Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes
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Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes

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Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes

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dc.contributor.author Guijarro Ferrer, Lluís
dc.contributor.author Martínez Camarena, Álvaro
dc.contributor.author Chicote, Javier U.
dc.contributor.author García-España, Antonio
dc.contributor.author Garcia-España Monsonis, Enrique
dc.contributor.author Inclán Nafría, Mario Alfonso
dc.contributor.author Verdejo Viu, Begoña
dc.contributor.author González García, Jorge
dc.date.accessioned 2022-04-29T13:56:40Z
dc.date.available 2022-04-29T13:56:40Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/10550/82471
dc.description.abstract Nucleic acids are essential biomolecules in living systems and represent one of the main targets of chemists, biophysics, biologists, and nanotechnologists. New small molecules are continuously developed to target the duplex (ds) structure of DNA and, most recently, RNA to be used as therapeutics and/or biological tools. Stimuli-triggered systems can promote and hamper the interaction to biomolecules through external stimuli such as light and metal coordination. In this work, we report on the interaction with ds-DNA and ds-RNA of two aza-macrocycles able to coordinate Zn2+ metal ions and form binuclear complexes. The interaction of the aza-macrocycles and the Zn2+ metal complexes with duplex DNA and RNA was studied using UV thermal and fluorescence indicator displacement assays in combination with theoretical studies. Both ligands show a high affinity for ds-DNA/RNA and selectivity for ds-RNA. The ability to interact with these duplexes is blocked upon Zn2+ coordination, which was confirmed by the low variation in the melting temperature and poor displacement of the fluorescent dye from the ds-DNA/RNA. Cell viability assays show a decrease in the cytotoxicity of the metal complexes in comparison with the free ligands, which can be associated with the observed binding to the nucleic acids.
dc.language.iso eng
dc.relation.ispartof Molecules, 2021, vol. 26, num. 13, p. 3957
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Guijarro Ferrer, Lluís Martínez Camarena, Álvaro Chicote, Javier U. García-España, Antonio Garcia-España Monsonis, Enrique Inclán Nafría, Mario Alfonso Verdejo Viu, Begoña González García, Jorge 2021 Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes Molecules 26 13 3957
dc.subject RNA
dc.subject Àcids nucleics
dc.title Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes
dc.type info:eu-repo/semantics/article
dc.date.updated 2022-04-29T13:56:41Z
dc.identifier.doi https://doi.org/10.3390/molecules26133957
dc.identifier.idgrec 152082

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