Investigation of PTEN promoter methylation in ameloblastoma
NAGIOS: RODERIC FUNCIONANDO

Investigation of PTEN promoter methylation in ameloblastoma

DSpace Repository

Investigation of PTEN promoter methylation in ameloblastoma

Show simple item record

dc.contributor.author Lapthanasupkul, Puangwan es
dc.contributor.author Klongnoi, Boworn es
dc.contributor.author Mutirangura, Apiwat es
dc.contributor.author Kitkumthorn, Nakarin es
dc.date.accessioned 2021-01-15T09:31:03Z
dc.date.available 2021-01-15T09:31:03Z
dc.date.issued 2020 es
dc.identifier.uri https://hdl.handle.net/10550/77152
dc.description.abstract Phosphatase and tensin homolog (PTEN) acts as a tumor suppressor gene. Inactivation of PTEN has been reported in various types of cancers. PTEN promoter methylation possibly underlies PTEN inactivation, which results in tumorigenesis. The aim of this study was to investigate whether PTEN promoter methylation contributes to PTEN inactivation in ameloblastoma and its associated protein expression. In total, 20 fresh-frozen ameloblastoma samples were evaluated for PTEN promoter methylation using methylation-specific polymerase chain reaction (MS-PCR). A subset of 10 paraffin-embedded ameloblastoma samples was examined for PTEN expression through immunohistochemistry. Four primary cultured ameloblastoma cells were investigated for PTEN promoter methylation and PTEN transcriptional expression via reverse transcription PCR. PTEN promoter methylation was detected in 65% (13/20) of the ameloblastoma samples. Of 10 ameloblastoma samples, 4 exhibited reduced PTEN expression. Of 5 samples with methylated PTEN, 3 (60%) were associated with loss of PTEN expression. However, PTEN expression was detected in 4 (80%) of 5 samples with unmethylated PTEN. In addition, 3 (75%) of 4 primary ameloblastoma cell cultures exhibited an inverse correlation between PTEN promoter methylation and PTEN transcription level. PTEN promoter methylation is found in a number of ameloblastomas but not significantly correlated with loss of PTEN expression. Genetic or epigenetic mechanisms other than PTEN promoter methylation may contribute to PTEN inactivation in ameloblastoma tumor cells. es
dc.source Lapthanasupkul, Puangwan ; Klongnoi, Boworn ; Mutirangura, Apiwat ; Kitkumthorn, Nakarin. Investigation of PTEN promoter methylation in ameloblastoma. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 25 4 2020: 8- es
dc.title Investigation of PTEN promoter methylation in ameloblastoma es
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.23498 es

View       (1.652Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics