The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock
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The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock

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The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock

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dc.contributor.author Garré García, Elena
dc.contributor.author Pelechano García, Vicente José
dc.contributor.author Sánchez Del Pino, Manuel
dc.contributor.author Alepuz Martínez, Paula
dc.contributor.author Sunnerhagen, P.
dc.date.accessioned 2018-10-03T15:05:24Z
dc.date.available 2018-10-03T15:05:24Z
dc.date.issued 2018
dc.identifier.uri http://hdl.handle.net/10550/67699
dc.description.abstract RNA-binding proteins (RBPs) establish the cellular fate of a transcript, but an understanding of these processes has been limited by a lack of identified specific interactions between RNA and protein molecules. Using MS2 RNA tagging, we have purified proteins associated with individual mRNA species induced by osmotic stress, STL1 and GPD1. We found members of the Lsm1-7/Pat1 RBP complex to preferentially bind these mRNAs, relative to the non-stress induced mRNAs, HYP2 and ASH1. To assess the functional importance, we mutated components of the Lsm1-7/Pat1 RBP complex and analyzed the impact on expression of osmostress gene products. We observed a defect in global translation inhibition under osmotic stress in pat1 and lsm1 mutants, which correlated with an abnormally high association of both non-stress and stress-induced mRNAs to translationally active polysomes. Additionally, for stress-induced proteins normally triggered only by moderate or high osmostress, in the mutants the protein levels rose high already at weak hyperosmosis. Analysis of ribosome passage on mRNAs through co-translational decay from the 5' end (5P-Seq) showed increased ribosome accumulation in lsm1 and pat1 mutants upstream of the start codon. This effect was particularly strong for mRNAs induced under osmostress. Thus, our results indicate that, in addition to its role in degradation, the Lsm1-7/Pat1 complex acts as a selective translational repressor, having stronger effect over the translation initiation of heavily expressed mRNAs. Binding of the Lsm1-7/Pat1p complex to osmostress-induced mRNAs mitigates their translation, suppressing it in conditions of weak or no stress, and avoiding a hyperresponse when triggered.
dc.language.iso eng
dc.relation.ispartof Plos Genetics, 2018, vol. 14, num. 7, p. e1007563
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Garré García, Elena Pelechano García, Vicente José Sánchez Del Pino, Manuel Alepuz Martínez, Paula Sunnerhagen, P. 2018 The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock Plos Genetics 14 7 e1007563
dc.subject RNA
dc.subject Ribosomes
dc.subject Expressió genètica
dc.title The Lsm1-7/Pat1 complex binds to stress-activated mRNAs and modulates the response to hyperosmotic shock
dc.type info:eu-repo/semantics/article
dc.date.updated 2018-10-03T15:05:24Z
dc.identifier.doi https://doi.org/10.1371/journal.pgen.1007563
dc.identifier.idgrec 127190

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