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Optical sectioning microscopy through single-shot Lightfield protocol

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	Sánchez Ortiga, Emilio; Scrofani, G.; Saavedra Tortosa, Genaro; Martínez Corral, Manuel Perfil
	This document is a artículoDate2020
	Optical sectioning microscopy is usually performed by means of a scanning, multi-shot procedure in combination with non-uniform illumination. In this paper, we change the paradigm and report a method that is based in the light field concept, and that provides optical sectioning for 3D microscopy images after a single-shot capture. To do this we fi rst capture multiple orthographic perspectives of the sample by means of Fourier-domain integral microscopy (FiMic). The second stage of our protocol is the application of a novel refocusing algorithm that is able to produce optical sectioning in real time, and with no resolution worsening, in the case of sparse f luorescent samples.We provide the theoretical derivation of the algorithm, and demonstrate its utility by applying it to simulations and to experimental data.
	Sánchez Ortiga, Emilio Scrofani, G. Saavedra Tortosa, Genaro Martínez Corral, Manuel 2020 Optical sectioning microscopy through single-shot Lightfield protocol Ieee Access 8 14944 14952
	https://doi.org/10.1109/ACCESS.2020.2966323