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Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy

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Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy

dc.contributor.author	Patwary, N.
dc.contributor.author	Sola Pikabea, Jorge
dc.contributor.author	Doblas, A.
dc.contributor.author	Saavedra Tortosa, Genaro
dc.contributor.author	Martínez Corral, Manuel
dc.contributor.author	Preza, C.
dc.date.accessioned	2018-05-02T12:51:00Z
dc.date.available	2018-05-02T12:51:00Z
dc.date.issued	2018
dc.identifier.uri	http://hdl.handle.net/10550/65969
dc.description.abstract	Three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.
dc.language.iso	eng
dc.relation.ispartof	Proceedings of the SPIE, 2018, vol. 10499, p. 1-8
dc.rights.uri	info:eu-repo/semantics/openAccess
dc.source	Patwary, N. Sola Pikabea, Jorge Doblas, A. Saavedra, G. Martínez Corral, Manuel Preza, C. 2018 Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy Proceedings of the SPIE 10499 1 8
dc.subject	Òptica Aparells i instruments
dc.title	Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy
dc.type	info:eu-repo/semantics/article
dc.date.updated	2018-05-02T12:51:01Z
dc.identifier.doi	https://doi.org/10.1117/12.2291090
dc.identifier.idgrec	124412