Type 1 diabetic mellitus patientswith increased atherosclerosisriskdisplay decreased CDKN2A/2B/2BAS gene expression in leukocytes
NAGIOS: RODERIC FUNCIONANDO

Type 1 diabetic mellitus patientswith increased atherosclerosisriskdisplay decreased CDKN2A/2B/2BAS gene expression in leukocytes

DSpace Repository

Type 1 diabetic mellitus patientswith increased atherosclerosisriskdisplay decreased CDKN2A/2B/2BAS gene expression in leukocytes

Show simple item record

dc.contributor.author Martínez Hervás, Sergio
dc.contributor.author Sánchez-García, Verónica
dc.contributor.author Herrero-Cervera, Andrea
dc.contributor.author Vinué Visús, María Ángela
dc.contributor.author Real Collado, José Tomás
dc.contributor.author Ascaso Gimilio, Juan Francisco
dc.contributor.author Burks, Deborah Jane
dc.contributor.author González Navarro, Herminia
dc.date.accessioned 2020-04-30T07:55:15Z
dc.date.available 2020-04-30T07:55:15Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10550/74139
dc.description.abstract Background: Type 1 diabetes mellitus (T1DM) patients display increased risk of cardiovascular disease (CVD) and are characterized by a diminished regulatory T (Treg) cell content or function. Previous studies have shown an association between decreased CDKN2A/2B/2BAS gene expression and enhanced CVD. In the present study the potential relationship between CDKN2A/2B/2BAS gene expression, immune cell dysfunction and increased cardiovascular risk in T1DM patients was explored. Methods: A cross-sectional study was performed in 90 subjects divided into controls and T1DM patients. Circulating leukocyte subpopulations analysis by flow cytometry, expression studies on peripheral blood mononuclear cell by qPCR and western blot and correlation studies were performed in both groups of subjects. Results: Analysis indicated that, consistent with the described T cell dysfunction, T1DM subjects showed decreased circulating CD4+CD25+CD127- Treg cells. In addition, T1DM subjects had lower mRNA levels of the transcription factors FOXP3 and RORC and lower levels of IL2 and IL6 which are involved in Treg and Th17 cell differentiation, respectively. T1DM patients also exhibited decreased mRNA levels of CDKN2A (variant 1 p16Ink4a), CDKN2A (p14Arf, variant 4), CDKN2B (p15Ink4b) and CDKN2BAS compared with controls. Notably, T1DM patients had augmented pro-atherogenic CD14++CD16+-monocytes, which predict cardiovascular acute events and enhanced common carotid intima-media thickness (CC-IMT). Conclusions: Decreased expression of CDKN2A/2B/2BAS in leukocytes associates with increased CC-IMT atherosclerosis surrogate marker and proatherogenic CD14++CD16+ monocytes in T1DM patients. These results suggest a potential role of CDKN2A/2B/2BAS genes in CVD risk in T1DM.
dc.language.iso eng
dc.relation.ispartof Journal Of Translational Medicine, 2019, vol. 17, num. 1, p. 222
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Martínez Hervás, Sergio Sánchez-García, Verónica Herrero-Cervera, Andrea Vinué Visús, María Ángela Real Collado, José Tomás Ascaso Gimilio, Juan Francisco Burks, Deborah Jane González Navarro, Herminia 2019 Type 1 diabetic mellitus patientswith increased atherosclerosisriskdisplay decreased CDKN2A/2B/2BAS gene expression in leukocytes Journal Of Translational Medicine 17 1 222
dc.subject Diabetis
dc.title Type 1 diabetic mellitus patientswith increased atherosclerosisriskdisplay decreased CDKN2A/2B/2BAS gene expression in leukocytes
dc.type info:eu-repo/semantics/article
dc.date.updated 2020-04-30T07:55:15Z
dc.identifier.doi https://doi.org/10.1186/s12967-019-1977-1
dc.identifier.idgrec 138522

View       (1.131Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics