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Language Journal of Clinical and Experimental Dentistry. 2017. Vol. 9, no. 1
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- A cephalometric comparison of twin block and bionator appliances in treatment of class II malocclusion(2017) Ahmadian Babaki, Fatemeh; Araghbidi Kashani, S. Mehdi; Mokhtari, SaeedehClass II malocclusion is one of the most common orthodontic problems. In cases of class II malocclusion with mandibular deficiency, functional appliances often are used with the intent of stimulating mandibular growth. Bionator and twin block are two of the more popular functional appliances. The aim of this study was to compare the treatment outcomes of these two appliances using cephalometric radiographs. Cephalometric radiographs of 33 patients who had class II division I malocclusion, before and after treatment were digitalized. The mean changes in twin block and bionator groups were compared using independent t test. Twin block and bionator showed no statistically significant differences in cephalometric parameters except for ANB, NA-Pog, Basal and Ar-Go-Me angles. There were no statistically significant differences in dentoalveolar and mandibular position between twin block and bionator (p>0.1). Twin block was more efficient in inhibition of forward movement of maxilla (p<0.1).
- The influence of length of implant on primary stability: an in vitro study using resonance frequency analysis(2017) Bataineh, Anwar B.; Al-dakes, Ala M.Primary stabilityis not sufficientin less contact area between the implant and bone, the healing process because will be disrupted due to micro-motions and fibrous tissue affects osseointegration. We implemented an in vitro experimental study of total 135 XiVE® implants were inserted in 22.5 bovine cow ribs with bone quality similar to a type IV human bone. Each rib end received a group of three different implant lengths, which were 8mm, 13mm and 15mm and had the same diameter 3.8mm. Immediately after the implant placement, its primary stability was measured using Osstell Mentor equipment. ANOVA Tukey’s honest to test the significant difference were performed for data analysis between the resonance measures of the different lengths of implants. Statistical significance was assessed at a level P< 0.05. A total of 45 implants were inserted for each length at cortical bone level. A significant difference between the three groups in favor of implant with 15mm length group (P = 0.000). Increasing dental implant length is considered to play a fundamental role in increasing dental implant primary stability, even in poor bone quality, through controlling the bone preparation process.
- Oral health literacy: how much Italian people know about the dental hygienist(2017) Pippi, Roberto; Bagnato, Flavia; Ottolenghi, LiviaPeople with poor OHL have the highest level of oral diseases and the worst oral treatment results. The main aim of the present study was to investigate the degree of knowledge of the role of the dental hygienist in patients who go to a public dental facility for the first time. A semi-structured questionnaire was administered to the patients with the “face-to-face” mode during a 12-month period. The principal component analysis, the general linear model and the chi-square test were used for the statistical analysis. A total number of 900 questionnaires were completed. Sixty-seven per cent of patients know that a specific degree is needed to practice dentistry and 93.1% of them know that a specific educational qualification is required to practice the dental hygienist profession. Sixty-three per cent of the subjects were aware of dental hygienist’s activities. There is no patient preference of gender as far as both dentist (84.11%) and dental hygienist (85.11%) are concerned. Seventy-five per cent of patients claimed to know what “dental hygiene” means and 65% of them believed that a good level of oral hygiene was important for oral disease prevention. Both qualification and marital status of patients are significantly associated with the patient’s level of knowledge of the dental hygienist profession. Patients with “High” scholastic qualifications showed significantly higher scores than those with “Low” qualifications. Married patients have less knowledge than widows/widowers, while divorced patients have greater knowledge than widows/widowers. Patients’ educational qualification itself only partially justifies the apparent high level of knowledge of patients about the dental hygienist’s role.
- Cervical necrotizing fasciitis and acute mediastinitis of odontogenic origin: a case series(2017) Cariati, Paolo; Monsalve Iglesias, Fernando; Cabello Serrano, Almudena; Valencia Laseca, Alfredo; García Medina, BlasNecrotising fasciitis (NF) is an uncommon infection. Early signs and symptoms include fever, severe pain and swelling, and redness at the wound site. Moreover, fulminant evolution and high mortality rate are typical of this pathology. In the present report we describes three cases of cervical necrotizing fasciitis complicated by acute mediastinitis. All patients were apparently immunocompetent adults. The main aim of the present report is to show the serious consequences that a dental infection might trigger. Furthermore, we highlight the importance of a multidisciplinary approach in these cases. The constant interaction between different medical specialties is essential for ensuring a proper management of each case.
- Effect of dental restorative materials on total antioxidant capacity and calcium concentration of unstimulated saliva(2017) Ramezani, Gholam H.; Moghadam, Mona Momeni; Saghiri, Mohammad-Ali; Garcia-Godoy, Franklin; Asatourian, Armen; Aminsobhani, Mohsen; Scarbecz, Mark; Sheibani, NaderTo evaluate the effect of dental amalgam and composite restorations on total antioxidant capacity (TAC) and calcium (Ca) ion concentration of unstimulated saliva. Forty-eight children aged 6-10 years selected and divided into three groups of sixteen (8 males, 8 females). In group A and B, samples consisted of two class II dental composite or amalgam restorations, while in group C samples were caries-free (control group). Unstimulated saliva from all samples was collected and TAC was measured by spectrophotometry using an adaptation of 2, 2’-azino-di-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) assay. The Ca ion level was estimated by an auto- analyzer. Data were analyzed with one- and two-way ANOVA test, at a p<.05 level of significance. Composite samples showed significantly higher TAC and lower Ca ion levels compared to amalgam and caries-free samples (p<.05). The TAC values showed only significant difference between groups (p<.05), while the Ca ion results showed significant differences within and between groups (p<.05). Dental composite restorations increased TAC and decreased Ca ion levels more than amalgam restorations in saliva. Gender is an effective factor in changes induced in oral cavity as females showed more emphatic reaction to dental filling materials than males. Patients who have dental restorations, especially dental composites, should pay more attention to their dental hygiene, because dental restorations can increase oxidative stress and decrease Ca ion level in saliva, which might jeopardize remineralization process of tooth structures after demineralization.
- Neurotoxicity evaluation of three root canal sealers on cultured rat trigeminal ganglion neurons(2017) Er, Kürşat; Ayar, Ahmet; Kalkan, Omer-Faruk; Canpolat, Sinan; Taçdemir, Tamer; Özan, ÜlküThe aim of this study was to investigate the possible neurotoxic effects of 3 root canal sealers (RCSs) (AH Plus, GuttaFlow, iRoot SP) on cultured rat trigeminal ganglion (TG) neurons. Primary cultures of TG neurons were obtained from 1 to 2-day old rats. Freshly mixed RCSs were incubated in sterile phosphate buffered saline and cells were incubated with supernatants of the RCSs for different time intervals (1-, 3-, 6- and 24-h; 1 or 1/10 diluted) and viability/cytotoxicity was tested by counting the number of live cells. Pair of dishes with cells from the same culture incubated with only culture medium was considered as negative controls. Cell images were captured and acquired at x200 magnification using a microscope equipped with a camera using special image program. The viable cells were manually counted assigned from the images for each dose and incubation duration. Data was analysed by using 1-way analysis of variance with Tukey post hoc tests. There was no significant change in cell viability after short duration of incubation (1- and 3-h) with the supernatant of any of RCSs, except for undiluted-AH Plus at 3-h. When AH Plus was compared with other RCSs, for diluted supernatants, there was only significant difference between iRoot SP and AH Plus at 24-h (P<0.05). Whereas undiluted-AH Plus was significantly more cytotoxic for 3-, 6- and 24-h periods as compared to respective incubation periods of undiluted other groups (P<0.05). GuttaFlow groups had similar neurotoxic effect on cells for all test periods. All tested RCSs exhibited a variable degree of neurotoxicity on these primary sensory neurons of orofacial tissues, depending on their chemical compositions. GuttaFlow and iRoot SP evoked a less toxic response to TG cells than AH Plus.
- Sealing efficacy of mineral trioxide aggregate with and without nanosilver for root end filling: an in vitro bacterial leakage study(2017) Eskandarinezhad, Mahsa; Shahvaghar-Asl, Naiemeh; Sharghi, Reza; Shirazi, Sajjad; Shakouie, Sahar; Salem Milani, Amin; Balaei, EsrafilVarious materials have been added to mineral trioxide aggregate to enhance its properties. This study was aimed to compare the sealing efficacy of MTA with and without nanosilver using bacterial leakage approach. Seventy canine teeth were prepared and obturated. Then, after apical resection, the root-end cavities were prepared by ultrasonic retrotips. Teeth were randomly divided into 4 groups containing two experimental groups (n=30) and two negative and positive controls (n=5). In group 1 and 2, root-end cavities were respectively filled with MTA and MTA with nanosilver (by 1% weight). Leakage assessment was carried out by bacterial leakage apparatus with Enterococcus faecalis species. Leakage comparison between experimental groups was done using Mann-Whitney test by Spss 16 software at significancy level of 0.05. The median bacterial leakages for MTA and MTA with nanosilver were 19 and 2, respectively. The mean bacterial leakages for MTA and MTA with nanosilver were 30.06±28.67 and 9.66±14.25, respectively. Mann-Whitney test indicated that there was a significant difference in bacterial leakage day between two experimental groups (P=0.002). Based on the findings of this in-vitro bacterial leakage study, adding nanosilver to MTA decreased its sealing ability.
- Effect of bleaching agents on enamel surface of bovine teeth: a SEM study(2017) Pimenta Dutra, Ana Cristina; Albuquerque, Rodrigo de Castro; Morgan, Luís Fernando dos Santos Alves; Pereira, Geraldo Magela; Nunes, Eduardo; Horta, Martinho Campolina Rebello; Silveira, Frank-FerreiraThis study aimed to evaluate changes in the enamel surface of bovine teeth after whitening with exogenous bleaching agents: 10% carbamide peroxide (group 1), 16% carbamide peroxide (group 2) and 35% hydrogen peroxide activated by a light-emitting diode (LED) (group 3). The evaluations were performed by scanning electron microscopy (SEM). Ninety bovine teeth were divided into five groups (n = 18). The bleaching agents 10% and 16% carbamide peroxide were applied for eight hours a day for 14 consecutive days. The third agent, LED-activated 35% hydrogen peroxide, was used four times at seven-day intervals. Each of the four time points consisted of three applications of 10 minutes each. A 37% phosphoric acid solution and artificial saliva were used as positive and negative controls, respectively. The evaluations by SEM showed changes in the enamel surfaces of the specimens. Based on the Mann-Whitney statistical test, the data showed significant differences (p<0.05) between groups 1 and 2 and between groups 2 and 3. However, no significant difference (p>0.05) was observed between groups 1 and 3. Based on these results, it can be concluded that bleaching agents can cause changes in the structure of tooth enamel and that these changes are related to the concentration and the duration of contact with the tooth surface.
- Frequency of craniofacial pain in patients with ischemic heart disease(2017) Bakhshi, Mahin; Rezaei, Rezvan; Baharvand, Maryam; Bakhtiari, SedighehReferred craniofacial pain of cardiac origin might be the only symptom of ischemic heart accidents. This study aimed to determine the frequency of craniofacial pain in patients with ischemic heart disease. This cross-sectional study was accomplished on 296 patients who met the criteria of having ischemic heart disease. Data regarding demographics, medical history and referred craniofacial pain were recorded in data forms. In addition, patients underwent oral examination to preclude any source of dental origin. Chi-square test, Student’s t-test and backward regression model were used to analyze the data by means of SPSS software version 21. P<0.05 was considered significant. A total of 296 patients were studied comprising of 211 men (71%) and 85 women (29%) with the mean age of 55.8. Craniofacial pain was experienced by 53 patients out of 296, 35 (66%) of whom were male and 18 (34%) were female. None of the patients experienced craniofacial pain solely. The most common sites of craniofacial pain were occipital and posterior neck (52.8%), head (43.3%), throat and anterior neck (41.5%) respectively. We found no relationship between craniofacial pain of cardiac origin with age, diabetes, hypertension, and family history. On the other hand, there was a significant relationship between hyperlipidemia and smoking with craniofacial pain of cardiac origin. Radiating pain to face and head can be expected quite commonly during a cardiac ischemic event. Dental practitioners should be thoroughly aware of this symptomatology to prevent misdirected dental treatment and delay of medical care.
- Preventive effects of different protective agents on dentin erosion: an in vitro investigation(2017) Poggio, Claudio; Gulino, Chiara; Mirando, Maria; Colombo, Marco; Pietrocola, GiampieroThe purpose of this in vitro study was to evaluate the preventive effects of different protective agents on dentine erosion, measuring mean percentage weight loss. Dissolution of dentine under erosive challenges caused by soft drinks was analyzed: specimens were weighed following each immersion period, with mean percent weight losses calculated. Extracted teeth were sectioned into uniform slabs. Seventy permanent enamel specimens were randomly distributed to seven groups. Initial weights of all dentin specimens were performed. The fluoride pastes Remin Pro, MI Paste Plus, Tooth Mousse, Biorepair, Biorepair Plus and Regenerate were used in this study. A control group was treated just with tap water. The specimens then were immersed in Coca-Cola for a total of 32 min at room temperature. Finally each specimen was dry and weighed. The mass loss was calculated as a percentage of that observed prior the fluoride pastes application. Weight loss data were subjected to Analysis of Variance (One-way ANOVA) followed by Bonferroni’s post hoc tests. Percent weight loss of specimens exposed to early stages in Coca-Cola showed linear progression with time. Specimen’s application of fluoridated varnishes such as Biorepair or Regenerate, prior immersion in Coca-Cola, significantly protect dentin from demineralization. Otherwise, application of Tooth Mousse or Biorepair Plus increased dentin demineralization starting from 24 min of immersion in Coca-Cola. Despite the limitations of this study, the protective pastes that showed the less weight loss due to the acidic challenge are Biorepair and Regenerate.
- Age-related EBV positive clonal B-cell Lymphoid proliferation (EBV+-DLBCL)(2017) Georgakopoulou, Eleni A.; Doukas-Alexiou, Marina; Stoufi, Eleana; Kittas, Christos; Pangalis, Gerasimos; Laskaris, GeorgeThe Ebstein Barr virus(EBV), herpes virus 5 has been associated with lymphoproliferative disordrers. Age-related EBV+ B-LPD is defined as an EBV+ clonal B-cell lymphoid proliferation or EBV+-DLBCL developing in patients over the age of 40 years in the absence of any known immunodeficiency and without an underlying T-cell lymphoma1. We present a case of EBV+ clonal B-cell lymphoid proliferation.
- Intraosseous cavernous hemangioma in the mandible: a case report(2017) Elif, Bilgir; Derya, Yildirim; Gulperi, Kocer; Sevgi, BozovaIntraosseous vascular lesions are rare conditions. They are most commonly seen in the vertebral column and skull; nevertheless, the mandible is a quite rare location. In this report, we present a case of intraosseous cavernous hemangioma in the mandible and discuss the clinical and radiological features. A 28-year-old male patient attended to our clinic with a complaint of painless swelling of mandible. Clinical evaluation revealed a bone-hard, smooth-surfaced, immobile mass in the left mandibular lingual area. The patient was evaluated with panoramic and occlusal radiography and computed tomography. The lesion surgically excised and pathological examination revealed an intraosseous cavernous hemangioma. Follow-up imaging 1 year later with cone beam computed tomography revealed recurrence of the lesion. The conclusion of this paper; when a bone hard, well-shaped mass was seen in the mandible, the possibility of intraosseous hemangioma must be remembered and before surgical procedure detailed radiographic evaluation should be performed.
- A Cone Beam Computed Tomography (CBCT) evaluation of MB2 canals in endodontically treated permanent maxillary molars. A retrospective study in Indian population(2017) Shetty, Heeresh; Sontakke, Subodh; Karjodkar, Freny; Gupta, Pankaj; Mandwe, Ashish; Banga, K.SCurrent technological advances have allowed application of different study designs and techniques for investigation of dental anatomy. Some clinical studies have provided evidence that Cone Beam computed tomography (CBCT) scanning is an important resource in assessment of root canal systems notably to identify MB2 canals in maxillary molars as CBCT scans allow in vivo dental investigation in axial, sagittal and coronal planes simultaneously. The current study was undertaken to detect and evaluate filled/unfilled MB2 canals in endodontically treated, asymptomatic maxillary molars utilizing cone beam computed tomography (CBCT). A retrospective study of 100 CBCTs of patients were underwent scanning for various treatment modalities, with asymptomatic endodontically treated permanent first and second maxillary molars were selected. History of root canal treatment varied from minimum of 1 year to a maximum of 10 years. Axial and paraxial images obtained were used to assess the presence of MB2 canal. Paraxial images were used to assess the periapical status. Of the 100 scans, 66 were of permanent maxillary first molar and 34 were of permanent maxillary second molar. The incidence of MB2 canal was 86.36% in maxillary first molars and 29.4% in maxillary second molars. 77.19 % of maxillary first molars and 90% of maxillary second molars had an unfilled MB2 canal. 72.7% of maxillary first molars and 88.8% of maxillary second molars showed significant periapical radiolucencies in unfilled MB2 canals. MB2 canals were present in majority of cases and most of the unfilled MB2 canals showed evidence of periapical radiolucencies.
- Effect of tooth-bleaching on the carbonate concentration in dental enamel by Raman spectroscopy(2017) Vargas-Koudriavtsev, Tatiana; Herrera-Sancho, O.A.There are not many studies evaluating the effects of surface treatments at the molecular level. The aim of this in vitro study was to analyze the concentration of carbonate molecules in dental enamel by Raman spectroscopy after the application of in-office and home whitening agents. Sixty human teeth were randomly divided into six groups and exposed to three different home bleaching gels (Day White) and three in-office whitening agents (Zoom! Whitespeed and PolaOffice) according to the manufacturer´s instructions. The concentration of carbonate molecules in enamel was measured prior to and during the treatment by means of Raman spectroscopy. Statistical analysis included repeated measures analysis of variance (p≤0.05) and Bonferroni pairwise comparisons. At home bleaching agents depicted a decrease in the carbonate molecule. This decrease was statistically significant for the bleaching gel with the highest hydrogen peroxide concentration (p≤0,05). In-office whitening agents caused an increase in carbonate, which was significant for all three groups (p≤0,05). In-office bleaching gels seem to cause a gain in carbonate of the enamel structure, whilst at-home whitening gels caused a loss in carbonate.
- Effect of two contemporary root canal sealers on root canal dentin microhardness(2017) Khallaf, Maram E.Successful root canal treatment depends on proper cleaning, disinfecting and shaping of the root canal space. Pulpless teeth have lower dentin microhardness value compared to that of vital teeth. A material which can cause change in dentin composition may affect the microhardness. Thus the aim of this study was to evaluate and compare the effect of two root canal sealers on dentin microhardness. Forty two single rooted teeth were selected and divided into 3 equal groups; Apexit, iRootSP and control groups (n=14) Each group was then divided into 2 subgroups according to the post evaluation period; 1 week and 2 months (n=7). Root canal procedure was done in the experimental groups and obturation was made using either; Apexit, iRootSP or left unprepared and unobturated in the control group. Roots were sectioned transversely into cervical, middle and apical segments. The three sections of each root were mounted in a plastic chuck with acrylic resin. The coronal dentin surfaces of the root segments werepolished. Microhardness of each section was measured at 500 µm and 1000 µm from the canal lumen. Four way-ANOVA revealed that different tested sealer materials, canal third, measuring distance from the pulp and time as independent variables had statistically non significant effect on mean microhardness values (VHN) at p≤0.001. Among iRootSP groups there was a statistically significant difference between iRoot SP at coronal root portion (87.79±17.83) and iRoot SP at apical root portion (76.26±9.33) groups where (p=0.01). IRoot SP at coronal canal third had higher statistically significant mean microhardness value (87.79±17.83) compared to Apexit at coronal third (73.61±13.47) where (p=0.01). Root canal sealers do not affect dentin microhardness.
- Sealing efficacy of system B versus Thermafil and Guttacore obturation techniques evidenced by scintigraphic analysis(2017) Marques-Ferreira, Manuel; Abrantes, Margarida; Ferreira, Hugo Diogo; Caramelo, Francisco; Rabaça-Botelho, Maria-Filomena; Palmeirão-Carrilho, Eunice VirgíniaThis study compared root canal sealing ability, filled by Continuous Wave compaction and two carrier-based obturation systems, using the nuclear medicine approach. Fifty-five single-rooted extracted teeth were selected. The crowns were sectioned and each tooth was instrumented using rotary Protaper® Universal system. The roots were divided into 3 experimental groups and two control groups. Forty-five root canals were filled, using Continuous Wave, GuttaCore or Thermafil system and TopSeal sealer. Ten teeth were used as control. On the 7th days the apices were submersed in a solution of sodium pertechnetate 99mTc for 3 hours and the radioactivity was counted. Although apical leakage in the Continuous Wave group was lower compared with GuttaCore and Thermafil groups, there was no statistical difference (p>0.05). System B, GuttaCore and Thermafil techniques showed a similar sealing effect.
- Prophylaxis and antibiotic therapy in management protocols of patients treated with oral and intravenous bisphosphonates(2017) Bermúdez Bejarano, Elena Beatriz; Serrera Figallo, María Angeles; Gutiérrez Corrales, Aida; Romero Ruiz, Manuel María; Castillo de Oyagüe, Raquel; Gutiérrez Pérez, José Luis; Torres Lagares, DanielOsteonecrosis of the jaw (MRONJ) linked to bisphosphonate treatment has specific characteristics that render its therapeutic management challenging for clinicians. Poor response to standard treatment makes it essential to take special precautions when treating this type of disease; therefore, antibiotic prophylaxis and/or antibiotic therapy have been proposed as effective and helpful tools in these situations. This article seeks to assess published evidence in order to evaluate the different protocols used for antibiotic prophylaxis and/or antibiotic therapy in the general context of patients treated with bisphosphonates. A literature review of the last 10 years was carried out in PubMed using the following keywords: “antibiotic prophylaxis and osteonecrosis,” “bisphosphonates AND osteonecrosis AND dental management,” “bisphosphonate AND osteonecrosis AND antibiotic prophylaxis AND oral surgery.” A total of 188 articles were obtained, of which 18 were ultimately selected. In patients treated with oral and intravenous bisphosphonates without chemotherapy-associated osteonecrosis of the jaw, antibiotic prophylaxis prior to oral surgery is an important tool to avoid osteonecrosis and promote healing of the affected area. If the patient previously exhibited chemotherapy-associated osteonecrosis after tooth extraction, antibiotic prophylaxis is indicated to prevent recurrent osteonecrosis and promote healing of the extraction site. If chemotherapy-associated osteonecrosis is already present, antibiotic therapy is a vital part of conservative management to reduce the symptomatology of MRONJ and keep it from worsening. Finally, a lack of clinical data and randomized controlled trials makes it difficult to choose the most appropriate protocol for the various clinical situations studied.
- Torque expression in self-ligating orthodontic brackets and conventionally ligated brackets: a systematic review(2017) Al Thomali, Yousef; Noor-Mohammed, Roshan; Basha, SakeenabiTo evaluate the torque expression of self ligating (SL) orthodontic brackets and conventionally ligated brackets and the torque expression in active and passive SL brackets. Our systematic search included MEDLINE, EMBASE, CINAHL, PsychINFO, Scopus, and key journals and review articles; the date of the last search was April 4th 2016. We graded the methodological quality of the studies by means of the Quality Assessment Tool for Quantitative Studies, developed for the Effective Public Health Practice Project (EPHPP). In total, 87 studies were identified for screening, and 9 studies were eligible. The quality assessment rated one of the study as being of strong quality, 7 (77.78%) of these studies as being of moderate quality. Three out of 7 studies which compared SL and conventionally ligated brackets showed, conventionally ligated brackets with highest torque expression compared to SL brackets. Badawi showed active SL brackets with highest torque expression compared to passive SL brackets. Major and Brauchli showed no significant differences in torque expression of active and passive SL brackets. Conventionally ligated brackets presented with highest torque expression compared to SL brackets. Minor difference was recorded in a torque expression of active and passive SL brackets.
- Treatment of chronic pain associated with nocturnal bruxism with botulinum toxin. A prospective and randomized clinical study(2017) Al Wayli, HessaTo evaluate the role of botulinum toxin type A (BTX-A) in the treatment of pain associated with nocturnal bruxism. Fifty subjects reporting nocturnal bruxism were recruited for a randomized clinical trial. Twenty five bruxers were injected with botulinum toxin in both masseters, and twenty five were treated with traditional methods of treating bruxism. Patients were evaluated at 3rd week, 2nd and 6th month and one year after injection and then used to calculate bruxism events. Bruxism symptoms were investigated using questionnaires. Mean pain score due to Bruxism events in the masseter muscle decreased significantly in the botulinum toxin injection group A (P =0.000, highly significant). However, in the conventional treatment group, mean pain score does not show improvement with time (p>0.05). Our results suggest that botulinum toxin injection reduced the mean pain score and number of bruxism events, most likely by decreasing the muscle activity of masseter rather than affecting the central nervous system.
- Immunoexpression of Wnt/β-catenin signaling pathway proteins in ameloblastoma and calcifying cystic odontogenic tumor(2017) Dutra, Sabrina Nogueira; Pires, Fábio-Ramôa; Armada, Luciana; Azevedo, Rebeca SouzaWnt/β-catenin signaling pathway is essential for the beginning of odontogenesis and may be involved in the development and progression of some odontogenic tumors. Thus, the aim of this study was to comparatively evaluate the immunohistochemical expression of Wnt/β-catenin signaling pathway proteins in a series of AME and CCOT. Immunohistochemical reactions were performed using antibodies against Wnt1, Wnt5a and β-catenin in 17 cases of solid AME and 6 cases of CCOT. In the AME group, Wnt1 and Wnt5a were identified in the epithelium in most of the cases, and β-catenin was mainly identified in the cytoplasm of the tumoral cells. In the CCOT group, Wnt1 and Wnt5a were identified in the epithelium and in the ghost cells in almost all the cases, and β-catenin was mainly identified in the cytoplasm and in the nuclei of the tumoral cells. These results contribute to support the importance of Wnt/β-catenin signaling pathway proteins in AME and CCOT tumorigenesis. The abnormal expression of cytoplasmic and/or nuclear β-catenin appears to contribute to the development of both AME and CCOT. In addition, it is possible that Wnt1 and Wnt5a expression in ghost cells can contribute to its histogenesis in CCOT.
