Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions
NAGIOS: RODERIC FUNCIONANDO

Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions

DSpace Repository

Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions

Show simple item record

dc.contributor.author Navarro-Sánchez, José
dc.contributor.author Almora-Barrios, Neyvis
dc.contributor.author Lerma-Berlanga, Belén
dc.contributor.author Ruiz Pernía, José Javier
dc.contributor.author Lórenz Fonfría, Victor Armando
dc.contributor.author Tuñón, Iñaki
dc.contributor.author Martí Gastaldo, Carlos
dc.date.accessioned 2019-05-02T14:55:56Z
dc.date.available 2019-05-02T14:55:56Z
dc.date.issued 2019
dc.identifier.uri http://hdl.handle.net/10550/70041
dc.description.abstract Enzymatic catalysis is of great importance to the chemical industry. However, we are still scratching the surface of the potential of biocatalysis due to the limited operating range of enzymes in harsh environments or their low recyclability. The role of Metal-Organic Frameworks (MOFs) as active supports to help overcome these limitations, mainly by immobilization and stabilization of enzymes, is rapidly expanding. Here we make use of mild heating and a non-polar medium during incubation to induce the translocation of a small enzyme like protease in the mesoporous MOF MIL-101(Al)-NH2. Our proteolytic tests demonstrate that protease@MIL-101(Al)-NH2 displays higher activity than the free enzyme under all the conditions explored and, more importantly, its usability can be extended to extreme conditions of pH and high temperatures. MOF immobilization is also effective in providing the biocomposite with long-term stability, recyclability and excellent compatibility with competing enzymes. This simple, one-step infiltration strategy might accelerate the discovery of new MOF-enzyme biocatalysts that meet the requirements for biotechnological applications.
dc.language.iso eng
dc.relation.ispartof Chemical Science, 2019, vol. 10, p. 4082-4088
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Navarro-Sánchez, José Almora-Barrios, Neyvis Lerma-Berlanga, Belén Ruiz Pernía, José Javier Lórenz Fonfría, Victor Armando Tuñón, Iñaki Martí Gastaldo, Carlos 2019 Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions Chemical Science 10 4082 4088
dc.subject Química organometàl·lica
dc.title Translocation of enzymes into a mesoporous MOF for enhanced catalytic activity under extreme conditions
dc.type info:eu-repo/semantics/article
dc.date.updated 2019-05-02T14:55:57Z
dc.identifier.doi https://doi.org/10.1039/C9SC00082H
dc.identifier.idgrec 131669

View       (1.453Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics