Large depth-of-field integral microscopy by use of a liquid Lens
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Large depth-of-field integral microscopy by use of a liquid Lens

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Large depth-of-field integral microscopy by use of a liquid Lens

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dc.contributor.author Llavador Ancheta, Ana Isabel
dc.contributor.author Scrofani, G.
dc.contributor.author Saavedra Tortosa, Genaro
dc.contributor.author Martínez Corral, Manuel
dc.date.accessioned 2018-10-11T13:47:54Z
dc.date.available 2018-10-11T13:47:54Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10550/67754
dc.description.abstract Integral microscopy is a 3D imaging technique that permits the recording of spatial and angular information of microscopic samples. From this information it is possible to calculate a collection of orthographic views with full parallax and to refocus computationally, at will, through the 3D specimen. An important drawback of integral microscopy, especially when dealing with thick samples, is the limited depth of field (DOF) of the perspective views. This imposes a significant limitation on the depth range of computationally refocused images. To overcome this problem, we propose here a new method that is based on the insertion, at the pupil plane of the microscope objective, of an electrically controlled liquid lens (LL) whose optical power can be changed by simply tuning the voltage. This new apparatus has the advantage of controlling the axial position of the objective focal plane while keeping constant the essential parameters of the integral microscope, that is, the magnification, the numerical aperture and the amount of parallax. Thus, given a 3D sample, the new microscope can provide a stack of integral images with complementary depth ranges. The fusion of the set of refocused images permits to enlarge the reconstruction range, obtaining images in focus over the whole region.
dc.language.iso eng
dc.relation.ispartof Sensors, 2018, vol. 18, num. 10, p. 3383
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Llavador Ancheta, Ana Isabel Scrofani, G. Saavedra Tortosa, Genaro Martínez Corral, Manuel 2018 Large depth-of-field integral microscopy by use of a liquid Lens Sensors 18 10 3383
dc.subject Imatges Processament
dc.subject Microscòpia
dc.title Large depth-of-field integral microscopy by use of a liquid Lens
dc.type info:eu-repo/semantics/article
dc.date.updated 2018-10-11T13:47:55Z
dc.identifier.doi https://doi.org/10.3390/s18103383
dc.identifier.idgrec 127899

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