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Language Journal of Clinical and Experimental Dentistry. 2020. Vol. 12, no. 5
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- Effect of framework type on survival probability of implant-supported temporary crowns : an in vitro study(2020) Rodrigues, Vinicius-Aneas; Dal Piva, Amanda-Maria-de Oliveira; Yamaguchi, Claudio-Akira; Borges, Alexandre- Luiz-Souto; Mukai, Marcio-Katsuyoshi; Tribst, João Paulo MendesThis in vitro study evaluated the effect of framework type on the survival probability of temporary implant-supported crowns and on the implant platform structure after dynamic fatigue. Thirty (30) external hexagon implants (3.75 x 10 mm) were embedded in acrylic resin following the ISO-14801. Standardized temporary crowns (n=10, N=30) were manufactured in acrylic resin and divided according to the framework type: Total plastic, Plastic with CoCr base and Titanium. The crowns were installed onto the implants (20N.cm) and fatigued (100N, 2 Hz) to determine the crowns? survival probability for missions of 300.000 and 600.000 cycles. Fatigue data were submitted to the Kaplan-Meier test followed by Wilcoxon and Log Rank, all with ? = 5%. The implant platforms were parametrically inspected based on the scanning before and after the fatigue to evaluate the damage. The strain values were analyzed using One-way ANOVA and Tukey test, all with ? = 5%. ANOVA revealed that the Total plastic showed less implant damage (-0.07 ± -0.03 mm) than the Plastic with CoCr base (-0.08 ± -0.04 mm) and the Titanium (-0.10 ± -0.01 mm) frameworks. Therefore, the framework type to manufacture implant-supported temporary crowns influences the fatigue survival of the restoration and the implant platform damage. The Plastic with CoCr base and Titanium frameworks showed superior reliability than the Total plastic framework which could not survive 600,000 cycles. The Plastic with CoCr base and the Titanium framework are suitable for restorations over 3 months in use, without a difference in the implant platform damage.
- Evaluation of desmoglein 1 and 3 autoantibodies in pemphigus vulgaris : correlation with disease severity(2020) Delavarian, Zarha; Layegh, Pouran; Pakfetrat, Atessa; Zarghi, Nazila; Khorashadizadeh, Mahboubeh; Ghazi, AlaPemphigus is an autoimmune blistering disease of the skin and mucous membranes caused by autoantibodies against desmoglein 1 (Dsg1) and desmoglein 3 (Dsg3). Pemphigus vulgaris (PV) is the most common form of pemphigus. The aim of this study was to assess the correlation between the levels of anti-desmoglein 1 and 3 autoantibodies and the severity of PV disease. Nineteen newly diagnosed patients with pemphigus vulgaris were enrolled in this study. The titers of Dsg in subjects by using enzyme-linked immunosorbent assay (ELISA) were done at diagnosis time-point, 4th and 8th weeks after the initiation of treatment, and the correlation of antibodies with the oral and skin disease severity was evaluated. The severity of cutaneous lesions was significantly correlated with anti-Dsg1 titer in all visits and the severity of mucosal lesions was correlated with the titer of Dsg3 in the third visit (<0.001, 0.001, 0.016 and 0.015 P value, respectively). Anti-Dsg-1 autoantibodies titers seem to be more useful in showing the extent of the disease and activity in pemphigus with mucocutaneous lesions.
- Extra short 4mm implants used to rehabilitation of atrophic posterior mandible. A serial case reports(2020) Ramos, Alysson-Henrique-Neves; Cornacchia, Gianfilippo-Machado; Nunes, Eduardo; Cosso, Mauricio-Greco; Souza, Leandro N.; Zenóbio, Elton GonçalvesRehabilitation of patients through implants in areas with severe bone resorption in the posterior mandible is a challenge in implant dentistry. In this context, extra short implants configure a treatment option for this type of patient, as they can avoid increased financial cost, treatment time and patient morbidity. The present study evaluated the marginal bone stability in individualized extra-short implants for masticatory function in the posterior mandible. Using digitized periapical radiographs of 13 extra-short implants performed on 7 patients. The mesial and distal regions of each implant were selected, from the bone crest to the region parallel to the apex, and the bone stability of this crest was measured using the Image J software immediate T1 and 1 year after rehabilitation (T2). The height of the bone crest remained stable, showing no statistically significant difference between T1 and T2 (p> 0.005) for both the mesial bone crest and the distal bone crest in individual or united crowns rehabilitation. Marginal bone stability was observed in extra short implants, corroborating the biological and biomechanical stability of these implants presented in the literature. Despite the limited sample size and proservation time, extra-short implants are predictive treatment options for patients with severe bone atrophy in the posterior mandible.
- The effect of osseodensification on implant stability and bone density : a prospective observational study(2020) Hindi, Aseel R.; Bede, Salwan Y.The aims of this study were to evaluate the effect of implant site preparation in low-density bone using osseodensification method in terms of implant stability changes during the osseous healing period and peri-implant bone density using CBCT. This prospective observational clinical study included 24 patients who received 46 dental implants that were installed in low-density bone using the osseodensification method. CBCT was used to measure the bone density pre- and postoperatively and implant stability was measured using Periotest® immediately after implant insertion and then after 6 weeks and 12 weeks postoperatively. The data were analyzed using paired t-test and the probability value <0.05 was considered statistically significant. Of the 46 implants, 43 were osseointegrated making the early survival of the implants 93.5%. There was a significant increase in bone density postoperatively; 337.6 ±182.9 compared to 265.3 ±173.9 Hounsfield units preoperatively. The primary implant stability was -2.7 ± 2.13 Periotest values (PTV), at the 6th week it decreased significantly (p<0.0001) to become 0.7 (± 4) PTV, and at the 12th week (secondary stability) it increased significantly (p<0.0001) to become -2.1 (± 2.8) PTV. The difference between primary and secondary stability was statistically non-significant (p=0.0814). Osseodensification resulted in high primary stability and increased peri-implant bone density but it did not prevent the implant stability drop during the first 6 weeks after insertion of implants.
- Angina bullosa hemorrhagica, an uncommon oral disorder. Report of 4 cases(2020) Alberdi Navarro, Javier; García García, Abel; Cardona Tortajada, Francisco; Gainza Cirauqui, María Luisa; Aguirre Urízar, José ManuelAngina bullosa hemorrhagica (ABH) is a rare oral disorder characterized by blood-filled bullous lesions in the oral cavity and the oropharynx in the absence of an underlying systemic, haematological or mucocutaneous condition. The presentation of the lesions is acute and located on the lining mucosa, mainly on the soft palate. Often, these lesions are single and rupture easily leaving an ulcerated area. In this study, we present 4 ABH cases in 3 women and 1 man and we discuss the main clinicopathological characteristics. The characteristics of this disorder are important to recognize in order to differentiate the lesions from other oral bullous conditions of the oral cavity such as mucocutaneous disorders or blood coagulation disorders.
- In vitro evaluation of composite resin fluorescence after natural aging(2020) Garrido, Talissa-Mayer; Hoshino, Lidiane-Vizioli-de Castro; Hirata, Ronaldo; Sato, Francielle; Neto, Antonio-Medina; Guidini, Victor-Hugo-Fazoli; Terada, Raquel-Sano-SugaSome composite resins contain luminophorous agents in order to reproduce tooth fluorescence. The objective of this study was to compare the fluorescence spectra emitted by composite resins with those of human enamel and dentin, and their emission behaviour after a 90-day natural aging period. Nine shades of the composite resins Z350XT/3M (XT), Opallis/FGM (OP) and Empress Direct/Ivoclar-Vivadent (ED) were analyzed. Five specimens (10.0 mm x 2.0mm) were fabricated for each shade. Enamel (5.0 mm x 0.30 mm) and dentin (5.0 mm x 1.0 mm) specimens were obtained from sound human third molars. Fluorescence spectra of human dentin and enamel as well as the composite specimens immediately after fabrication were measured at the excitation peaks of 375, 395 and 410 nm. To assess composite resin fluorescence intensity changes over time, measurements were conducted after 30, 60 and 90 days, at 395 nm. Differences in fluorescence intensity over time were analyzed with ANOVA and Tukey?s test (p<0.05). Fluorescence spectra baseline values of composites demonstrated no differences in intensity among the excitation peaks tested, with maximum emission found at the peak of 450 nm. Enamel and dentin spectra varied with different excitations, and the greater the excitation, the longer the wavelength in comparison to composite resins. After 90 days, XT presented an increase in fluorescence intensity, while OP and ED showed a reduction when compared with baseline values. Fluorescence intensity of composite resins changed during the period analyzed, with an emission behavior different from that of human enamel and dentin. The main changes occurred in the first 30 days.
