Journal of Clinical and Experimental Dentistry. 2018. Vol. 10, no. 12

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https://hdl.handle.net/10550/69420

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Effect of electric currents on antibacterial effect of chlorhexidine against Entrococcus faecalis biofilm : An in vitro study
(2018) Foroughreyhani, Mohammad; Salemmilani, Amin; Mozafari, Aysan; Soroush-Barhaghi, Mohammadhosien
This in vitro study was mainly aimed to evaluate the effect of high-frequency alternating currents (AC) applied by an electronic apex locator (EAL) on the antibacterial properties of chlorhexidine (CHX) on E. faecalis biofilm. The root canals of 120 extracted human single-rooted teeth were prepared using Gates-Glidden drills and hand K-files. After contaminating the root canals with E. faecalis, they were incubated for 60 days. Then, the teeth were randomly divided into six experimental groups (n=20). Group 1, 2% CHX; group 2, normal saline (NS) with direct current (DC); group 3, normal saline (NS) with high-frequency alternating current (AC); group 4, 2% CHX with DC; group 5, 2% CHX with AC; group 6, control (normal saline). The samples were collected from the root canal walls of 16 teeth in each group and 1:10 serial dilutions were prepared and added to Muller-Hinton agar (MHA) plates and incubated at 37°C for 48 h. The longitudinal sections of the other 4 teeth used to observe under a scanning electron microscope (SEM). A classic colony counting technique was used for counting the vital E. faecalis bacteria in MHA. Two-way ANOVA was used for statistical analysis of the data. The level of significance was set at P<0.05. The electric current significantly changed the colony-forming units (CFU) values (P<0.001). According to pair-wise comparisons, the highest CFU difference was observed between the AC group and the group without electric current (P<0.001); furthermore, the difference between the DC group and the group without electric current was not significant (P=0.823). The highest bioelectric effect occurred with the use of high-frequency alternating electric current in the form of an apex locator with CHX as a canal irrigant.
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Palatal fenestration after orthodontic treatment. Intermodal approach and evolution. A case report
(2018) Pérez-Lanza, Pablo; Almiñana Pastor, Pedro J.; Alpiste Illueca, Francisco M.
A 15-year-old patient is referred to the Department of Periodontics of the University of Valencia. The patient reported dissatisfaction with the position of upper right canine after orthodontic treatment. Previously, in a private center, surgical approaches were performed for the traction of the canine included. On clinical examination at first appointment, generalized plaque-induced gingivitis was observed, with palatal fenestration of the root of upper right lateral incisor of approximately 75% of the total surface, with suppuration and very little gingiva inserted at the cervical level, which presents a buccal torque of the crown. Radiologically, a mild rhizolysis and bone loss adjacent to upper right lateral incisor was observed. An interdisciplinary treatment is decided between the departments of Orthodontics, Endodontics and Periodontics: - Canal treatment of upper right lateral incisor, performing a retrograde filling of the cavity with Biodentine ® (Septodent, Saint Maur de Fossés, France). - Orthodontic treatment, modifying the torque and improving the stability. - Periodontal treatment, performing a connective tissue graft by Langer technique adapted to the case. After the conclusion of the orthodontic treatment, an improvement in the situation is observed. The graft was performed, presenting at 3 months a line of fenestration in the mucosa. At 4 years, the patient is asymptomatic, without suppuration, with a total closure of the fenestration.
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A comparative evaluation between dermatoglyphic patterns and different terminal planes in primary dentition
(2018) Ravindra, Vignesh; Rekha, Vishnu; Annamalai, Sankar; Sharmin, Ditto; Norouzi Baghkomeh, Parisa
To assess the correlation between different dermatoglyphic patterns with the terminal planes in deciduous dentition. 300 children who are 3-6 years old with complete primary dentition were recruited and the pattern of molar terminal plane was recorded in the proforma. Finger prints of the distal phalanges of these subjects were recorded using ink and roller method and were analysed for the finger print pattern by a forensic specialist. The pattern were classified based on classification given by Galton. The finger ridge counts were also measured. Ulnar loop pattern was the most predominant dermatoglyphic pattern. Absence of arch pattern in ring and little fingers of left hand and higher ridge count in left little finger when compared to the right hand were related to Mesial step. Presence of whorl pattern in both right and left middle finger and higher total finger ridge count in left hand when compared to the right hand were related to distal step. Flush terminal plane was related to absence of arc pattern in ring finger of left hand. Dermatoglyphics can be used as a non invasive analytical tool to predict the terminal plane in primary dentition.
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Comparison of digital protocols for the measurement of peri-implant marginal bone loss
(2018) Peñarrocha Oltra, David; Palau, Ivan; Cabanes Gumbau, Guillermo; Tarazona Álvarez, Beatriz; Peñarrocha Diago, María
The measurement of peri-implant marginal bone loss is currently carried out using digital methods of radiographic analysis assisted by various types of software. The purpose of this study was to compare the characteristics of three different softwares: specific radiology software for the development and visualization of radiological images in DICOM format (3Dicom Viewer®), advanced level software for professional editing of bitmap images (or raster graphics) (Adobe Photoshop®), and mid-level software for processing bitmap-type images, programmed in Java and in the public domain (ImageJ®). It was verified that the three softwares used are valid for the measurement of peri-implant marginal bone loss provided that the appropriate protocol is fulfilled. The results showed no significant differences between Adobe Photoshop® and ImageJ® with respect to 3Dicom Viewer® in the measurements of mesial and distal bone loss of the implants, without influence of the dental sector where they were located. The measurements made with ImageJ® looked more like those of the control software (3Dicom Viewer®) than those of Adobe Photoshop®, but with a greater degree of dispersion. Thus, Adobe Photoshop® is a slightly inaccurate method but with less dispersion.
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Pigmented odontogenic keratocyst : report of a rare case and review of the literature
(2018) Rodrigues-Fernandes, Carla-Isabelly; Bonan, Paulo Rogério Ferreti; Carvalho, Elaine Judite de Amorim; Sánchez-Romero, Celeste; Almeida, Oslei Paes de; Perez, Danyel Elias da Cruz
Pigmented odontogenic keratocyst (OKC) is very rare and its etiology remains uncertain. To the best of our knowledge, only 9 cases of pigmented OKC have been published in English-language literature. This report describes a pigmented OKC in a 14-year-old black male patient. Radiographically, the lesion appeared as a well-circumscribed, unilocular, and radiolucent image. A surgical excision was performed. Histopathological examination revealed an OKC. Additionally, a brownish, sparsed, intracytoplasmic pigmentation was observed in the basal cell layer, which was positive for Fontana-Masson staining. Immunohistochemistry reactions revealed positive dendritic cells for S-100 protein, HMB45 and Melan A. No clinical and imaging signs of recurrence were observed after 24 months. In conclusion, melanin apparently does not represent a factor for distinct biological behavior in OKC.
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Multidisciplinary oral rehabilitation in partially edentulous adult patients with malocclusion : a cross-sectional survey study
(2018) Devita, Renan; Pinho, Sérgio; Ustrell Torrent, Josep Mª; Pretti, Enrique; França, Esdras-de Campos; Silva, Ertty; Brum, Igor
A cross-sectional survey was conducted to gather information regarding the opinion of Brazilian specialists in both orthodontics and implantology on multidisciplinary oral rehabilitation in partially edentulous patients with malocclusion. A total of 305 specialists participated in a telephone survey and answered an ad hoc 10-item questionnaire, including the request of total skull cone-beam tomographies (CBCT) and the use of 3D digital planning software, the best moment of treatment to place dental implants, and the integration of orthodontics in implantology. Most participants did not request CBCT (90.8%) or 3D digital planning software images (92.3%) to diagnose and plan multidisciplinary oral rehabilitation. By contrast, 91.1% of participants would use an already dental implant as anchorage for orthodontics, 73.8% had already used implants for this purpose, 47.9% selected 4 months as the waiting time between implant placement and its use as anchorage, and 58.4% had already placed dental implants having in mind using them as anchorage for orthodontics and anticipating the oral rehabilitation process. Moreover, 93.4% of participants stated to avoid applying orthodontic forces in implants with unfavourable prognosis. A total of 67.9% of participants got the degree of specialist in Orthodontics before that of specialist in Implantology. The main reason for obtaining the other specialty degree was to be able to thoroughly exercise the two specialties. The use of technological advances, such as CBCT and 3D digital planning software was limited. Most dental specialists would wait the osseointegration recommended time before applying orthodontic forces and thus using them as anchorage for orthodontics. The majority of interviewed dentists sought the other specialty to acquire multidisciplinary knowledge.
