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Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy

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	Scrofani, G.; Saavedra Tortosa, Genaro; Martínez Corral, Manuel Perfil; Sánchez Ortiga, Emilio
	This document is a artículoDate2020
	We report a protocol that takes advantage of the Fourier lightfield microscopy concept for providing 3D darkfield images of volumetric samples in a single-shot. This microscope takes advantage of the Fourier lightfield configuration, in which a lens array is placed at the Fourier plane of the microscope objective, providing a direct multiplexing of the spatio-angular information of the sample. Using the proper illumination beam, the system collects the light scattered by the sample while the background light is blocked out. This produces a set of orthographic perspective images with shifted spatial-frequency components that can be recombined to produce a 3D darkfield image. Applying the adequate reconstruction algorithm high-contrast darkfield optical sections are calculated in real time. The presented method is applied for fast volumetric reconstructions of unstained 3D samples.
	Scrofani, Gabriele Saavedra Tortosa, Genaro Martínez Corral, Manuel Sánchez Ortiga, Emilio 2020 Three-dimensional real-time darkfield imaging through Fourier lightfield microscopy Optics Express 28 30513 30519
	https://doi.org/10.1364/OE.404961