Journal of Clinical and Experimental Dentistry. 2020. Vol. 12, no. 4

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    Plasmablastic lymphoma as a manifestation of the human immunodeficiency virus : case report
    (2020) Vera, Gonzalo; Córdova, Pablo; Sepulveda, Gerson; Benavides, Tatiana; Astorga, Felipe; González Arriaga, Wilfredo Alejandro
    Plasmablastic lymphoma is a rare subtype of non-Hodgkin?s lymphoma, which generally presents an aggressive clinical course and low survival rates. It is strongly associated with HIV infection and the most common site of involvement of the territory of the head and neck is Waldeyer?s lymphatic ring, followed by the gastrointestinal tract, lymph nodes and skin. The morphological characteristics of PBL in the oral cavity / jaw in the context of HIV infection show diffuse sheets of large immunoblastic cells with abundant cytoplasm, vesicular chromatin and prominent nucleus, a small located in the center with plasma cells differentiation. The main goal of this article is to review the literature of the plasmablastic lymphoma and report a case.
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    Advantages and limitations of implant surgery with CAD/CAM surgical guides : a literature review
    (2020) Unsal, Gokce Soganci; Turkylmaz, Ilser; Lakhia, Samantha
    Background: The purpose of this study is to review the available literature associated with implant surgery using computer-aided design/computer-aided manufacturing (CAD/CAM) surgical guides and discuss the advantages and disadvantages of this advanced technique. Material and Methods: An electronic literature search was conducted in the PubMed database for the relevant in-formation on implant placement with CAD/CAM surgical guides. This review was constructed following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. Articles were limited to those published within the past 10 years and in the English language. Only clinical studies were included. Inclusion cri-teria were: studies including 10 implants or more and studies presenting angular deviations in degrees and linear deviations in millimeter. Observational studies, reviews, animal studies, in vitro studies, case reports, simulation studies were excluded. Nine articles were included for qualitative synthesis. Results: The initial search detected 61 articles, and after screening abstracts, a total of 15 articles were selected for full-text review. After the full-text analysis of the 15 articles, six articles were excluded as they did not meet inclusion criteria for study design, study population, and implant placement with data presentation for angular and linear deviations. Ultimately, nine articles providing angular and linear deviations between planned and actual pla-ced implants were used in this review. Common problems that may be encountered by clinicians were listed, and recommendations were made on how to avoid those problems.Conclusions: It has been suggested that although unrealistic expectations are often associated with implant place-ment with CAD/CAM surgical guides, there is no impeccable accuracy in the clinic. This review demonstrated that the practitioners should be aware of the angular and linear deviations up to 5 ° and 2.3 mm. Therefore, inexperien-ced dentists should obtain adequate training and be familiar with the basic steps with CAD/CAM surgical guides to avoid complications.
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    Particulated wisdom teeth as an autologous bone substitute for grafting/filling material in bone defects : case Report
    (2020) Arabadzhiev, Ivan; Maurer, Peter; Stevao, Eber
    Introduction: Bone augmentation material using permanent teeth are confirmed in many articles to provide good histological and clinical results. Advances in osteoconduction and osteoinduction, low cost of material, no risk of disease transmission and elimination of foreign body reaction are important aspects not only to the clinician but to patient as well. Many techniques and devices to obtain graft material for bone augmentation out of teeth are found in the literature. Material and Methods: The wisdom teeth crush technique developed in our office and described in this case report is simple and no specific devices for teeth grinding were used. The graft material was obtained from impacted in-tact wisdom teeth without chemical conditioning of the particles with preservation of the dental pulp and the cells found in it. This study presents crushed autologous maxillary wisdom tooth as filling material in two bone defects in premaxilla caused by cysts removal. Results: The clinical results and Panoramic X-ray evaluation at three months postoperatively were very promising. Conclusions: Although further clinical researches are necessary to evaluate this substitutive for bone augmentation technique, the authors believe it can be safely used by oral/maxillofacial surgeons.
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    Prevalence of dental caries among 6 ? 12 year old schoolchildren in social marginated zones of Valencia, Spain
    (2020) Prada, Ilaria
    Background: To estimate the prevalence of caries and to study oral health habits (oral hygiene, toothbrushing fre-quency, cariogenic or no cariogenic diet, visits to dentist) in children aged 6 to 12 with social exclusion risk.Material and Methods: A cross sectional study was conducted in a sample of 160 children aged form 6 to 12 years belonging to Colegio Madre Petra in Torrent and Colegio Nuestra Señora de los Desamparados in Nazaret (Valen-cia). Among other variables DMFT and Greene and Vermilion simplified plaque index were analyzed. Results: The prevalence of caries observed was 81.87% and the global DMFT was 4.481. The mean plaque in-dex observed was 1.12. No statistically significant differences were found between global DMFT and sex, global DMFT and age, global DMFT and diet, global DMFT and visits to the dentist and global DMFT and plaque index. A significant association was seen between global DMFT and ethnicity and global DMFT and brushing frequency. Statistically significant association was found also between plaque index and visits to the dentist and plaque index and diet. Conclusions: It was observed that children at risk of social exclusion had a very high global DMFT and a regular plaque index. So, it can be concluded that social exclusion constitute an underlying factor that increase caries pre-valence and global DMFT and a marker of poor oral hygiene habits.
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    Synchronous occurrence of two lateral periodontal cysts in the same patient. Report of a rare case and review of the literature
    (2020) Karveleas, Ilias; Kalogirou, Eleni Marina; Tosios, Konstantinos I.; Nikitakis, Nikolaos G.
    We present a case of a patient with two lateral periodontal cysts in the maxilla and the mandible, respectively, and review the English literature on multiple lateral periodontal (LPCs) cysts and/or gingival cysts (GCs) and botryoid odontogenic cysts (BOCs). The patient was a 59 year-old female with two fluctuant swellings covered by semi-lucent mucosa on the attached gingiva between the maxillary and mandibular right canine and first premolar teeth, respectively. Periapical radiographs revealed at the respective sites between the roots of the canine and first premolar teeth areas unilocular radiolucencies. Intra-operatively, the presence of bone cavities was confirmed at both sites. The microscopic features were consistent with LPC. The review of the English literature on multiple LPCs and/or GCs and BOCs found seven reports of multiple LPCs, four of multiple GCs, and two with an LPCs and a GC. It is concluded that multiple LPCs have been rarely reported in the literature, but should be included in the differential diagnosis of multifocal radiolucencies lateral to vital teeth. The possibility of multiple lesions in different locations should direct to a thorough clinical and radiographic examination in a patient diagnosed with an LPC or GC.
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    Resistance to demineralisation of adjacent enamel and dentine, fluoride release and dentine bond strength of fluoride-containing self-etch adhesive systems
    (2020) Pirmoradian, Maryam; Esmailzadeh, Shima; Davaie, Sotoudeh; Albakhakh, Bahaa Abdulrazzaq Jerri; Sanaee, Bahareh; Asgari, Elnaz; Shekofteh, Kiana; Habibzadeh, Sareh; Behroozibakhsh, Marjan
    Background: The current study aimed to assess the amount of fluoride released from fluoride-containing dental adhesives and its effect on micro-tensile bond strength (?TBS) and on resistance to demineralisation of dentine and enamel.Material and Methods: Two fluoride-containing dental adhesives, and a fluoride-free adhesive were used as expe-rimental adhesives. After thermal cycling the ?-TBS of adhesives to dentine and the failure mode were assessed. The fluoride release and cross-sectional microhardness (CSMH) of specimens were measured before and after one day, 7 and 28 days of pH-cycling. The data were analysed using one-way ANOVA, Weibull statistics and repeated measures ANOVA. Results: The results indicated a significant difference between the group of FL and both the SE and LBF groups (p?0.001). The CSMH values of both the dentine and enamel underneath the adhesives was reduced at 28 th day of the pH-cycling compared to the baseline (p?0.001). From day 1 to day 28, the released fluoride declined in both the fluoride containing dental adhesives (p?0.001).Conclusions: Based on the results, the released fluoride from dental adhesives may adversely influence the bond strength and durability of the resin/dentine interface. Moreover, the released fluoride didn?t improve the resistance to demineralisation of adjacent enamel and dentine to bond interface.
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    Rates of tooth movement and bone remodeling activity : self-ligating versus conventional brackets
    (2020) Nascimento, Leonard Euler Andrade Gomes do; Pithon, Matheus-Melo; Ruellas, Antônio Carlos de O.; Franzotti, Eduar do Sant`Anna; Filho, Antônio Cruz Gonçalves; de Souza, Margareth Maria Gomes; Bolognese, Ana Maria
    Background: Bracket systems have been developed with the purpose of reducing frictional resistance between the archwire and accessories. The aim of this research was to compare rates of tooth movement and regions of cellular bone modeling activity along tooth root surfaces of teeth moved with conventional vs. self-ligating brackets. Material and Methods: The experiments were conducted in 20 male dogs. Bands were cemented in all intermediate incisors, with conventional brackets (Morelli) on the right side and hybrid self-ligating ones (T3-American Ortho-dontics) on the left side. A 0.019? x 0.025? stainless steel wire was inserted passively in the slot of these brackets with chain elastics (250 gf) to perform sliding mechanics. Clinical records of the orthodontic mechanics were made before and after 15 days of the tooth movement. The dental segments of the animals were prepared for light micros-copy. Statistical analysis of variance and the Tukey correction with a P value at 5% were used. Results: There were no significant differences in tooth movement rates between the two types of brackets but di-fferences, in the bone modeling activity, suggested that tooth movement with the self-ligating brackets resulted in more tipping and less translational movement than tooth movement with the conventional brackets. Conclusions: The rates of tooth movement were similar between the two systems. The histological evaluation of cellular bone modeling activity along tooth root surfaces showed more translation movement of teeth with the con-ventional brackets, and more tipping movement of teeth with self-ligating brackets.
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    Immediate non-submerged implants with laser-microtextured collar placed in the inter-radicular septum of mandibular molar extraction sockets associated to GBR : results at 3-year
    (2020) Guarnieri, Renzo; Di Nardo, Dario; Di Giorgio, Gianni; Miccoli, Gabriele; Testarelli, Luca
    Background: The aim of the present study was to radiographically evaluate the vertical socket walls changes, and the peri-implant marginal bone remodelling, and clinicallly the soft tissues conditions around the non-submerged single implants placed into the inter radicular septum of mandibular molar sockets, associated with a collagen membrane, after 3 years of loading.Material and Methods: Thirty patients underwent to placement of a non-submerged implants with a laser-micro-textured collar into the inter radicular septum of mandibular molar fresh extraction sockets. A collagen membrane and the mucoperiosteal flap were adapted around the neck of the implants, leaving the laser-microtextured collar to heal in a transmucosal fashion. Results: At the end of the follow-up period, no statistical differences were found for each radiographic measure-ments used for the examination of extraction sockets vertical bone changes. Compared to implants placement, at the end of the 3-year follow-up, the vertical radiographic mesial and distal peri-implant marginal bone levels showed a statistically significant gain of 0.9 (SD 0.5), and 1.0 mm (SD 0.6), respectively (P=0.037).Conclusions: in mandibular fresh extraction sockets, the method of GBR around transmucosal implants with la-ser-microtextured surface placed into the interadicular septum may be used successfully to counteract the ridge remodelling.
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    Influence of artificial aging and ZrO2 nanoparticle-reinforced repair resin on the denture repair strength
    (2020) Gad, Mohammed-Moustafa; Rahoma, Ahmed; Abualsaud, Reem; Al Thobity, Ahmad M.; Akhtar, Sultan; Siddiqui, Intisar A.; Al Harbi, Fahad A.
    Background: The purpose of this study was to evaluate the effect of aging process on the tensile strength (TS) of repaired acrylic denture base using ZrO2 nanoparticles (nano-ZrO2)-reinforced autopolymerized resin. Material and Methods: A total of 240 heat-polymerized acrylic resin specimens (n=10) were prepared and sectioned creating 2 mm-repair-gap. Autopolymerized acrylic resin, pure and modified with 2.5, 5, and 7.5wt% nano-ZrO2were used for specimens repair. TS of repaired specimens were measured using the universal testing machine af-ter water immersion at 37oC for 2, 7 and 30 days. At each time interval, half the immersed specimens underwent thermo-cycling aging process (5000 cycles at 5/55°C) before TS testing. One-way ANOVA and Tukey-Kramer multiple-comparison tests were used for data analysis at ?=0.05. Results: Aging process for all groups showed significant differences in TS between unreinforced and nano-ZrO2reinforced groups (p<0.05). Within immersed nano-ZrO2-reinforced specimens, 5% group immersed for 30-days showed the highest significant TS value (p<0.05). With regards to thermocycling, 5% group showed the highest TS values after 2-days and 30-days groups while after 7-days, significant differences were found between 2.5% group and 5% and 7.5% groups (p?0.05). SEM images analysis displayed the ductile fracture type for nano-ZrO2reinforced groups.Conclusions: In summary, 5.0%-nano-ZrO2 addition to repair resin showed an improvement in tensile strength of repaired acrylic resin with different aging processes.
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    RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib : a randomized clinical trialy
    (2020) Medeiros Albuquerque, Assis Filipe; Sá Roriz Fonteles, Cristiane; do Nascimento Costa, José Jackson; Silva, José Roberto Viana; Silva, Paulo-Goberlânio-de Barros; Soares, Eduardo Costa Studart; Chaves, Filipe Nobre; Alves Pereira, Karuza Maria; Rodrigues-Ribeiro, Thyciana; Gurgel Costa, Fábio Wildson
    Background: This study aimed to evaluate the gene expression of cyclooxygenases (COXs) in an oral model of preemptive analgesia. Material and Methods: Gingival tissue was collected during extraction of lower third molars from a randomized, triple-blind, split-mouth and placebo-controlled study. The eligible patients were randomly sorted to receive a single dose either of ibuprofen 400mg, or etoricoxib 120 mg or a placebo, one hour prior to surgery. The temporal course of RNAm was evaluated for COX-1 and -2 by means of a quantitative polymerase chain reaction in real time (RT-qPCR) at time zero and 30 minutes after the surgical procedure began, and it was correlated with clinical parameters (pain and maximum mouth opening). Results: There was a significant increase in COX-1 expression between T0 and T30 in ibuprofen (p=0.004) and eto-ricoxib (p=0.010) groups. As regards COX-2, there were increases from T0 to T30 in all groups (placebo, p=0.012; ibuprofen, p<0.001; etoricoxib, p<0.001). All groups showed a significant decrease in COX-2:COX-1 ratio from T0 to T30 (placebo, p=0.013; ibuprofen, p<0.001; etoricoxib, p=0.047). Experimental groups showed a significant correlation between COX-1 and COX-2 levels and clinical pain parameters. Conclusions: The present preemptive analgesia study concludes that COX-2 RNAm induction was directly linked to third molar-related tissue inflammation and that the relation between COX-1 and COX-2 levels were inversely proportional to the preemptively administered nonsteroidal anti-inflammatory drugs COX-2 selectivity.
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    Influence of different thermopolymerization methods on composite resin microhardness
    (2020) Dias, Marlon-Ferreira; Espíndola Castro, Luís Felipe; Lins-Filho, Paulo-Cardoso; Teixeira, Hilcia Mezzalira; Silva, Claudio Heliomar Vicente; Guimarães, Renata-Pedrosa
    Background: Additional heat polymerization in composite resins allows greater effective ness of microhardness, flexural strength, fracture tough ness, wear resistance, and increased color stability.Material and Methods: 150 composite resin specimens were made using a 4 mm diameter and 2 mm thick bipartite steel matrix. Five resins composed of different compositions were tested (Brilliant Everglow/Coltene, Filtek One BulkFill/3M, Filtek P60/3M, Filtek Z350XT/3M, Filtek Z250XT/3M), and for each of them three types of polyme-rization were tested: light curing only (n=50); photopolymerization + autoclave thermopolymerization (n=50) and photopolymerization + microwave thermopolymerization (n=50). Each specimen was submitted to three indenta-tions by means of the Vickers microhardness test, applying a load of 300gf, associated with the time of 15s. Data were analyzed descriptively by means of statistics, standard deviation and coefficient of variation and inferentially by the F test (ANOVA) in the comparison between groups. The margin of error used in statistical test decisions was 5%.Results: The highest vicker microhardness averages were from the Control group (light curing only) on P60 (82.16) and Z250 XT (79.61) resins. The lowest averages were all verified on Brilliant Everglow resin in all polymerization methods studied: Photopolymerization (37.32), with microwave (43.80) and autoclave (45.12), followed by Bulk Fill 3M resin, ranged from 52.23 to 59.15.Conclusions: Both autoclave and microwave thermopolymerization methods showed similar behavior on the mi-crohardness of the composites studied. Considering the resin type, there was a varied behavior compared to thermo-polymerization, which increased the microhardness values for Brilliant Everglow resins (Coltene) and Filtek One Bulkfill (3M) and decreased for Filtek P60, Filtek Z350XT and Filtek Z250XT resins.
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    Understanding the role of osteoarthrosis on electromyographic activity of masticatory muscles and quality of life
    (2020) Righetti, Mariah; Taube, Oswaldo; Palinkas, Marcelo; Gonçalves, Ligia; Rufato, Fernanda; Arnoni, Veridiana; da Silva, Nayara; Regalo, Simone; Siéssere, Selma
    Background: Osteoarthrosis is a severe, evolutionary, chronic, and limiting disease that influences on quality of life, as it affects synovial joints and promoted degradation of hyaline articular cartilage. We sought to determine if electromyographic activity of the masticatory muscles and quality of life are negatively impacted by osteoarthrosis.Material and Methods: A sample of 72 participants between 40 and 70 years old diagnosed with osteoarthrosis were selected. Forty-eight participants met the inclusion criteria of this study and were divided into two distinct groups: with osteoarthrosis (n=24) and without osteoarthrosis (n=24). Electromyographic activity of the masseter and temporalis muscles (mandibular rest, right laterality, left laterality, protrusion, and dental clenching in maximal voluntary contraction) and quality of life measurements were used. Electromyographic activity was used to analyze muscle activation patterns. OHIP-14Br and SF-36 questionnaires determined the quality of life.Results: The participants with osteoarthrosis presented significant greater electromyographic activity (p?0.05) at rest for the right temporal muscle (p=0.04), maximum voluntary contraction for the left masseter muscle (P=0.04), repercussions of oral conditions on quality of life by the sum of OHIP-14 (p=0.002), and a statistically significant difference was found in all subgroups of the SF-36 scale between the subjects with and without osteoarthrosis (p?0.001).Conclusions: People with osteoarthrosis have changes in electromyographic activity of the masticatory muscles and quality of life compared to healthy participants.
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    Comparison of the facial profile attractiveness in Class III borderline patients after surgical or compensatory orthodontic treatment
    (2020) Watanabe, Julie Heide Miyazaki; Fitarelli, Francisco; de Freitas, Daniel Salvatore; Cançado, Rodrigo-Hermont; de Oliveira, Renata Cristina Gobbi; de Oliveira, Ricardo Cesar Gobbi; Valarelli, Fabricio-Pinelli; Freitas, Karina-Maria-Salvatore
    Background: This study aimed to compare the facial profile attractiveness of Class III borderline patients after surgical or compensatory orthodontic treatment. Material and Methods: The sample consisted of 60 borderline Class III malocclusion patients, divided into two groups: Group 1 (Surgical): 30 patients (16 male; 14 female) treated with orthodontic fixed appliances and bimaxi-llary orthognathic surgery. Mean initial age was 20.05 years (s.d.=2.40) and mean treatment time was 2.23 years (s.d.=0.82). Group 2 (Compensatory): 30 patients (13 male; 17 female) treated compensatorily with fixed applian-ces and Class III elastics. Mean initial age was 18.53 years (s.d.=4.35) and mean treatment time was 2.08 years (s.d.=0.67). Silhouettes of the facial profile were constructed obtained from the pretreatment and posttreatment lateral cephalograms and evaluated by orthodontists (N=41, 22 females and 19 males, mean age of 35.65 years), assigning scores from 1 (least attractive) to 10 (most attractive). Intergroup comparison of profile attractiveness was performed by Mann-Whitney test. For intragroup comparison of initial and final stages, the Wilcoxon test was used. Results: At initial stage, the compensatory group presented a statistically significant greater attractiveness of the profile than the surgical group. With treatment, the surgical group presented significantly more improvement in facial profile than the compensatory group. At the final stage, profile attractiveness of surgical and compensatory groups was similar. Conclusions: The facial profile attractiveness is similar in Class III patients after orthognathic surgery or com-pensatory orthodontic treatment. However, surgery provided more improvement in profile attractiveness than the compensatory treatment in Class III patients.
