BIO Bragg gratings on microfibers for label-free biosensing
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BIO Bragg gratings on microfibers for label-free biosensing

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BIO Bragg gratings on microfibers for label-free biosensing

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dc.contributor.author Juste-Dolz, Augusto
dc.contributor.author Delgado-Pinar, Martina
dc.contributor.author Avellà-Oliver, Miquel
dc.contributor.author Fernández, Estrella
dc.contributor.author Pastor, Daniel
dc.date.accessioned 2021-01-25T15:50:08Z
dc.date.available 2021-01-25T15:50:08Z
dc.date.issued 2021 es_ES
dc.identifier.uri https://hdl.handle.net/10550/77527
dc.description.abstract Discovering nanoscale phenomena to sense biorecognition events introduces new perspectives to exploit nanoscience and nanotechnology for bioanalytical purposes. Here we present Bio Bragg Gratings (BBGs), a novel biosensing approach that consists of diffractive structures of protein bioreceptors patterned on the surface of optical waveguides, and tailored to transduce the magnitude of biorecognition assays into the intensity of single peaks in the reflection spectrum. This work addresses the design, fabrication, and optimization of this system by both theoretical and experimental studies to explore the fundamental physicochemical parameters involved. Functional biomolecular gratings are fabricated by microcontact printing on the surface of tapered optical microfibers, and their structural features were characterized. The transduction principle is experimentally demonstrated, and its quantitative bioanalytical prospects are assessed in a representative immunoassay, based on patterned protein probes and selective IgG targets, in label-free conditions. This biosensing system involves appealing perspectives to avoid unwanted signal contributions from non-specific binding, herein investigated in human serum samples. The work also proves how the optical response of the system can be easily tuned, and it provides insights into the relevance of this feature to conceive multiplexed BBG systems capable to perform multiple label-free biorecognition assays in a single device. es_ES
dc.description.sponsorship CTQ2016-75749-R es_ES
dc.description.sponsorship TEC2016-80385-P es_ES
dc.description.sponsorship PID2019-110877 GB-I00 es_ES
dc.description.sponsorship PDI2019- 104276RB-I00 es_ES
dc.description.sponsorship PROMETEO/2019/048 es_ES
dc.description.sponsorship PROMETEO/2017/103 es_ES
dc.language.iso en es_ES
dc.source A. Juste-Dolz, M. Delgado-Pinar, M. Avella-Oliver, E. Fernández, D. Pastor, M. V. Andrés, Á. Maquieira, "BIO Bragg gratings on microfibers for label-free biosensing", Biosensors & Bioelectronics, Vol. 176, art. 112916, 2021. es_ES
dc.subject biosensor es_ES
dc.subject diffraction es_ES
dc.subject optical microfiber es_ES
dc.subject immunoassay es_ES
dc.subject non-specific binding es_ES
dc.subject label free es_ES
dc.title BIO Bragg gratings on microfibers for label-free biosensing es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.type info:eu-repo/semantics/other es_ES
dc.subject.unesco UNESCO::FÍSICA es_ES
dc.identifier.doi https://doi.org/10.1016/j.bios.2020.112916 es_ES
dc.accrualMethod - es_ES
dc.embargo.terms 2 year es_ES

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