Ethanol impairs extracellular zinc intake in cultured astrocytes

Ethanol impairs extracellular zinc intake in cultured astrocytes

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Ethanol impairs extracellular zinc intake in cultured astrocytes

Show simple item record Ponsoda i Martí, Xavier Ballestín Hinojosa, Raúl Molowny Tudela, Asunción Marín Muela, María Pilar Esteban Pretel, Guillermo Romero, Ana Renau Piqueras, Jaime López García, Carlos 2022-04-07T11:43:41Z 2022-04-08T04:45:05Z 2009 es_ES
dc.description.abstract Zinc (Zn) deficiency is present in many physiological and health problems. Among circumstances involved in Zn deficiency, ethanol consumption appears as a prominent cause. In the CNS substantial amounts of Zn appear accumulated in synaptic vesicles of a particular class of neurons: the Zn enriched neurons very abundant in the telencephalon and cerebral cortex. This is the so called synaptic Zn which is simultaneously released with the neurotransmitter thus exerting a neuromodulator role during synaptic transmission. Neighbour astrocytic processes have to capture the excess of both extracellular Zn and neurotransmitter in order to maintain efficient synaptic transmission between neurons. In this work we analyze the effect of exposure to 30 mM ethanol for 7 days in the ability of cultured rat astrocytes to capture and manage extracellular Zn. Intracellular Zn levels were visualized by using the TSQ Zn fluorochrome, either in normal culture conditions or after supplementary addition of 50 μM ZnSO4 to the culture. Fluorescence was recorded with an Olympus microscope BX50WI, equipped with a Hamamatsu ORCA digital camera controlled with the Aquacosmos software. Basal Zn levels in cultured astrocytes was greatly and significantly lower in ethanol treated cells (about 30% of control cultures). These differences were consistently maintained after addition of extracellular Zn to cell monolayers, resulting in a lower ability to uptake or retain Zn. The Zn was uptaked by the endocytic pathway, as demonstrated by the marker FM1-43 and was mainly confined to bright organelles that were more abundant in control cells. In conclusion, ethanol impairs astrocyte Zn management resulting in a lower capacity for extracellular Zn intake in resting conditions and after extracellular addition. It has been proposed that an efficient method to palliate Zn deficiency it could be a dietary supplement. Nevertheless, this study suggests that a dietary Zn supplementation may not be enough for recovery of cellular normal function in alcoholic cultured astrocytes. es_ES
dc.language.iso es es_ES
dc.source Comunicación 2009-09-16 XIII Congreso Sociedad Española Neurociencia TARRAGONA es_ES
dc.subject etanol es_ES
dc.subject zinc es_ES
dc.subject astrocito es_ES
dc.subject cultivo celular es_ES
dc.title Ethanol impairs extracellular zinc intake in cultured astrocytes es_ES
dc.type info:eu-repo/semantics/conferenceObject es_ES
dc.subject.unesco UNESCO::PSICOLOGÍA es_ES
dc.accrualMethod - es_ES
dc.embargo.terms 0 days es_ES

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