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Root canal adaptation and intra-tubular penetration of three fiber-post cementation systems
(2018) Llena Puy, María Carmen; García-Gallart, María; Forner Navarro, Leopoldo; Ferrari, Marco
To measure the penetration of three bonding systems for the luting of fiber glass posts in endodontically treated teeth, using confocal laser scanning microscopy (CLSM). A total of 30 maxillary incisors were shaped with the Mtwo system and filled with gutta-percha and Top Seal cement. The sample was divided into three groups (ni=10) according to the bonding system used to cement the posts: Group 1 (Prime&Bond NT and Rebilda DC with a total-etch technique); Group 2 (Futurabond DC and Rebilda DC with a dentin self-etch technique); and Group 3 (BisCem self-adhesive cement). Rhodamine B was added to the bonding systems to allow visualization by CLSM. Three 1-mm thick cross-sections were obtained of each root at a distance of 2, 5 and 8 mm from the coronal limit of the root. The specific software of the CLSM system was used to measure the percentage of the root canal perimeter showing penetration of the bonding system in the dentinal tubules, together with the maximum depth of penetration. Comparison between groups were made by Kruskal Wallis test, and comparison two by two groups with Mann-Whitney U-test. Depth of penetration of the resin tags, were distributed from greater to lesser depth as follows: BisCem > Prime&Bond NT > Futurabond DC. BisCem showed significantly greater penetration in the middle and apical thirds than the rest of the systems (906.14±67.42 and 699.27±76.26 µm, respectively). The percentage perimeter exhibiting penetration in the coronal third was significantly greater with BisCem versus Futurabond DC (56.08±7.24 and 44.38±5.23%, respectively). No significant differences were recorded in the middle and apical thirds among the three systems. BisCem resulted in greater depth of intratubular penetration at all studied levels. The percentage perimeter of the canal showing penetration was similar for all the bonding systems.
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A sample of non-nutritive sucking habits (pacifier and digit) in portuguese children and its relation with the molar classes of angle
(2018) Machado, Sónia-Cristina-Silva; Manzanares Céspedes, María Cristina; Ferreira-Moreira, Joaquim; Pacheco, José Júlio; Rompante, Paulo-Alexandre-Martins-Abreu; Ustrell Torrent, Josep Mª
Little is known about the effect of non-nutritive sucking habits (pacifier and digital sucking) in the prevalence of molar Class in mixed dentition. The aim of this study was determinate the relation between non-nutritive sucking habits, and Angle´s molar Class, in the horizontal plane, and it´s relation with gender. A convenience sample of 326 children with ages between 6 and 12 years was selected from three schools of Oporto. To collect the epidemiologic data, was used a method recommended by the WHO. An indirect questionnaire about the medical history, dental habits, was used. It was adapted from Sanchez-Molins and validated by Clinical Dental III of Integrated Dental University Institute Health Sciences, Gandra, Portugal. In this study, 326 infants were examined in order to determine the prevalence of non-nutritive sucking habits. Only 45 observed children did not mentioned any kind of non-nutritive sucking habit; the remaining 281 children mentioned at least one potential bad habit. Children with non-nutritive sucking habits show a higher molar Class II percentage in females, while molar Class III is more frequent among males compared with children with no sucking habits. Children with non-nutritive sucking habits, presented a higher-Class II prevalence with statistically significance. It was detected a direct relationship between Angle´s molar Class and gender.
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Dentist´s knowledge, attitudes and determining factors of the conservative approach in teeth with reversible pulpitis and deep caries lesions
(2018) Crespo-Gallardo, Isabel; Martín González, Jenifer; Jiménez Sánchez, María del Carmen; Cabanillas-Balsera, Daniel; Sánchez Domínguez, Benito; Segura-Egea, Juan J.
The aim of this study was to investigate dentists` knowledge, attitudes and factors regarding the conservative approach in the management of deep caries lesions (DCLs) in teeth with reversible pulpitis. 187 dentists were contacted directly or by mail, and 125 (67%) were finally included in the study. Dentists were requested to answer an open/discursive questionnaire about the routine approach to the diagnosis and treatment of DCLs, including knowledge-related attitudinal items. Total caries excavation was the preferred treatment option for more than 80% of dentists in case of DCL with reversible pulpitis. Only a small percentage (8%) chose partial caries removal, leaving some carious dentin close to the pulp to avoid pulp exposure. More than a half (51%) of respondents considered that cariogenic microorganisms must be removed or caries would progress. Dentists teaching at the University strongly disagreed with this statement (OR = 4.6; 95% C.I. = 1.3 ? 15.8; p = 0.017). Good clinical result was the most chosen reason (83%) to choose a specific treatment. Patient?s oral health (84%) and patient?s age (70%) were the two patient-related factors most taken into account for the choice of treatment. Total caries excavation is still the most frequently chosen treatment in teeth with DCL and reversible pulpitis. The joint assessment of the answers given by respondents allows to conclude that the new knowledge and concepts about caries lesions and the more conservative approach to DCLs have not still been incorporated by dentists into their usual clinical practice.
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Transcultural adaptation and validation of the Spanish language version of the questionnaire OQLQ for the assessment of quality of life in orthognathic patients
(2018) Sánchez Burgos, R.; Martínez Gimeno, Carlos; Arribas-García, Ignacio; Gómez Oliveira, G.; Álvarez Flores, Modesto; García Hernández, Alberto; Martínez-Martínez, Ricardo
Orthognathic surgery is the cornerstone of the treatment of dentofacial deformities, which have a great psychological and social impact on the life of the patient. Patient satisfaction and the impact on quality of life have recently become clinical parameters of growing importance. The aim of this study was to undertake a transcultural adaptation, translation to Spanish and validation of this version of the questionnaire OQLQ, used to measure quality of life in the context of Spanish culture. Validation of the OQLQ questionnaire to the Spanish language was carried out through the methodology of translation and back translation, conceptual equivalence and piloting. The Spanish version was applied through a cross-sectional study to a total of 50 patients undergoing orthognathic surgery. The adapted and validated version showed adequate metric properties of reliability, change sensitivity and validity. In this study, a positive impact of orthognathic surgery on the specific quality of life was evident in 96% of patients, with an average improvement of 58% with respect to the initial score. Dentofacial deformities have a marked negative impact on the lives of patients, with orthognathic surgery being a therapeutic tool of great value in improving the quality of life in social, functional and aesthetic terms. The pilot test of this Spanish language version of the OQLQ proved valid for the assessment of quality of life in Spanish-speaking orthognathic patients or those with a Spanish culture.
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Comparative evaluation of antibacterial effect of nanoparticles and lasers against Endodontic Microbiota : an in vitro study
(2018) Kushwaha, Vibha; Yadav, Rakesh-Kumar; Prakash Tikku, Aseem; Chandra, Anil; Verma, Promila; Gupta, Prashant; Kumar Shakya, Vijay
Present study was conducted with the aim of evaluating antimicrobial efficacy of silver (AgNP) and gold nanoparticles (AuNP) with and without Nd: YAG laser (L) irradiation against experimentally inoculated Enterococcus faecalis in infected human root dentin. 120 extracted single rooted human teeth were prepared and inoculated with E. faecalis for 24 hrs. The teeth were then randomly divided into 4 experimental group: AgNPs group: irrigation for 3minutes with 50 ?l of 100 ppm, the AuNPs group: irrigation with 50 ?l of 100 ppm, the AgNPs & Nd: YAG lasers group: irrigation with 50 ?l of 100 ppm + irradiation with 1.5W laser for 60 seconds, the AuNPs & Nd: YAG lasers group: irrigation with 50 ?l of 100 ppm + irradiation with 1.5W laser for 60 seconds. One control group consisting of 2% CHX irrigation for 3 minutes was also there (n = 20). The specimens were collected from the canal before and after irrigation, and colony forming units were observed. Significant difference was found among all the groups in comparison to the control group (p<0.05). The greatest reduction in CFU?s was observed with combination of AgNPs & Nd: YAG lasers group. AgNPs in combination with Nd: YAG laser irradiation has the potential to be used as root canal disinfectant.
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Convergence angles of all-ceramic full crown preparations performed in Dubai private practice
(2018) Abdulla, Fahad; Khamis, Hassan; Milosevic, Alexander; Abuzayda, Moosa
This study aimed to determine the degree of taper and total occlusal convergence angles (TOC) for all-ceramic bonded crown preparations carried out by private practitioners in Dubai, UAE. A convenience sample of all-ceramic crown preparations carried out by private dental practitioners were scanned (Carestream CS 3500) from casts and the digital images assessed. The degree of taper was measured on the axial walls of each crown preparation and the bucco-lingual and mesio-distal convergence angles subsequently calculated. A total of 154 dentists prepared a total of 206 crown preparations (72 anterior, 134 posterior). The mean convergence angles mesio-distally for all preparations was 24.6° (sd 11.8º), and for the bucco-lingual it was 32.6° (sd 15.3°). The mean TOC was 28.6°. In anterior preparations, the mean bucco-lingual convergence angle was 38.8° (sd 12.2°) compared to 29.3° (sd 15.5°) for posterior preparations (p<0.001). Mean mesio-distal convergence anteriorly was 20.6° (sd 10.18°) compared to 26.7° (sd 12.16°) posteriorly (p<0.001). Distal and buccal taper were significantly greater on posterior teeth (<0.001) compared to anteriors whereas lingual taper was greater on anterior teeth (p<0.001). Mesial taper was not different. Premolars had significantly lower convergence values compared to other teeth. Bucco-lingual and mesio-distal convergence angles significantly exceeded the clinically acceptable convergence angle of between 10° and 22°. Greater axial taper is recommended for resin bonded all-ceramic crowns but reliance on adhesion in such preparations rather than parallelism may reduce retention and have increased biologic cost to pulp health.
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Use of bone scintigraphy in the early diagnosis of bisphosphonate related osteonecrosis of the jaw. Case report and review of the literature
(2018) Sánchez López, José Darío; Cariati, Paolo; Cambil Martín, Jacobo; Villegas Calvo, Mercedes; Moreno-Martin, María-Luisa
The main aim of the present report is to show the potential utility of bone scintigraphy for the diagnosis of jaw osteonecrosis. We report the history of a 62-year-old woman underwent breast cancer surgery in 2010. Moreover, patient received postoperative radiotherapy and chemotherapy. Intravenous bisphosphonates were also added to the treatment strategy to reduce the risk of bone metastasis. However, a hypermetabolic focus on left hemimandible was evidenced with a bone scintigraphy during follow up. After a careful study, the diagnosis of Bisphosphonate Related Ostneonecrosis of the Jaw (BRONJ) was carried out. This case highlights that bone scintigraphy may be extremely helpful for the early detection of BRONJ in high risk patient.
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Shear bond strength of ceramic bracket bonded to different surface-treated ceramic materials
(2018) Juntavee, Niwut; Juntavee, Apa; Wongnara, Krittaphat; Klomklorm, Pimkhwan; Khechonnan, Ronnaphum
This study evaluated the effect of ceramic surface treatments on bond strength of ceramic brackets to machine-able ceramics and ceramic veneering metal. Machined ceramic specimens (10x10x1.5 mm) were prepared from Empress® CAD (EP), and e.max® CAD (EM). Ceramic veneering metal specimens (PF) were fabricated from sintered d.Sign® porcelain (1.27 mm thickness) over d.Sign®10 metal (0.23 mm thickness). Each ceramic was divided into 3-groups and treated surface by Er-YAG laser (LE) or etching with 9.6% HF acid for 5 seconds (A5) or 15 seconds (A15). Resin adhesive (Transbond?-XT) was used for attaching ceramic brackets for each group (n=15) and cured with LED (Bluephase®) for 50 seconds. Specimens were immersed in distilled water for 24 hours before testing for shear bond at crosshead speed of 1.0 mm/min. The data were analyzed for the differences in bond strength with ANOVA and Tukey?s multiple comparisons (? = 0.05). De-bond surfaces were microscopically examined. Bond strength (MPa) were 12.65±1.14 for EMA5, 14.50±2.21 for EMA15, 13.97±1.17 for EMLE, 12.40±1.95 for PFA5, 15.85±3.13 for PFA15, 14.06±2.17 for PFLE, 12.12±1.54 for EPA5, 15.65±1.57 for EPA15, 12.89±1.17 for EPLE. Significant differences in bond strength among groups were found related to surface treatment (p<0.05), but not significant difference upon type of ceramics (p>0.05). A15 provided higher bond strength than LE and A5 (P<0.05). No damage of ceramic surface upon de-bonding was indicated except for A15 tends to exhibit ditching. LE showed more uniform treated surface for bonding and no surface destruction upon de-bond compared to others. Bond strength was affected by surface treatment. Both LE and A15 treated surface provided higher bond strength than A5. Considering possibly inducing defect on ceramic surface, LE seems to provide better favorable surface preparation than others. Treated ceramic surface with Er-YAG prior to bracket bonding is recommended.
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Aplasia of the lacrimal and major salivary glands (ALSG). First case report in spanish population and review of the literature
(2018) Neagu, David; Patiño Seijas, Beatriz; Luaces Rey, Ramón; Collado-López, Javier; García-Rozado González, Alvaro; López-Cedrún Cembranos, José Luis
Aplasia of the lacrimal and the major salivary glands (ALSG) is a rare disorder with scarce cases described in the recent literature. The pattern of genetic inheritance is autosomal dominant with variable expressivity. A 40 years male patient was referred to the Oral and Maxillofacial Service at the Hospital Universitario de A Coruña diagnosed with complete agenesis of all salivary glands. Our case it is the first of ALSG syndrome in the Spanish literature. Imaging tests are necessary to confirm the lack of formation of salivary glands and alteration of lacrimal system. A mutation of FGF10 has been proposed as the responsible of the syndrome. The management of the lacrimal alteration depends of the clinical findings. Clinical suspicion remains the principal tool to diagnose the syndrome.

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