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Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy

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	Patwary, N.; Sola Pikabea, Jorge; Doblas, A.; Saavedra Tortosa, Genaro; Martínez Corral, Manuel Perfil; Preza, C.
	This document is a artículoDate2018
	Este documento está disponible también en : http://hdl.handle.net/10550/65969
	Three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.
	Patwary, N. Sola Pikabea, Jorge Doblas, A. Saavedra, G. Martínez Corral, Manuel Preza, C. 2018 Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured-illumination microscopy Proceedings of the SPIE 10499 1 8
	https://doi.org/10.1117/12.2291090