Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast
NAGIOS: RODERIC FUNCIONANDO

Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast

DSpace Repository

Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast

Show simple item record

dc.contributor.author Pérez Ortín, José Enrique
dc.contributor.author Mena, Adriana
dc.contributor.author Barba-Aliaga, Marina
dc.contributor.author Singh, Abhyudai
dc.contributor.author Chávez, Sebastián
dc.contributor.author García Martínez, José
dc.date.accessioned 2021-07-21T13:59:24Z
dc.date.available 2021-07-21T13:59:24Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/10550/79999
dc.description.abstract The adjustment of transcription and translation rates to the changing needs of cells is of utmost importance for their fitness and survival. We have previously shown that the global transcription rate for RNA polymerase II in budding yeast Saccharomyces cerevisiae is regulated in relation to cell volume. Total mRNA concentration is constant with cell volume since global RNApol II-dependent nascent transcription rate (nTR) also keeps constant but mRNA stability increases with cell size. In this paper, we focus on the case of rRNA and RNA polymerase I. Contrarily to that found for RNA pol II, we detected that RNA polymerase I nTR increases proportionally to genome copies and cell size in polyploid cells. In haploid mutant cells with larger cell sizes, the rDNA repeat copy number rises. By combining mathematical modeling and experimental work with the large-size cln3 strain, we observed that the increasing repeat copy number is based on a feedback mechanism in which Sir2 histone deacetylase homeostatically controls the amplification of rDNA repeats in a volume-dependent manner. This amplification is paralleled with an increase in rRNA nTR, which indicates a control of the RNA pol I synthesis rate by cell volume.
dc.language.iso eng
dc.relation.ispartof Plos Genetics, 2021, vol. 17, num. 4, p. e1009520
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Pérez Ortín, José Enrique Mena, Adriana Barba-Aliaga, Marina Singh, Abhyudai Chávez, Sebastián García Martínez, José 2021 Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast Plos Genetics 17 4 e1009520
dc.subject RNA
dc.subject Cèl·lules eucariotes
dc.subject Genòmica
dc.title Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast
dc.type info:eu-repo/semantics/article
dc.date.updated 2021-07-21T13:59:25Z
dc.identifier.doi https://doi.org/10.1371/journal.pgen.1009520
dc.identifier.idgrec 144785

View       (1.755Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics