Translational repression and novel functions of Cth2 in the Saccharomyces cerevisiae response to iron deficiency
NAGIOS: RODERIC FUNCIONANDO

Translational repression and novel functions of Cth2 in the Saccharomyces cerevisiae response to iron deficiency

DSpace Repository

Translational repression and novel functions of Cth2 in the Saccharomyces cerevisiae response to iron deficiency

Show simple item record

dc.contributor.advisor Puig Todolí, Sergi
dc.contributor.advisor Martínez Pastor, María Teresa
dc.contributor.author Ramos Alonso, Lucía
dc.contributor.other Departament de Bioquímica i Biologia Molecular es_ES
dc.date.accessioned 2020-09-25T07:53:52Z
dc.date.available 2020-09-26T04:45:05Z
dc.date.issued 2020 es_ES
dc.date.submitted 02-10-2020 es_ES
dc.identifier.uri https://hdl.handle.net/10550/75653
dc.description.abstract El hierro (Fe) es un micronutriente y cofactor esencial para todos los organismos eucariotas. Las proteínas que incorporan hierro en su estructura lo hacen en forma de grupos hemo, centros Fe/S o centros de hierro y oxígeno, entre otros. Estos cofactores están implicados en numerosos procesos celulares como la respiración, la replicación y reparación del DNA, la biogénesis de ribosomas y la traducción de proteínas, la biosíntesis de ácidos nucleicos y lípidos, la fotosíntesis y el transporte de oxígeno. Pese a ser un metal abundante en la corteza terrestre, su forma oxidada Fe+3 es la más frecuente en un entorno oxidante y resulta insoluble a pH fisiológico. Esto hace de la deficiencia de hierro o anemia ferropénica el desorden nutricional más extendido del planeta. Alteraciones en la homeostasis del hierro también provocan hemocromatosis, o algunas enfermedades neurodegenerativas graves como la ataxia de Friedrich, y contribuyen al cáncer. Además, la deficiencia de hierro también provoca clorosis en los cultivos agrícolas, afectando tanto su producción como su valor nutricional. A pesar de ser un nutriente esencial, la presencia de hierro en exceso es tóxica para las células ya que participa en reacciones Fenton. Estas reacciones generan radicales hidroxilo (·OH) a partir del peróxido de hidrógeno (H2O2), los cuales pueden oxidar proteínas, DNA o lípidos si la maquinaria de detoxificación de especies reactivas del oxígeno (ROS) se ve sobrepasada. es_ES
dc.format.extent 252 p. es_ES
dc.language.iso en es_ES
dc.subject iron es_ES
dc.subject deficiency es_ES
dc.subject cth2 es_ES
dc.subject rnr3 es_ES
dc.subject yeast es_ES
dc.subject saccharomyces es_ES
dc.subject cerevisiae es_ES
dc.subject respiration es_ES
dc.subject mitochondria es_ES
dc.subject translation es_ES
dc.subject polysomes es_ES
dc.title Translational repression and novel functions of Cth2 in the Saccharomyces cerevisiae response to iron deficiency es_ES
dc.type info:eu-repo/semantics/doctoralThesis es_ES
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Biología molecular es_ES
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA::Microbiología es_ES
dc.description.abstractenglish Iron (Fe) is an essential micronutrient for all eukaryotes because of its redox properties. It participates as a cofactor in a wide range of biological processes, including protein translation, respiration (Krebs cycle and electron transport chain (ETC)) and DNA replication. The model organism Saccharomyces cerevisiae responds to iron deficiency (-Fe) by activating the Fe acquisition and recycling systems, and by remodeling cellular metabolism to promote Fe utilization in specific processes. The tandem zinc finger (TZF)-containing protein Cth2 plays an important role in prioritizing Fe by promoting the degradation of multiple mRNAs containing A/U-rich elements (AREs), including the CTH2 mRNA itself that is autoregulated. In this thesis, we identified and characterized new mechanisms involved in the global translational repression and novel functions of Cth2 in response to iron deficiency. Our results with polysome fractionation experiments demonstrate that the eIF2α/Gcn2 pathway is involved in the general repression of translational initiation during iron deficiency. The Gcn2 kinase specifically phosphorylates serine 51 of eIF2α in a Gcn1-dependent manner, causing a slight induction of GCN4 translation. The Gcn2 activation by uncharged tRNAs and TORC1 inactivation under iron deficiency is discussed. Besides, we show a role played by Cth2 in translational inhibition of several ARE-mRNAs in -Fe. Both the Cth2 TZF-domain as well as the AREs within SDH4 and CTH2 mRNAs are essential for translational repression, and we suggest a Cth2 general role on inhibition of translation of several ARE-mRNAs. Besides, while the amino-terminal domain (NTD) of Cth2 is important for both mRNA turnover and translational inhibition, its carboxy-terminal domain (CTD) is only involved in translational repression. Importantly, Cth2 carboxy-terminal domain is physiologically relevant in iron deficiency, and its mutation increases the protein levels of several Cth2 targets. Two novel Cth2 functions in -Fe include the regulation of mitochondrial respiration and the RNR3 catalytic subunit of the Fe-dependent ribonucleotide reductase (RNR) responsible of dNTP synthesis. The overexpression of CTH2 under Fe sufficiency decreases oxygen consumption as well as several Fe-enzymatic activities (Leu1, aconitase, complex II and III of the electron transport chain) while the complex IV activity is unaffected. Under iron deficient conditions, oxygen consumption decreases regardless Cth2, despite the decrease in complex II and III that is more pronounced when CTH2 is expressed. However the complex IV enzymatic activity is maintained by Cth2, probably by increasing Cox1 protein levels. Finally, RNR3 has been described to be highly expressed under genotoxic or replication stress by the Mec1–Rad53–Dun1 checkpoint pathway. However, RNR3 function is not clear as it is not the major isoform of the catalytic subunit of the enzyme ribonucleotide reductase. Our results suggest a higher RNR3 expression only under long-term iron deficiency comparable to that observed under those stresses. Besides, we demonstrate the participation of Cth2 (through its tandem zinc fingers) and Dun1 in the transcriptional induction of RNR3 under long-term iron deficient conditions. Cth2 partially promotes the RNR3 transcriptional derepression through Crt1 and Rox1 regulation under long-term iron deficiency, both transcriptional repressors of the RNR3 promoter. Importantly, unlike other stresses, RNR3 is physiologically relevant in iron deficiency es_ES
dc.embargo.terms 0 days es_ES

View       (5.437Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics