Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer
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Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

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Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

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dc.contributor.author Vieira, Rúbia Rocha es
dc.contributor.author Campos, Laura Hildebrand es
dc.contributor.author Jesus, Luciano Henrique es
dc.contributor.author Klabunde, Caroline es
dc.contributor.author Gamba, Thiago de Oliveira es
dc.contributor.author Flores, Isadora Luana es
dc.contributor.author Oliveira, Márcia Gaiger de es
dc.contributor.author Rados, Pantelis Varvaki es
dc.date.accessioned 2021-05-04T11:01:16Z
dc.date.available 2021-05-04T11:01:16Z
dc.date.issued 2020 es
dc.identifier.uri https://hdl.handle.net/10550/79078
dc.description.abstract The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up over an 8-year period. For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical expression with different variables, considering p<0.05. Kaplan-Meier curves were produced for local recurrence, regional metastasis and treatment. A mRNA overexpression of ALDH1 in primary tumors was associated with regional metastasis and a high ALDH1 immunostaining was related to metastasis and a worse patient outcome. Additionally, a favorable outcome was associated with the transition phase and an unfavorable outcome was associated with EMT event. An overall 26.9 months was observed with longer survival associated with surgery and radiotherapy. However, due to the intense variability inherent to the indicator proteins in the EMT process, the complete profile markers related to this biological process should be continuous investigated. es
dc.source Vieira, Rúbia Rocha ; Campos, Laura Hildebrand ; Jesus, Luciano Henrique ; Klabunde, Caroline ; Gamba, Thiago de Oliveira ; Flores, Isadora Luana ; Oliveira, Márcia Gaiger de ; Rados, Pantelis Varvaki. Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 25 6 2020: 4- es
dc.title Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer es
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.23777 es

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