N-Linked Glycosylation of the p24 Family Protein p24δ5 Modulates Retrograde Golgi-to-ER Transport of K/HDEL Ligands in Arabidopsis
NAGIOS: RODERIC FUNCIONANDO

N-Linked Glycosylation of the p24 Family Protein p24δ5 Modulates Retrograde Golgi-to-ER Transport of K/HDEL Ligands in Arabidopsis

DSpace Repository

N-Linked Glycosylation of the p24 Family Protein p24δ5 Modulates Retrograde Golgi-to-ER Transport of K/HDEL Ligands in Arabidopsis

Show simple item record

dc.contributor.author Pastor Cantizano, Noelia
dc.contributor.author García Murria, María Jesús
dc.contributor.author Bernat Silvestre, César
dc.contributor.author Marcote Zaragoza, María Jesús
dc.contributor.author Mingarro Muñoz, Ismael
dc.contributor.author Aniento Company, Fernando
dc.date.accessioned 2019-11-12T11:35:01Z
dc.date.available 2019-11-12T11:35:01Z
dc.date.issued 2017
dc.identifier.uri https://hdl.handle.net/10550/72176
dc.description.abstract The K/HDEL receptor ERD2 mediates the transport of soluble endoplasmic reticulum (ER)-resident proteins containing a C-terminal K/HDEL signal from the Golgi apparatus back to the ER via COPI (COat Protein I)-coated vesicles. Sorting of ERD2 within COPI vesicles is facilitated by p24 proteins. In Arabidopsis, p24δ5 has been shown to interact directly with ERD2 via its luminal GOLD (GOLgi Dynamics) domain and with COPI proteins via its cytoplasmic C-terminal tail at the acidic pH of the Golgi apparatus. Several members of the p24 family in mammals and yeast have been shown to be glycosylated, but whether Arabidopsis p24 proteins are glycosylated and the role of the sugar moiety in p24 function remain unclear. Here, we show that Arabidopsis p24δ5 protein is N-glycosylated in its GOLD domain. Furthermore, we demonstrate that this post-translational modification is important for its coupled transport with p24β2 at the ER-Golgi interface, for its interaction with the K/HDEL receptor ERD2, and for retrograde transport of ERD2 and K/HDEL ligands from the Golgi apparatus back to the ER.
dc.language.iso eng
dc.relation.ispartof Molecular Plant, 2017, vol. 10, num. 8, p. 1095-1106
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Pastor Cantizano, Noelia García Murria, María Jesús Bernat Silvestre, César Marcote Zaragoza, María Jesús Mingarro Muñoz, Ismael Aniento Company, Fernando 2017 N-Linked Glycosylation of the p24 Family Protein p24δ5 Modulates Retrograde Golgi-to-ER Transport of K/HDEL Ligands in Arabidopsis Molecular Plant 10 8 1095 1106
dc.subject Biotecnologia
dc.subject Proteïnes
dc.title N-Linked Glycosylation of the p24 Family Protein p24δ5 Modulates Retrograde Golgi-to-ER Transport of K/HDEL Ligands in Arabidopsis
dc.type info:eu-repo/semantics/article
dc.date.updated 2019-11-12T11:35:01Z
dc.identifier.doi https://doi.org/10.1016/j.molp.2017.07.007
dc.identifier.idgrec 123477

View       (5.653Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics