Interaction of intestinal bacteria with human rotavirus during infection in children
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Interaction of intestinal bacteria with human rotavirus during infection in children

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Interaction of intestinal bacteria with human rotavirus during infection in children

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dc.contributor.author Gozalbo Rovira, Roberto Vicente
dc.contributor.author Rubio-Del-Campo, Antonio
dc.contributor.author Santiso Bellón, Cristina
dc.contributor.author Vila Vicent, Susana
dc.contributor.author Buesa Gómez, Javier
dc.contributor.author Delgado, Susana
dc.contributor.author Molinero, Natalia
dc.contributor.author Margolles, Abelardo
dc.contributor.author Yebra Yebra, María Jesús
dc.contributor.author Collado, María Carmen
dc.contributor.author Monedero García, Vicente
dc.contributor.author Rodríguez Díaz, Jesús
dc.date.accessioned 2021-01-29T14:45:09Z
dc.date.available 2021-01-29T14:45:09Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/10550/77548
dc.description.abstract The gut microbiota has emerged as a key factor in the pathogenesis of intestinal viruses, including enteroviruses, noroviruses and rotaviruses (RVs), where stimulatory and inhibitory effects on infectivity have been reported. With the aim of determining whether members of the microbiota interact with RVs during infection, a combination of anti-RV antibody labeling, fluorescence-activated cell sorting and 16S rRNA amplicon sequencing was used to characterize the interaction between specific bacteria and RV in stool samples of children suffering from diarrhea produced by G1P[8] RV. The genera Ruminococcus and Oxalobacter were identified as RV binders in stools, displaying enrichments between 4.8- and 5.4-fold compared to samples nonlabeled with anti-RV antibodies. In vitro binding of the G1P[8] Wa human RV strain to two Ruminococcus gauvreauii human isolates was confirmed by fluorescence microscopy. Analysis in R. gauvreauii with antibodies directed to several histo-blood group antigens (HBGAs) indicated that these bacteria express HBGA-like substances on their surfaces, which can be the target for RV binding. Furthermore, in vitro infection of the Wa strain in differentiated Caco-2 cells was significantly reduced by incubation with R. gauvreauii. These data, together with previous findings showing a negative correlation between Ruminococcus levels and antibody titers to RV in healthy individuals, suggest a pivotal interaction between this bacterial group and human RV. These results reveal likely mechanisms of how specific bacterial taxa of the intestinal microbiota could negatively affect RV infection and open new possibilities for antiviral strategies.
dc.language.iso eng
dc.relation.ispartof International Journal Of Molecular Sciences, 2021, vol. 22, num. 3, p. 1010
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Gozalbo Rovira, Roberto Vicente Rubio-Del-Campo, Antonio Santiso Bellón, Cristina Vila Vicent, Susana Buesa Gómez, Javier Delgado, Susana Molinero, Natalia Margolles, Abelardo Yebra Yebra, María Jesús Collado, María Carmen Monedero García, Vicente Rodríguez Díaz, Jesús 2021 Interaction of intestinal bacteria with human rotavirus during infection in children International Journal Of Molecular Sciences 22 3 1010
dc.subject Microbiologia
dc.subject Bacteris
dc.title Interaction of intestinal bacteria with human rotavirus during infection in children
dc.type info:eu-repo/semantics/article
dc.date.updated 2021-01-29T14:45:10Z
dc.identifier.doi https://doi.org/10.3390/ijms22031010
dc.identifier.idgrec 143008

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