Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1α Activation.
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Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1α Activation.

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Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1α Activation.

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dc.contributor.author Mas Bargues, Cristina
dc.contributor.author Sanz Ros, Jorge
dc.contributor.author Román Domínguez, Aurora
dc.contributor.author Gimeno Mallench, Lucia
dc.contributor.author Inglés de la Torre, Marta
dc.contributor.author Viña Ribes, José
dc.contributor.author Borrás, Consuelo
dc.date.accessioned 2020-11-24T09:57:05Z
dc.date.available 2020-11-24T09:57:05Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/10550/76453
dc.description.abstract Aging is accompanied by the accumulation of senescent cells that alter intercellular communication, thereby impairing tissue homeostasis and reducing organ regenerative potential. Recently, the administration of mesenchymal stem cells (MSC)-derived extracellular vesicles has proven to be more effective and less challenging than current stem cell-based therapies. Extracellular vesicles (EVs) contain a cell-specific cargo of proteins, lipids and nucleic acids that are released and taken up by probably all cell types, thereby inducing functional changes via the horizontal transfer of their cargo. Here, we describe the beneficial properties of extracellular vesicles derived from non-senescent MSC, cultured in a low physiological oxygen tension (3%) microenvironment into prematurely senescent MSC, cultured in a hyperoxic ambient (usual oxygen culture conditions, i.e., 21%). We observed that senescent MCS, treated with EVs from non-senescent MCS, showed reduced SA-β-galactosidase activity levels and pluripotency factor (OCT4, SOX2, KLF4 and cMYC, or OSKM) overexpression and increased glycolysis, as well as reduced oxidative phosphorylation (OXPHOS). Moreover, these EVs' cargo induced the upregulation of miR-302b and HIF-1α levels in the target cells. We propose that miR-302b triggered HIF-1α upregulation, which in turn activated different pathways to delay premature senescence, improve stemness and switch energetic metabolism towards glycolysis. Taken together, we suggest that EVs could be a powerful tool to restore altered intercellular communication and improve stem cell function and stemness, thus delaying stem cell exhaustion in aging.
dc.language.iso eng
dc.relation.ispartof Biomolecules, 2020, vol. Jun 25, num. 10(6), p. 957
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Mas Bargues, Cristina Sanz Ros, Jorge Román Domínguez, Aurora Gimeno Mallench, Lucia Inglés de la Torre, Marta Viña Ribes, José Borrás, Consuelo 2020 Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1α Activation. Biomolecules Jun 25 10(6) 957
dc.subject Fisiologia humana
dc.title Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1α Activation.
dc.type info:eu-repo/semantics/article
dc.date.updated 2020-11-24T09:57:06Z
dc.identifier.doi https://doi.org/10.3390/biom10060957
dc.identifier.idgrec 141368

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