahdieh; Rezaei, Sadaf
    Despite of the rapid development in the field of dental adhesives, the issue of reduction in dentin bond durability has still not been resolved. The activity of dentinal endogenous enzymes such as MMPs is one of the most important causes of failure in resin composite restorations. The aim of this study was to investigate the influence of Chlorhexidine on micro-tensile bond strength of two types of commercially available self-etch adhesives. Twenty four sound and freshly extracted molars were selected. Four standardized flat mid-coronal dentinal disks were prepared from each tooth. The specimens were randomly assigned to 6 groups (n=16). Groups A(control group) and B were treated with Clearfill SE Bond based on the manufacturer?s instructions. Groups C and D were treated with 2% Chlorhexidine 60 seconds before applying Clearfill SE Bond. Groups E and F were treated with Peak Universal Bond according to the manufacturer?s instructions. All groups were stored in distilled water in room temperature. Microtensile bond strength in groups A, C, and E were tested 24 hours after preparation, while microtensile bond strength in groups B, D, and F were tested after 3 months storage and 3000 thermal cycles(5-55 °C). Statistical analysis was performed with SPSS 20 and µTBS test results were analyzed using the Two-way ANOVA test. µTBS was not significantly different between groups A, C, and E after 24 hours (P>0.5). There was no significant difference between groups B (Clearfill SE Bond + Aging) and D (Clearfill SE Bond + 2% CHX + Aging). The Peak Universal µTBS significantly decreased after the aging procedure (P<0.001). Based on the findings of this study, pretreatment with 2% CHX had no negative effect on the Clearfill SE Bond µTBS. However the µTBS of 0.2% CHX contained Peak Universal adhesive decreased significantly after aging.
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    Red propolis hydroalcoholic extract inhibits the formation of Candida albicans biofilms on denture surface
    (2020) Leite, Karla-Lorene-de França; Martins, Mariana-Leonel; de Medeiros, Mariana-Marinho-Davino; Bezerra, Natanael-Victor-Furtunato; Brito, Camila-Santos-de Mattos; de Almeida, Leopoldina de Fátima Dantas; Cavalcanti, Yuri-Wanderley
    To evaluate the antifungal activity of the red propolis hydroalcoholic extract (RPHE) against Candida albicans biofilms. The minimum inhibitory and fungicidal concentrations (MIC and MFC) of the RPHE were determined by the microdilution technique. C. albicans biofilms were formed on the surface of resin specimens preconditioned with artificial saliva (1h). The specimens (N=48) were equally divided according to the four solutions used for anti-biofilm evaluation (n=12 per group). After overnight incubation, biofilms were daily exposed (2x/day for 15 min) along 3 days with 3% RPHE, 0.12% chlorhexidine (CHX), 50,000 IU/mL nystatin (NYS) or saline (0.9% NaCl). Biofilms were analyzed regarding the number of viable microorganisms (CFU/mL), the metabolic activity (MTT assay) and the proportion of hyphae (optical microscopy). The MIC and MFC of RPHE were respectively 0.29 mg/mL (0.03%) and 1.17 mg/mL (0.12%). There was no difference in the microorganisms? viability (CFU/mL) among groups treated with RPHE (4.92×103), CHX (3.33×102) or NYS (6.8×104), being all of them different from NaCl (3.93×107) (p<0.05). The CHX (0.133) had the lowest metabolic activity (p<0.05), followed by RPHE (0.292) and NYS (0.302) (p>0.05). All experimental groups had a mean proportion of hyphae <10%, lower than NaCl (70%). RPHE has antifungal activity against C. albicans biofilms, suggesting its use for the biofilm control on denture surfaces.
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    UC-USP collaborative exercise on photobiomodulation therapy in neurological orofacial disturbances
    (2020) Nunes, Tiago; Caetano, Catarina; Pimenta, Miguel; Saraiva, José; Rocha, Salomâo; Freitas, Patrícia; Figueiredo, José; Pereira, Sónia-Margarida-Alves; Corte-Real, Ana
    Neurosensory peripheral disorders are one of the most common risks associated with iatrogenic and/or post-traumatic injuries. It is often related to disability. Photobiomodulation therapy (PBMT) is a nonsurgical and safe procedure which can accelerate and improve the regeneration of injured biological tissue. This study aims to analyze the impact of PBMT, in the quality of life and impairment of individuals with orofacial neurological peripheral disturbance. A retrospective analysis in the database of the dental traumatology clinic of the Hospital Centre of the University of Coimbra/Faculty of Medicine of the University of Coimbra was performed.5 out of 50 individuals were selected, according to the selection criteria. The neurosensory activity was assessed by a pinprick nociceptive test and the EQ-5D-5L self-report questionnaire was used to analyse the quality of life. The study was performed in two phases:1) inactive laser or placebo phase, for one month and 2) active laser or treatment phase. A diode low-level laser device (SIROLaserBlue;Sirona,Germany) was used, according to our protocol. A collaborative protocol in the PBMT influence in individuals with neurosensory peripheral disturbances was studied. There was no improvement in the neurosensory activity nor in the quality of life, in the placebo phase. After the treatment phase, the EQ-5D-5L final results reported no problems in all of the five dimensions, except for anxiety/depression in individuals with long-standing neurosensory peripheral disturbances. The EQ-VAS scores increased in all the individuals. Our results supported the improvement of quality of life and impairment reduction in the individuals submitted to PBMT.
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    Role of cone-beam computed tomography (CBCT) in diagnosis and treatment planning of two-rooted maxillary lateral incisor with palatogingival groove. Case report
    (2020) Giner-Lluesma, Teresa; Micó Muñoz, Pedro; Prada, Ilaria; Micó Martínez, Pablo; Collado Castellanos, Nicolás; Manzano, Alberto; Albero, Alberto
    The embryonic root groove is an anatomical abnormality that starts in the cingulum and extends longitudinally down the long axis root towards the apex. This developmental anomaly is more frequently reported in maxillary lateral incisors. Gu YC in 2011 established three types of radicular grooves depending on its severity. According to this classification, type III presents a greater diagnostic and therapeutic complexity. The prevalence of palatogingival grooves in maxillary lateral incisors ranges from 1.9 to 14%. This case report provides valuable information about the diagnosis and treatment plan of palatogingival grooves with Cone-beam computed tomography (CBCT) scan. The patient was referred to the University Dental Clinic of European University of Valencia, with recurrent abscesses at the upper right lateral incisor region for the last two years. Palpation and percussion tests were positive for tooth 1.2. There was no clinical history of caries or previous trauma. Periapical radiography showed periapical radiolucent lesions located, not only in the apical area of tooth 1.2, but also in tooth 1.3. Both teeth had previously been endodontically treated. Periodontal probing showed normal values. CBCT scan was perfomed in order to establish a definitive diagnosis and appropriate treatment plan. The complex anatomy of the palatal root groove requires detailed knowledge of the internal root morphology for endodontic treatment success. This complementary tool allows a more accurate image of hard tissue structures, such as palatal grooves and/or accessory roots, in comparison to conventional periapical radiography. The treatment plan of this primary periodontal lesion with secondary endodontic involvement was as follows: periapical surgery combined with root amputation and sealing with MTA, and guided bone regeneration.
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    Compositional and microhardness findings in tooth affected by X-linked hypophosphatemic rickets
    (2020) Carvalho, Francisco-Samuel-Rodrigues; Feitosa, Victor-Pinheiro; Sá Roriz Fonteles, Cristiane; Rodrigues-Ribeiro, Thyciana; Araújo, Bruno-Sousa; Ayala, Alejandro-Pedro; Gurgel Costa, Fábio Wildson
    This study aimed to evaluate the X-linked hypophosphatemic rickets (XLHR)-related compositional and microhardness tooth aspects. One affected and one non-affected teeth by XLHR were sectioned transversely, and each section was separated for Micro-Raman spectroscopy, Knoop microhardness and scanning electron microscopy with energy dispersive x-ray microanalysis (SEM-EDS). The outcomes of these analyses were assessed. Outcomes of Raman analysis of inorganic/organic components (~958/~1250+~1450 cm?1) and carbonate/phosphate (~1070/~958 cm?1) ratios showed areas of altered enamel and dentin (interglobular dentin, calcospherites, and mantle dentin) with an increase of inorganic content in the rickets tooth. Microhardness reduction was observed in the affected tooth, with a more evident drop in regions of mantle dentin, interglobular dentin, and calcospherites. SEM-EDS analysis showed demonstrated the absence of calcium and phosphorus in interglobular spaces. In conclusion, compositional and structural deficiencies were observed in deciduous tooth affected by XLHR. Also, it was observed the absence of hydroxyapatite in the interglobular dentin by using Raman spectroscopy analysis.
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    Effect of bleaching agents on hardness, surface roughness and color parameters of dental enamel
    (2020) de Carvalho, Ana-Caroline-Godoy; de Souza, Terezinha-Fatima; Liporoni, Priscila-Christiane-Suzy; Pizi, Eliane-Cristina-Gava; Matuda, Larissa-Sgarbosa-de Araújo; Catelan, Anderson
    In this study was evaluated the effect of carbamide peroxide (CP) and hydrogen peroxide (HP) in different concentrations on hardness, roughness, and color parameters (color change - ?E, lightness - ?L, and yellow-blue axis - ?b) of bovine teeth. Fifty square dental blocks (7 x 7 x 2 mm) were submitted to initial readings of Knoop hardness, surface roughness (Ra), and color parameters. Specimens were divided into 5 groups (n = 10): control group was kept in artificial saliva during the experimental period; CP 20% was bleached for 2 h daily for 14 days, HP 9.5% was bleached for 30 min daily for 14 days, HP 38% the bleaching gel was applied for 15 min, gel was removed and it was reapplied for two more times, the bleaching session was repeated for another 2 times every 7 days, totaling three sessions, and in CP 45% three sessions of 30 min each were performed of 7 in 7 days. All groups after the bleaching procedures had the bleaching gel removed, washed, and kept in artificial saliva. At the end of bleaching treatment, the hardness, roughness, and color parameters (?E, ?L, and ?b) were re-evaluated. Data were analyzed by ANOVA, Tukey, and Dunnett tests (? = 0.05). Bleaching promoted a reduction in hardness, the CP 45% showed the lowest hardness and the CP 20% the highest, the HP 9.5% and HP 38% showed intermediate values of hardness. Bleaching agents did not affect the roughness. CP 20% and HP 38% promoted the highest values of ?E and higher reduction of yellowish tone of tooth. Lightness increase after bleaching treatment for all groups. All the bleaching agents tested showed effectiveness, but with reduced hardness.