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A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase

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A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase

dc.contributor.author	Blanco-Aparicio, Carmen
dc.contributor.author	Torres Ibáñez, José Manuel
dc.contributor.author	Pulido Murillo, Rafael
dc.date.accessioned	2022-11-03T14:27:04Z
dc.date.available	2022-11-03T14:27:04Z
dc.date.issued	1999
dc.identifier.uri	https://hdl.handle.net/10550/84390
dc.description.abstract	Protein tyrosine phosphatase PTP-SL retains mitogen-activated protein (MAP) kinases in the cytoplasm in an inactive form by association through a kinase interaction motif (KIM) and tyrosine dephosphorylation. The related tyrosine phosphatases PTP-SL and STEP were phosphorylated by the cAMP-dependent protein kinase A (PKA). The PKA phosphorylation site on PTP-SL was identified as the Ser231 residue, located within the KIM. Upon phosphorylation of Ser231, PTP-SL binding and tyrosine dephosphorylation of the MAP kinases extracellular signal-regulated kinase (ERK)1/2 and p38α were impaired. Furthermore, treatment of COS-7 cells with PKA activators, or overexpression of the Cα catalytic subunit of PKA, inhibited the cytoplasmic retention of ERK2 and p38α by wild-type PTP-SL, but not by a PTP-SL S231A mutant. These findings support the existence of a novel mechanism by which PKA may regulate the activation and translocation to the nucleus of MAP kinases.
dc.language.iso	eng
dc.relation.ispartof	Journal of Cell Biology, 1999, vol. 147, num. 6, p. 1129-1136
dc.source	Blanco-Aparicio, Carmen Torres Ibáñez, José Manuel Pulido Murillo, Rafael 1999 A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase Journal of Cell Biology 147 6 1129 1136
dc.subject	Cèl·lules
dc.subject	Enzims
dc.title	A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase
dc.type	journal article	es_ES
dc.date.updated	2022-11-03T14:27:04Z
dc.identifier.doi	https://doi.org/10.1083/jcb.147.6.1129
dc.identifier.idgrec	080762
dc.rights.accessRights	open access	es_ES