- Analysis of salivary detection of P16INK4A and RASSF1A promoter gene methylation and its association with oral squamous cell carcinoma in a Colombian population(2020) González-Pérez, Leonor-Victoria; Isaza, Diana María; Arango-Pérez, Eduin-Alonso; Tobón-Arroyave, Sergio-IvánEpigenetic factors play a fundamental role in the etiopathogenesis of oral squamous cell carcinoma (OSCC). This study evaluated if salivary detection of P16INK4A/RASSF1A gene promoter methylation might be linked to the clinical/histological features of OSCC in a Colombian population. Methylation-specific polymerase chain reaction (MSP-PCR) was used to detect the methylation frequency of P16INK4A/RASSF1A genes in DNA obtained from whole saliva collected of 40 healthy controls (HC) and 43 OSCC patients. Determination of the clinical performance of MSP-PCR assay was based on standard algorithms derived from two-way contingency table analysis. The association of methylation status of targeted genes with OSCC was analyzed in a multivariate binary logistic regression model. There were significantly higher proportions of promoter methylation of these target genes in OSCC patients when compared with HC. The analysis of single methylated genes showed high specificity, good positive and negative predictive values, but was accompanied by a low sensitivity. OSCC cases with clinical stage III/IV, poorly differentiated, and severe cellular atypia showed a significantly greater proportion of methylated than that of unmethylated targeted genes in saliva samples. Logistic regression analysis indicated an independent association of P16INK4A and RASSF1A promoter methylation with OSCC diagnosis. A significant interaction effect between ageing and P16INK4A promoter methylation was also detected. Salivary detection of P16INK4A and RASSF1A promoter methylation appears to be independently associated with OSCC and may be linked to the tumor activity in the present population. Consequently, the targeting of these genes in saliva samples might constitute an important tool for diagnosis and prognosis purposes.
- The importance of bioceramics and computed tomography in the late clinical management of a horizontal root fracture : a case report(2020) Girelli, Caroline-Felipe-Magalhães; de Lima, Carolina-Oliveira; Lacerda, Mariane-Floriano-Lopes-Santos; Coellho, Renato-Girelli; Silveira, Frank-Ferreira; Nunes, EduardoRoot fractures resulting from dental trauma involve dentin, cementum and pulp. The present study aimed to demonstrate the importance of cone-beam computed tomography (CBTC) and bioceramics in the correct planning and intervention of a horizontal root fracture case in tooth 11 with late treatment in an 18-year-old patient. Clinical and radiographic examinations revealed tooth displacement, pain on vertical percussion and images suggestive of a horizontal root fracture. Pulp necrosis was diagnosed and CBTC was requested for treatment planning. Subsequently, endodontic treatment was performed using a bioceramic apical plug. A 2-year follow-up indicated the absence of root resorption and normal periodontal and periapical tissues. It was concluded that endodontic treatment associated with the use of bioceramics and the aid of CBTC is an effective therapeutic option in cases of permanent horizontal root fractures.
- Effect of silver diamine fluoride on the bond durability of normal and carious dentin(2020) Firouzmandi, Maryam; Mohaghegh, Mina; Jafarpisheh, MaedehSilver diamine fluoride (SDF) has attracted attention because of its clinical success in arresting dentin caries. It has been shown that it can inhibit matrix metalloproteinases and cysteine cathepsins. These two properties might be beneficial in bonding to caries-affected dentin (CAD). Therefore, the present study aimed to investigate the effect of SDF on the durability and bond strength to the CAD. Forty-eight third molars with occlusal caries were used. The roots were cut, and the occlusal enamel was removed. The CAD area surrounded by the normal dentin (ND) was exposed. All the specimens were bonded with an etch-and-rinse adhesive, but half of them were treated with SDF after acid etching (NT or SDF). Two cylinders of composite (0.9 mm in diameter and 0.7 mm in height) were built-up on each specimen, one on the CAD and the other on the ND area. The specimens in each group (NT-ND, NT, CAD, SDF-ND, SDF-CAD) (n=24) were equally divided into two subgroups. One subgroup was tested for microshear bond strength 24 hours after bonding (T0), and the other subgroup was tested after six months of water storage (T6). The bond strength data were analyzed using three-way ANOVA. Subgroup analysis was performed using independent samples t-test. Fracture patterns were also evaluated. At T0, the bond strength of NT-ND was higher than that of NT-CAD (p<0.00), but the bond strength of SDF-ND and SDF-CAD were not different (p=0.77). Comparison of the bond strength of the groups between the two time intervals yielded the following results: NT-ND-T0> NT-ND-T6 (p=0.04), SDF-ND-T0=SDF-ND-T6 (p=0.39), NT-CAD-T0=NT-CAD-T6 (p=0.51) and SDF-CAD-T0>SDF-CAD-T6 (p<0.00). The SDF treatment increased the bond strength to CAD but did not affect the bond strength to the ND. SDF hindered the decrease in the bond strength to the ND caused by aging. However, the effect of SDF on increasing the bond strength to CAD disappeared after aging.
- Morphological patterns of circumpulpal dentin affected by radiation-related caries(2020) Faustino, Isabel-Schausltz-Pereira; Palmier, Natalia; Fernandes, Patrícia-Maria; Ribeiro, Ana-Carolina-Prado; Brandao, Thais-Bianca; Santos Silva, Alan Roger; Vargas, Pablo Agustín; Lopes, Márcio AjudarteThe aim of this work was to evaluate the microscopic characteristics through polarized light microscopy, scanning electron microscopy and the mineral content of circumpulpal dentin of irradiated (IT) and non-irradiated teeth (NIT), with deep caries that reached the root canal. A total of 25 IT were analyzed macroscopically, and radiographed. 5 NIT were used as controls. Two 100-?m-thick sections, per specimen, were evaluated in a polarized light microscopy and a scanning electron microscope. Demographics and clinical data were collected. The results did not demonstrate distinct morphology of the IT compared with NIT. Mineral content values by weight percentage of Ca and P were also similar, corresponding to 66.65% and 33.21% in the IT and 66.60% and 33.29% in the NIT. The Ca/P ratio did not show statistical differences between groups being respectively 2.74 and 2.72, in the IT and NIT (p> 0.05). Radiotherapy does not change morphology and mineral content of circumpulpal dentin in IT.
- Influence of preheating on mechanical and surface properties of nanofilled resin composites(2020) Elkaffass, Ali-Atef; Eltoukhy, Radwa-Ibrahim; Elnegoly, Salwa-Abd-Elraof; Mahmoud, Salah-HassabResin composite preheating is an innovative method that could be clinically beneficial by improving the handling properties, marginal adaptation, and surface properties of uncured nanofilled resin composite materials. There is conflict and unclear information regarding the effect of preheating on the microhardness, fracture toughness and surface roughness of nanofilled resin composites. Thus, it is important to assess whether dental clinicians can adopt preheating procedures without compromising composite mechanical strength. Objective: The purpose of this study was to evaluate the effect of preheating on microhardness, fracture toughness and surface roughness of nanofilled resin composite. In this study, one commercial nanofilled resin composite Filtek Z350 XT was used. A total of 28 disc-shaped specimens were fabricated in a Teflon mold (10 mm diameter x 2 mm thick) for Vickers microhardness indentation test and surface roughness test. The samples were divided into two groups of 14 samples each, one group of samples was light-cured at room temperature (24ºC) without preheating (non-heated group), and the other group was light-cured after preheating (preheated group). Vickers hardness measurements of 14 specimens (n=7) either preheated or non-heated of the top and bottom surfaces was measured by means of microhardness tester by applying 100 g load for 10 s. Surface Roughness measurements (Ra) were obtained from 14 specimens (n=7) either preheated or non-heated with the atomic force microscope. Fourteen single-edge-notched-beam specimens were prepared for fracture toughness test (n=7) either preheated or non-heated with measurements (2.5 x 5 x 25 mm3) and a crack 2.12 mm in length. The specimens were tested via three-point bending mode, using a universal testing machine at crosshead speed of 1.0 mm/min until failure occurred. Independent sample t- tests revealed no significant difference between non-heated and preheated groups for all tests (p>0.05). However, for Vickers hardness test, there were significant differences between top and bottom surfaces for non-heated and preheated groups (p<0.05). Moreover, surface roughness average Ra (nm) mean values of preheated group was higher than non-heated group but no significant difference between them was found (p>0.05). Preheating procedure did not negatively affect microhardness, fracture toughness and surface roughness of nanofilled resin composites so preheating is recommended for the other potential clinical advantages.
- Optical magnification has no benefits on the detection of occlusal caries lesions in permanent molars using different visual scoring systems : an in vitro study(2020) Wilde, Sabrina; de Campos, Priscila-Hernandez; Marcondes, Ana-Paula-Marçal; Moura Netto, Cacio; Novaes, Tatiane-Fernandes; Lussi, Adrian; Diniz, Michele B.Some studies have addressed the influence of optical magnification on the detection of caries lesions using a visual scoring system. However, there is a lack of research related to the use of the CAST and ADA-CCS visual scoring systems. In addition, the reliability and accuracy of ADA-CCS index in permanent teeth were not studied yet. So, the aim of this study was to evaluate, in vitro, the influence of different levels of optical magnification on the detection of occlusal caries lesions in permanent molars using three visual scoring systems. One occlusal site per tooth was analyzed in 120 extracted permanent molars. Two trained examiners inspected the teeth using ICDAS (International Caries Detection and Assessment System), CAST (Caries Assessment Spectrum and Treatment), and ADA-CCS (American Dental Association-Caries Classification System) visual criteria, twice with each scoring system, with a one-week interval between examinations. The study was conducted in three phases: (A) without optical magnification, (B) using a binocular lens (3.5× magnification), and (C) using an operating microscope (16× magnification). Then, the teeth were sectioned longitudinally through the center of the selected site and the section with the more severe lesion was histological evaluated considering the D1 (lesions in enamel and dentin) and D3 (dentin lesions) thresholds. Kappa values for intra- and inter-examiner reproducibility were good to excellent for all systems. At the D1 threshold, sensitivity, accuracy, and area under the ROC curve were high for ICDAS and CAST in all phases. However, this was not the case for the ADA-CCS in phase C (<0.05). At the D3 diagnostic threshold, there was no significant difference between the visual scoring systems during the study phases (>0.05). The magnification does not improve the accuracy of the visual scoring systems in the detection of occlusal caries lesions in permanent molars.
- Comparison of salivary cytokines levels among individuals with Down syndrome, cerebral palsy and normoactive(2020) Habibe, Carolina-Hartung; Yoshida, Rosemeire-Arai; Gorjão, Renata; Gutierrez, Gabriela-Mancia; Heller, Debora; Birbrair, Alexander; Santos, Maria-Teresa-Botti-RodriguesIndividuals with Down syndrome (DS) present increased susceptibility to infections and high prevalence of periodontal disease. The objective of this study is to evaluate the salivary concentrations of IL-1?, IL-6, IL-8, IL-10, TNF? and IL-12p70 of DS individuals and compare to cerebral palsy (CP) and normoactive patients (all with gingivitis). Twenty-two individuals with DS, 24 with CP and 22 normoactive participated in this cross-sectional study. Salivary flow rate, osmolality rate, Oral Hygiene Index, Gingival Index (GI) and salivary inflammatory markers IL-1?, IL-6, IL-8, IL-10, TNF? and IL-12p70 were evaluated. Shapiro-Wilks, Chi-square, ANOVA One-Way and Kruskal Wallis tests were applied with significance level at 5%. The groups were homogenous for gender, age, and IL12p70 cytokine (p>0.05). GI was significantly higher in DS compared to CP and healthy (p<0.05). CP presented reduced salivary flow and increased osmolality rate. CP showed significantly higher values for TNF?, IL10, and IL6 compared to DS and normoactive (p<0.05). DS and CP presented significantly higher values of IL-1? and IL8 compared to normoactive (p<0.05). Individuals with CP have higher risk to develop periodontal disease due to reduced salivary flow rate, increased salivary osmolality rate and elevated TNF?, IL-10, IL-6 compared to DS.
- Impact of chronic allergic rhinitis on bite force and electromyographic activity of masseter and temporalis muscles of adult women(2020) Bordignon, Natalia-Augusta-Ferreira; Regalo, Simone; Vasconcelos, Paulo; Prandi, Marcos-Vinicios-Ribeiro; Hotta, Takami-Hirono; Gonçalves, Ligia; Regalo, Isabela; Siéssere, Selma; Palinkas, MarceloThe aim of this study was to analyse the stomatognathic system of adult women with chronic allergic rhinitis by means of molar bite force and electromyographic activity of the masseter and temporalis muscles. A total of 26 subjects were screened and divided into two distinct groups: chronic allergic rhinitis group (n = 13) and healthy control group (n = 13). Subjects were assessed by maximal molar bite force (right and left) and normalized electromyographic activity of mandibular tasks (rest, right and left laterality, protrusion and maximal voluntary contraction). Data were submitted to Student?s t test (p< .05). There was significant difference in right (p = .03) and left (p = .04) maximal molar bite force with force reduction in the chronic allergic rhinitis group. There was significant difference in normalized electromyographic activity in maximal voluntary contraction in the right (p =.01) and left (p = .01) temporalis muscles, with increased electromyographic activity in the masticatory muscles for the chronic allergic rhinitis group. The results suggest that chronic allergic rhinitis in adult women promoted negative changes in the electromyographic activity of temporalis muscles in maximal voluntary contraction and maximal molar bite force.
