MicroRNA-33b Suppresses Epithelial-Mesenchymal Transition Repressing the MYC-EZH2 Pathway in HER2+ Breast Carcinoma
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MicroRNA-33b Suppresses Epithelial-Mesenchymal Transition Repressing the MYC-EZH2 Pathway in HER2+ Breast Carcinoma

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MicroRNA-33b Suppresses Epithelial-Mesenchymal Transition Repressing the MYC-EZH2 Pathway in HER2+ Breast Carcinoma

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dc.contributor.author Pattanayak, Chaudhuri Birlipta
dc.contributor.author Garrido-Cano, Iris
dc.contributor.author Adam-Artigues, Anna
dc.contributor.author Tormo, Eduardo
dc.contributor.author Pineda Merlo, Begoña
dc.contributor.author Cabello, Paula
dc.contributor.author Alonso, Elisa
dc.contributor.author Bermejo, Begoña
dc.contributor.author Hernando, Cristina
dc.contributor.author Martínez Martínez, María Teresa
dc.contributor.author Rovira, Ana
dc.contributor.author Albanell, Joan
dc.contributor.author Rojo, Federico
dc.contributor.author Burgues Gasión, Octavio
dc.contributor.author Cejalvo Andújar, Juan Miguel
dc.contributor.author Lluch Hernández, Ana
dc.contributor.author Eroles Asensio, Pilar
dc.date.accessioned 2021-04-15T15:33:26Z
dc.date.available 2021-04-15T15:33:26Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/10550/78689
dc.description.abstract Downregulation of miR-33b has been documented in many types of cancers and is being involved in proliferation, migration, and epithelial-mesenchymal transition (EMT). Furthermore, the enhancer of zeste homolog 2-gene (EZH2) is a master regulator of controlling the stem cell differentiation and the cell proliferation processes. We aim to evaluate the implication of miR-33b in the EMT pathway in HER2+ breast cancer (BC) and to analyze the role of EZH2 in this process as well as the interaction between them. miR-33b is downregulated in HER2+ BC cells vs healthy controls, where EZH2 has an opposite expression in vitro and in patients' samples. The upregulation of miR-33b suppressed proliferation, induced apoptosis, reduced invasion, migration and regulated EMT by an increase of E-cadherin and a decrease of ß-catenin and vimentin. The silencing of EZH2 mimicked the impact of miR-33b overexpression. Furthermore, the inhibition of miR-33b induces cell proliferation, invasion, migration, EMT, and EZH2 expression in non-tumorigenic cells. Importantly, the Kaplan-Meier analysis showed a significant association between high miR-33b expression and better overall survival. These results suggest miR-33b as a suppressive miRNA that could inhibit tumor metastasis and invasion in HER2+ BC partly by impeding EMT through the repression of the MYC-EZH2 loop.
dc.language.iso eng
dc.relation.ispartof Frontiers In Oncology, 2020, vol. 10, p. 1661-1661
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Pattanayak, Chaudhuri Birlipta Garrido-Cano, Iris Adam-Artigues, Anna Tormo, Eduardo Pineda Merlo, Begoña Cabello, Paula Alonso, Elisa Bermejo, Begoña Hernando, Cristina Martínez Martínez, María Teresa Rovira, Ana Albanell, Joan Rojo, Federico Burgues Gasión, Octavio Cejalvo Andújar, Juan Miguel Lluch Hernández, Ana Eroles Asensio, Pilar 2020 MicroRNA-33b Suppresses Epithelial-Mesenchymal Transition Repressing the MYC-EZH2 Pathway in HER2+ Breast Carcinoma Frontiers In Oncology 10 1661 1661
dc.subject Càncer
dc.subject Mama Examen
dc.title MicroRNA-33b Suppresses Epithelial-Mesenchymal Transition Repressing the MYC-EZH2 Pathway in HER2+ Breast Carcinoma
dc.type info:eu-repo/semantics/article
dc.date.updated 2021-04-15T15:33:26Z
dc.identifier.doi https://doi.org/10.3389/fonc.2020.01661
dc.identifier.idgrec 142836

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