Sero-epidemiological study of the rotavirus VP8* protein from different P genotypes in Valencia, Spain
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Sero-epidemiological study of the rotavirus VP8* protein from different P genotypes in Valencia, Spain

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Sero-epidemiological study of the rotavirus VP8* protein from different P genotypes in Valencia, Spain

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dc.contributor.author Vila Vicent, Susana
dc.contributor.author Gozalbo Rovira, Roberto Vicente
dc.contributor.author Rubio-Del-Campo, Antonio
dc.contributor.author Santiso Bellón, Cristina
dc.contributor.author Navarro Lleó, Noemí
dc.contributor.author Muñoz Collado, Carlos
dc.contributor.author Buesa Gómez, Javier
dc.contributor.author Rodríguez Díaz, Jesús
dc.date.accessioned 2020-06-09T10:21:34Z
dc.date.available 2020-06-09T10:21:34Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/10550/74956
dc.description.abstract The aims of the present work were to determine the prevalence and titer of serum antibodies against several rotavirus VP8* proteins from different P genotypes in children and adults in Valencia, Spain; and to determine the role of the secretor status (FUT2G428A polymorphism) in the antibody response. The VP8* protein from the P[4], P[6], P[8], P[9], P[11], P[14] and P[25] genotypes were produced in E. coli. These proteins were tested with 88 serum samples from children (n = 41, 3.5 years old in average) and from adults (n = 47, 58 years old in average) by ELISA. A subset of 55 samples were genotyped for the FUT2G428A polymorphism and the antibody titers compared. The same subset of samples was also analysed by ELISA using whole rotavirus Wa particles (G1P[8]) as antigen. Ninety-three per cent of the samples were positive for at least one of the VP8* antigens. Differences in the IgG seroprevalence were found between children and adults for the P[4], P[8] and P[11] genotypes. Similarly, significant differences were found between adults and children in their antibody titers against the P[4], P[8], and P[11] VP8* genotypes, having the children higher antibody titers than adults. Interestingly, positive samples against rare genotypes such as P[11] (only in children), P[14] and P[25] were found. While no statistical differences in the antibody titers between secretors and non-secretors were found for any of the tested P genotypes studied, a higher statistic significant prevalence for the P[25] genotype was found in secretors compared to non-secretors. Significant differences in the antibody titers between secretors and non-secretors were found when the whole viral particles from the Wa rotavirus strain (G1P[8]) were used as the antigen.
dc.language.iso eng
dc.relation.ispartof Scientific Reports, 2020, vol. 10, num. 7753
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Vila Vicent, Susana Gozalbo Rovira, Roberto Vicente Rubio-Del-Campo, Antonio Santiso Bellón, Cristina Navarro Lleó, Noemí Muñoz Collado, Carlos Buesa Gómez, Javier Rodríguez Díaz, Jesús 2020 Sero-epidemiological study of the rotavirus VP8* protein from different P genotypes in Valencia, Spain Scientific Reports 10 7753
dc.subject Virus RNA
dc.subject Epidemiologia
dc.title Sero-epidemiological study of the rotavirus VP8* protein from different P genotypes in Valencia, Spain
dc.type info:eu-repo/semantics/article
dc.date.updated 2020-06-09T10:21:35Z
dc.identifier.doi https://doi.org/10.1038/s41598-020-64767-x
dc.identifier.idgrec 139437

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