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    The effect of triangular and reversed triangular flap designs to post third molar odontectomy complications (a pilot study)
    (2020) Mudjono, Henri; Rahajoe, Poerwati Soetji; Astuti, Elizabeth Riyati Titi
    Background: Odontectomy is one of the most common surgical procedures in oral and maxillofacial surgery. Flap design influences the post operative complications. Triangular flap is the most widely used flap design but it has many shortages such as dehiscence, alveolar osteitis, reactionary bleeding, and periodontal disruption distal of second molar. The aim of this study is to introduce an alternative flap design in the surgical removal of impacted mandibular third molars ? reversed triangular flap ? and to compare this flap design with the triangular flap in case of dehiscence, reactionary bleeding, and clinical attachment loss. Material and Methods: This prospective, split-mouth study involved 15 patients with bilateral partially impacted mandibular third molars with similar impaction classification. One impacted tooth was removed using a triangular flap and the other using a reversed triangular flap. Post operative complications such as dehiscence, reactionary bleeding, and clinical attachment loss were recorded 1, 3, 7, 14, and 30 days post odontectomy. Results: Chi square test result shows that there were fewer incidences of dehiscence seven days post surgery using the reversed triangular flap (p=0.032). Mann Whitney-U test result shows that the reversed triangular flap exhibited less bleeding score on day 1 (p=0.002) and day 2 (p=0.035) post surgery. There were no statistically significant differences according to Mann Whitney-U test between the flap designs for the clinical attachment loss on distal of second molar on day 14 (p=0.512) and day 30 (p=0.902) post surgery. Conclusions: The reversed triangular flap design is preferable to triangular flap for impacted third molar surgery, escpecially in terms of wound dehisence and reactionary bleeding.
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    Resistance of bonded premolars to four artificial ageing models post enamel conditioning with a novel calcium-phosphate paste
    (2020) Ibrahim, Ali I.; Al-Hasani, Noor R.; Thompson, Van P.; Deb, Sanjukta
    Background: This in vitro study compares a novel calcium-phosphate etchant paste to conventional 37% phospho-ric acid gel for bonding metal and ceramic brackets by evaluating the shear bond strength, remnant adhesive and enamel damage following water storage, acid challenge and fatigue loading. Material and Methods: Metal and ceramic brackets were bonded to 240 extracted human premolars using two ena-mel conditioning protocols: conventional 37% phosphoric acid (PA) gel (control), and an acidic calcium-phosphate (CaP) paste. The CaP paste was prepared from ?-tricalcium phosphate and monocalcium phosphate monohydrate powders mixed with 37% phosphoric acid solution, and the resulting phase was confirmed using FTIR. The bonded premolars were exposed to four artificial ageing models to examine the shear bond strength (SBS), adhesive rem-nant index (ARI score), with stereomicroscopic evaluation of enamel damage. Results: Metal and ceramic control subgroups yielded significantly higher (p ? 0.05) SBS (17.1-31.8 MPa) than the CaP subgroups (11.4-23.8 MPa) post all artificial ageing protocols, coupled with higher ARI scores and evidence of enamel damage. In contrast, the CaP subgroups survived all artificial ageing tests by maintaining adequate SBS for clinical performance, with the advantages of leaving unblemished enamel surface and bracket failures at the enamel-adhesive interface. Conclusions: Enamel conditioning with acidic CaP pastes attained adequate bond strengths with no or minimal adhesive residue and enamel damage, suggesting a suitable alternative to the conventional PA gel for orthodontic bonding.