Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing
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Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing

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Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing

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dc.contributor.author López-Galiano, María José
dc.contributor.author Sentandreu, Vicente
dc.contributor.author Martínez Ramírez, Amparo
dc.contributor.author Rausell Segarra, Carolina
dc.contributor.author Real García, María Dolores
dc.contributor.author Camañes, Gemma
dc.contributor.author Ruiz-Rivero, Omar
dc.contributor.author Crespo Salvador, Óscar
dc.contributor.author García Robles, Inmaculada
dc.date.accessioned 2019-07-22T09:14:00Z
dc.date.available 2019-07-22T09:14:00Z
dc.date.issued 2019
dc.identifier.uri https://hdl.handle.net/10550/70921
dc.description.abstract Tomato (Solanum lycopersicum) is one of the most important crops around the world and also a model plant to study response to stress. High-throughput sequencing was used to analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative PCR (RT-qPCR) was used to validate high-throughput expression analysis data, confirming the expression profiles of 10 out of 11 randomly selected miRNAs. Most of the differentially expressed miRNAs were stress-specific, except for sly-miR167c-3p upregulated in B. cinerea and P. syringae infection, sly-newmiR26-3p upregulated in drought and Hx treatment samples, and sly-newmiR33-3p, sly-newmiR6-3p and sly-newmiR8-3p differentially expressed both in biotic and abiotic stresses. From mature miRNAs sequences of the 41 stress-responsive miRNAs 279 targets were predicted. An inverse correlation between the expression profiles of 4 selected miRNAs (sly-miR171a, sly-miR172c, sly-newmiR22-3p and sly-miR167c-3p) and their target genes (Kinesin, PPR, GRAS40, ABC transporter, GDP and RLP1) was confirmed by RT-qPCR. Altogether, our analysis of miRNAs in different biotic and abiotic stress conditions highlight the interest to understand the functional role of miRNAs in tomato stress response as well as their putative targets which could help to elucidate plants molecular and physiological adaptation to stress.
dc.language.iso eng
dc.relation.ispartof Genes, 2019, vol. 10, num. 6, p. 475
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source López-Galiano, María José Sentandreu, Vicente Martínez Ramírez, Amparo Rausell Segarra, Carolina Real García, María Dolores Camañes, Gemma Ruiz-Rivero, Omar Crespo Salvador, Óscar García Robles, Inmaculada 2019 Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing Genes 10 6 475
dc.subject Tomàquets
dc.subject Estrès
dc.title Identification of stress associated microRNAs in Solanum lycopersicum by high-throughput Sequencing
dc.type info:eu-repo/semantics/article
dc.date.updated 2019-07-22T09:14:01Z
dc.identifier.doi https://doi.org/10.3390/genes10060475
dc.identifier.idgrec 133614

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