Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice
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Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

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Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice

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dc.contributor.author Rubio-Del-Campo, Antonio
dc.contributor.author Gozalbo Rovira, Roberto Vicente
dc.contributor.author Moya-Gonzálvez, Eva M.
dc.contributor.author Alberola Enguídanos, Juan
dc.contributor.author Rodríguez Díaz, Jesús
dc.contributor.author Yebra Yebra, María Jesús
dc.date.accessioned 2021-07-16T13:47:24Z
dc.date.available 2021-07-16T13:47:24Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/10550/79964
dc.description.abstract Human milk glycans present a unique diversity of structures that suggest different mechanisms by which they may affect the infant microbiome development. A humanized mouse model generated by infant fecal transplantation was utilized here to evaluate the impact of fucosyl-α1,3-GlcNAc (3FN), fucosyl-α1,6-GlcNAc, lacto-N-biose (LNB) and galacto-N-biose on the fecal microbiota and host-microbiota interactions. 16S rRNA amplicon sequencing showed that certain bacterial genera significantly increased (Ruminococcus and Oscillospira) or decreased (Eubacterium and Clostridium) in all disaccharide-supplemented groups. Interestingly, cluster analysis differentiates the consumption of fucosyl-oligosaccharides from galactosyl-oligosaccharides, highlighting the disappearance of Akkermansia genus in both fucosyl-oligosaccharides. An increment of the relative abundance of Coprococcus genus was only observed with 3FN. As well, LNB significantly increased the relative abundance of Bifidobacterium, whereas the absolute levels of this genus, as measured by quantitative real-time PCR, did not significantly increase. OTUs corresponding to the species Bifidobacterium longum, Bifidobacterium adolescentis and Ruminococcus gnavus were not present in the control after the 3-week intervention, but were shared among the donor and specific disaccharide groups, indicating that their survival is dependent on disaccharide supplementation. The 3FN-feeding group showed increased levels of butyrate and acetate in the colon, and decreased levels of serum HDL-cholesterol. 3FN also down-regulated the pro-inflammatory cytokine TNF-α and up-regulated the anti-inflammatory cytokines IL-10 and IL-13, and the Toll-like receptor 2 in the large intestine tissue. The present study revealed that the four disaccharides show efficacy in producing beneficial compositional shifts of the gut microbiota and in addition, the 3FN demonstrated physiological and immunomodulatory roles.
dc.language.iso eng
dc.relation.ispartof Gut Microbes, 2021, vol. 13, num. 1:e1914377, p. 1-20
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Rubio-Del-Campo, Antonio Gozalbo Rovira, Roberto Vicente Moya-Gonzálvez, Eva M. Alberola Enguídanos, Juan Rodríguez Díaz, Jesús Yebra Yebra, María Jesús 2021 Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice Gut Microbes 13 1:e1914377 1 20
dc.subject Microbiologia
dc.subject Infants Malalties
dc.title Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice
dc.type info:eu-repo/semantics/article
dc.date.updated 2021-07-16T13:47:24Z
dc.identifier.doi https://doi.org/10.1080/19490976.2021.1914377
dc.identifier.idgrec 147326

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