Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture
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Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture

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Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture

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dc.contributor.author Jorda, Adrian
dc.contributor.author Aldasoro Celaya, Martín
dc.contributor.author Aldasoro, Constanza
dc.contributor.author Guerra Ojeda, Sol
dc.contributor.author Iradi Casal, Antonio
dc.contributor.author Vila Salinas, José María
dc.contributor.author Campos Campos, Juan
dc.contributor.author Vallés Martí, Lilián Soraya
dc.date.accessioned 2020-06-22T07:26:53Z
dc.date.available 2020-06-22T07:26:53Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/10550/75151
dc.description.abstract Aspirin has been used as anti-inflammatory and anti-aggregate for decades but the precise mechanism(s) of action after the presence of the toxic peptide Aβ1-42 in cultured astrocytes remains poorly resolved. Here we use low-doses of aspirin (10-7 M) in astrocytes in primary culture in presence or absence of Aβ1-42 toxic peptide. We noted an increase of cell viability and proliferation with or without Aβ1-42 peptide presence in aspirin treated cells. In addition, a decrease in apoptosis, determined by Caspase 3 activity and the expression of Cyt c and Smac/Diablo, were detected. Also, aspirin diminished necrosis process (LDH levels), pro-inflammatory mediators (IL-β and TNF-α) and NF-ᴋB protein expression, increasing anti-inflammatory PPAR-γ protein expression, preventing Aβ1-42 toxic effects. Aspirin inhibited COX-2 and iNOS without changes in COX-1 expression, increasing anti-oxidant protein (Cu/Zn-SOD and Mn-SOD) expression in presence or absence of Aβ1-42. Taken together, our results show that aspirin, at low doses increases cell viability by decreasing inflammation and oxidative stress, preventing the deleterious effects of the Aβ1-42 peptide on astrocytes in primary culture. The use of low doses of aspirin may be more suitable for Alzheimer's disease.
dc.language.iso eng
dc.relation.ispartof International Journal Of Medical Sciences, 2020, vol. 17, p. 834-843
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Jorda, Adrian Aldasoro Celaya, Martín Aldasoro, Constanza Guerra Ojeda, Sol Iradi Casal, Antonio Vila Salinas, José María Campos Campos, Juan Vallés Martí, Lilián Soraya 2020 Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture International Journal Of Medical Sciences 17 834 843
dc.title Action of low doses of Aspirin in Inflammation and Oxidative Stress induced by aβ1-42 on Astrocytes in primary culture
dc.type info:eu-repo/semantics/article
dc.date.updated 2020-06-22T07:26:53Z
dc.identifier.doi https://doi.org/10.7150/ijms.40959
dc.identifier.idgrec 139700

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