Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy
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Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy

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Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy

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dc.contributor.author Sola Pikabea, Jorge
dc.contributor.author García Rius, Arcadi
dc.contributor.author Saavedra Tortosa, Genaro
dc.contributor.author García Sucerquia, Jorge Iván
dc.contributor.author Martínez Corral, Manuel
dc.contributor.author Sánchez Ortiga, Emilio
dc.date.accessioned 2019-09-17T13:35:23Z
dc.date.available 2019-09-17T13:35:23Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10550/71543
dc.description.abstract A method of determining unknown phase-shifts between elementary images in two-dimensional Structured Illumination Microscopy (2D-SIM) is presented. The proposed method is based on the comparison of the peak intensity of spectral components. These components correspond to the inherent structured illumination spectral content and the residual compo- nent that appears from wrongly estimated phase-shifts. The estimation of the phase-shifts is carried out by finding the absolute maximum of a function defined as the normalized peak intensity difference in the Fourier domain. This task is performed by an optimization method providing a fast estimation of the phase-shift. The algorithm stability and robustness are tested for various levels of noise and contrasts of the structured illumination pattern. Furthermore, the proposed approach reduces the number of computations compared to other existing techniques. The method is supported by the theoretical calculations and validated by means of simulated and experimental results.
dc.language.iso eng
dc.relation.ispartof Plos One, 2019, vol. 14, num. 8, p. e0221254
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Sola Pikabea, Jorge García Rius, Arcadi Saavedra Tortosa, Genaro García Sucerquia, Jorge Iván Martínez Corral, Manuel Sánchez Ortiga, Emilio 2019 Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy Plos One 14 8 e0221254
dc.subject Microscòpia
dc.subject Fourier, Anàlisi de
dc.subject Imatges Processament Tècniques digitals
dc.title Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy
dc.type info:eu-repo/semantics/article
dc.date.updated 2019-09-17T13:35:23Z
dc.identifier.doi https://doi.org/10.1371/journal.pone.0221254
dc.identifier.idgrec 134175

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