Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing
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Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing

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Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing

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dc.contributor.author Palero Pastor, Ferran
dc.contributor.author García-Merchan, VH.
dc.contributor.author Abelló, P.
dc.contributor.author Macpherson, E.
dc.contributor.author Pascual, M.
dc.date.accessioned 2020-07-20T04:39:02Z
dc.date.available 2020-07-20T04:39:02Z
dc.date.issued 2012 es_ES
dc.identifier.uri https://hdl.handle.net/10550/75258
dc.description.abstract The portunid crab Liocarcinus depurator is the dominant decapod crustacean present on mud bottoms of the continental shelf of the Mediterranean Sea. In order to better describe its genetic structure, a total of 11 microsatellites were isolated and tested in 28 L. depurator individuals from the Mediterranean locality of Tarragona. Initially, microsatellite isolation was carried out using the FIASCO methodology, but only 2 useful primer pairs could be designed. Given the low yield obtained, an alternative method based on 454 next-generation sequencing was assayed, which provided excellent results and from which another 9 primer pairs gave positive genotyping. All loci tested were polymorphic, and 2 to 24 (mean 10.54) alleles per locus were identified. The observed and expected heterozygosities per locus ranged from 0.214 to 0.926 and from 0.232 to 0.962, respectively. One locus (Ldep10) showed the presence of null alleles according to the Brookfield estimator implemented in MICROCHECKER. Nevertheless, none of the markers deviated significantly from Hardy–Weinberg equilibrium or showed evidence of linkage disequilibrium. This set of 11 markers is being used to study the population structure and genetic diversity of Liocarcinus depurator populations from both Mediterranean and adjacent Atlantic waters. es_ES
dc.language.iso es es_ES
dc.source Palero F; Garcia-Merchan VH; Abelló P; Macpherson E; Pascual M (2012). Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing. In: Molecular Ecology Resources Primer Development Consortium et al. (2012). Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2012-31 May 2012. Molecular Ecology Resources, 12 (5), 972 - 974. es_ES
dc.subject portunidae es_ES
dc.subject decapod crustaceans es_ES
dc.subject pyrosequencing es_ES
dc.subject SSRs es_ES
dc.title Isolation and characterization of microsatellite markers in the portunid crab Liocarcinus depurator using FIASCO and 454 next-generation sequencing es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.subject.unesco UNESCO::CIENCIAS DE LA VIDA es_ES
dc.identifier.doi https://doi.org/10.1111/j.1755-0998.2012.03173.x es_ES
dc.description.abstractenglish The portunid crab Liocarcinus depurator is the dominant decapod crustacean present on mud bottoms of the continental shelf of the Mediterranean Sea. In order to better describe its genetic structure, a total of 11 microsatellites were isolated and tested in 28 L. depurator individuals from the Mediterranean locality of Tarragona. Initially, microsatellite isolation was carried out using the FIASCO methodology, but only 2 useful primer pairs could be designed. Given the low yield obtained, an alternative method based on 454 next-generation sequencing was assayed, which provided excellent results and from which another 9 primer pairs gave positive genotyping. All loci tested were polymorphic, and 2 to 24 (mean 10.54) alleles per locus were identified. The observed and expected heterozygosities per locus ranged from 0.214 to 0.926 and from 0.232 to 0.962, respectively. One locus (Ldep10) showed the presence of null alleles according to the Brookfield estimator implemented in MICROCHECKER. Nevertheless, none of the markers deviated significantly from Hardy–Weinberg equilibrium or showed evidence of linkage disequilibrium. This set of 11 markers is being used to study the population structure and genetic diversity of Liocarcinus depurator populations from both Mediterranean and adjacent Atlantic waters. es_ES
dc.identifier.idgrec 078297 es_ES

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