Gaceta internacional de ciencias forenses. 2014. No. 13

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    A genetic method for diagnosing lantana poisoning using a barcoding-based, genusspecific, polymerase chain reaction
    (2014) Francés Bozal, Francesc
    INTRODUCTION: Lantana species are highly toxic as they contain lantadenes and other chemical compounds. While human poisonings have occasionally been reported, cattle throughout the world are often poisoned, which represents a serious loss of income in many countries. In order to reduce the mortality rate among lantana-poisoned animals, an early and reliable diagnosis is required. Our aim, therefore, is to develop a genetic test to determine the presence of Lantana Camara and other common lantana species in the content of the digestive tube and faecal products of cattle. MATERIAL AND METHODS: A comparison between Internal Transcribed Spacer 1, 2 and 5.8 subunit DNA sequences of Lantana Camara and all other species available in NCBI Nucleotide database has been undertaken using the BLAST tool. As a result, several DNA fragments, highly specific for Lantana were identified. PCR primers were designed to hybridize on these sequences. Finally, a pair of universal primers was used in order to achieve a constant band for all angiosperms. RESULTS: Our multiplex PCR design generated a constant band that certified the correct development of the PCR in all angiosperms tested. However, in the case of Lantana Camara DNA, a specific and lighter band was generated, allowing the positive identification of this species. DISCUSSION: Ours is the first specific test for confirming Lantana Camara poisoning reported to date, and may provide a useful tool to veterinary services in countries where lantana poisoning in cattle is frequent.
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    Valoración médico forense de muerte en custodia por delirium agitado: a propósito de un caso
    (2014) Navarro Escayola, Esperanza; Muñoz-Quirós Caballero, J.M.; Cardona Valencia, Begoña
    En este artículo exponemos los resultados de la autopsia de un varón joven que falleció en el hospital por un cuadro de delirium agitado. Es un síndrome caracterizado generalmente por agitación psicomotriz, agresividad y puede producir muerte súbita, en ocasiones durante la aplicación de métodos de contención mecánica por los Cuerpos de Seguridad del Estado o personal sanitario. Está típicamente asociado al consumo de drogas de abuso, como la cocaína. En estos casos, estará incluida en el grupo de muertes en custodia, que es aquella que se produce cuando la víctima se encuentra privada de libertad. Tiene una indudable repercusión médico legal, puesto que se considera prototipo de muerte sospechosa de criminalidad.
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    Desarrollo de un métode para identificación de guantes con policloruro de vinilo (pvc)
    (2014) Ascolani Yael, Julián E.; Pregliasco, Rodolfo G.
    En un cuerpo hallado en una casa, en las cavidades de la oreja se encontraron ``motas'' plásticas provenientes de guantes de algodón moteados. Asimismo, se encontraron motas del mismo tipo en los alrededores del cuerpo. En la casa de un sospechoso, se encontraron guantes a los cuales les faltaban algunas motas. La pregunta es si podemos evaluar si los guantes hallados en la casa del sospechoso tienen un origen común con las motas encontradas en la escena del crimen. Se hizo un estudio comparativo de los diferentes tipos de PVC utilizados para hacer guantes moteados, utilizando diferentes guantes habituales en el mercado. Se evalúa la capacidad discriminatoria del análisis químico por la técnica de EDS (utilizando un SEM) y térmico con técnicas calorimétricas (DSC). Se analizan además las características morfológicas y cómo varían las texturas dependiendo del proceso de fabricación. Finalmente, se sugiere una secuencia de análisis para hacer la identificación del plástico, de manera de ir confirmando progresivamente la posibilidad de un origen común. La secuencia implica realizar estudios progresivamente más complejos. La utilidad de este esquema es poder detenerse a tiempo en el caso de que la incompatibilidad de las fuentes esté demostrada. Esto permite reducir costos y evitar la redundancia.
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    Evolución del número de suicidios en España respecto a variables macroeconómicas
    (2014) Ramos Martín, Emma
    In certain countries, the suicide mortality supposes a problem of public health. In Spain, serveral studies have analyzed if the number of suicides has been modified due to the economic crisis, as a consequence of unemployment, low incomes, poverty, social inequality, cuts in social services, health or education, concluding that in Spain the suicide attemps have not increased since the years before the economic crisis, not being able to appreciate inmediate effects for the health and not being able to prove a causal connection between financial crisis and suicide. Nevertheless, other authors point out that there is an important relation between suicides and young age aswell as elders, a poor health and depression, that can be increased due to the economic crisis. In opposite, out of Spain, a study concludes that there exists a relation beween the recent suicide cases in the English regions with higher unemployment rates as a consequence of the economic crisis. Relating the british study and due to the fact that the spanish authors has mostly concentrate their efforts on determining the economic crisis influence on suicides, we try to analize, from a broader point of view, the possible existence of a casual relation between the main macroeconomic indicators and the suicide rates in Spain. For this purpose, the methodology used has been to extract data relating demography, causes of death (suicide and self-inflicted injury), GDP, CPI and unemployment in complete historical series sufficiently representative so as to be considered as sampling, between 1980 and 2012, analyzing the evolution of the mentioned variables as well as the statistical distribution and their possible correlation. Furthermore, we develope a gender distribution for the purpose of the determination whether differentiated behaviours exists. Following the analysis, in conformity with the consulted authors, the number of suicides seems to be independent of unemployment rate increase or, in any case, seems that this increase is inversely related to the number of suicides. Nevertheless, the studied macroeconomic variable that shows a higher correlation coefficient with regard to the suicide rate is GDP, with indeces that show that the evolution of the male suicide rate is highly related with GDP evolution and, on the contrary, the number of women's suicides represents a continued evolution and even slightly linear independently of the macroeconomic variables consulted. In terms of carrying out a prognosis about the total number of suicides, it is proposed to establish on the one hand, the male number of suicides according to the forecast for the development of the GDP and, on the other, the women number of suicides in linear geometric progression with the coefficients obtained.
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    Cursos de formación de bajo coste
    (2014) Castelló Ponce, Ana
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    Por qué demandan a los médicos en méxico y por qué nace el movimiento médico nacional #yosoy17
    (2014) Peña, José Antonio
    This article attempts to show why lawsuits against doctors are mostly due to the low investment by the Government of Mexico in terms of health. Furthermore, the influencing factors and the peaceful mobilization organisms, created in order to inform and extend the dissatisfaction of the people to the government authorities.
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    Si lo sé... ¿lo digo?
    (2014) Verdú Pascual, Fernando Alejo