RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib : a randomized clinical trialy
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RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib : a randomized clinical trialy

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RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib : a randomized clinical trialy

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dc.contributor.author Medeiros Albuquerque, Assis Filipe es
dc.contributor.author Sá Roriz Fonteles, Cristiane es
dc.contributor.author do Nascimento Costa, José Jackson es
dc.contributor.author Silva, José Roberto Viana es
dc.contributor.author Silva, Paulo-Goberlânio-de Barros es
dc.contributor.author Soares, Eduardo Costa Studart es
dc.contributor.author Chaves, Filipe Nobre es
dc.contributor.author Alves Pereira, Karuza Maria es
dc.contributor.author Rodrigues-Ribeiro, Thyciana es
dc.contributor.author Gurgel Costa, Fábio Wildson es
dc.date.accessioned 2020-10-22T11:50:51Z
dc.date.available 2020-10-22T11:50:51Z
dc.date.issued 2020 es
dc.identifier.uri https://hdl.handle.net/10550/76031
dc.description.abstract Background: This study aimed to evaluate the gene expression of cyclooxygenases (COXs) in an oral model of preemptive analgesia. Material and Methods: Gingival tissue was collected during extraction of lower third molars from a randomized, triple-blind, split-mouth and placebo-controlled study. The eligible patients were randomly sorted to receive a single dose either of ibuprofen 400mg, or etoricoxib 120 mg or a placebo, one hour prior to surgery. The temporal course of RNAm was evaluated for COX-1 and -2 by means of a quantitative polymerase chain reaction in real time (RT-qPCR) at time zero and 30 minutes after the surgical procedure began, and it was correlated with clinical parameters (pain and maximum mouth opening). Results: There was a significant increase in COX-1 expression between T0 and T30 in ibuprofen (p=0.004) and eto-ricoxib (p=0.010) groups. As regards COX-2, there were increases from T0 to T30 in all groups (placebo, p=0.012; ibuprofen, p<0.001; etoricoxib, p<0.001). All groups showed a significant decrease in COX-2:COX-1 ratio from T0 to T30 (placebo, p=0.013; ibuprofen, p<0.001; etoricoxib, p=0.047). Experimental groups showed a significant correlation between COX-1 and COX-2 levels and clinical pain parameters. Conclusions: The present preemptive analgesia study concludes that COX-2 RNAm induction was directly linked to third molar-related tissue inflammation and that the relation between COX-1 and COX-2 levels were inversely proportional to the preemptively administered nonsteroidal anti-inflammatory drugs COX-2 selectivity. es
dc.source Medeiros Albuquerque, Assis Filipe ; Sá Roriz Fonteles, Cristiane ; do Nascimento Costa, José Jackson ; Silva, José Roberto Viana ; Silva, Paulo-Goberlânio-de Barros ; Soares, Eduardo Costa Studart ; Chaves, Filipe Nobre ; Alves Pereira, Karuza Maria ; Rodrigues-Ribeiro, Thyciana ; Gurgel Costa, Fábio Wildson. RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib : a randomized clinical trialy. En: Journal of Clinical and Experimental Dentistry, 12 4 2020: 371-380 es
dc.title RT-qPCR study of COX-1 and -2 genes in oral surgical model comparing single-dose preemptive ibuprofen and etoricoxib : a randomized clinical trialy es
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/jced.56447 es

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