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Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy

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Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy

dc.contributor.author	Scrofani, G.
dc.contributor.author	Saavedra Tortosa, Genaro
dc.contributor.author	Martínez Corral, Manuel
dc.contributor.author	Sánchez Ortiga, Emilio
dc.date.accessioned	2020-12-11T16:42:00Z
dc.date.available	2020-12-11T16:42:00Z
dc.date.issued	2020
dc.identifier.uri	https://hdl.handle.net/10550/76642
dc.description.abstract	We report a protocol that takes advantage of the Fourier lightfield microscopy concept for providing 3D darkfield images of volumetric samples in a single-shot. This microscope takes advantage of the Fourier lightfield configuration, in which a lens array is placed at the Fourier plane of the microscope objective, providing a direct multiplexing of the spatio-angular information of the sample. Using the proper illumination beam, the system collects the light scattered by the sample while the background light is blocked out. This produces a set of orthographic perspective images with shifted spatial-frequency components that can be recombined to produce a 3D darkfield image. Applying the adequate reconstruction algorithm high-contrast darkfield optical sections are calculated in real time. The presented method is applied for fast volumetric reconstructions of unstained 3D samples.
dc.language.iso	eng
dc.relation.ispartof	Optics Express, 2020, vol. 28, p. 30513-30519
dc.source	Scrofani, Gabriele Saavedra Tortosa, Genaro Martínez Corral, Manuel Sánchez Ortiga, Emilio 2020 Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy Optics Express 28 30513 30519
dc.subject	Òptica
dc.subject	Microscòpia
dc.title	Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy
dc.type	journal article	es_ES
dc.date.updated	2020-12-11T16:42:00Z
dc.identifier.doi	https://doi.org/10.1364/OE.404961
dc.identifier.idgrec	141917
dc.rights.accessRights	open access	es_ES