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Human peroxin PEX3 is co-translationally integrated into the ER and exits the ER in budding vesicles

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Human peroxin PEX3 is co-translationally integrated into the ER and exits the ER in budding vesicles

dc.contributor.author	Mayerhofer, Peter U.
dc.contributor.author	Bañó Polo, Manuel
dc.contributor.author	Mingarro Muñoz, Ismael
dc.contributor.author	Johnson, Arthur E.
dc.date.accessioned	2018-04-12T13:46:06Z
dc.date.available	2018-04-12T13:46:06Z
dc.date.issued	2016
dc.identifier.uri	http://hdl.handle.net/10550/65612
dc.description.abstract	The long-standing paradigm that all peroxisomal proteins are imported post-translationally into pre-existing peroxisomes has been challenged by the detection of peroxisomal membrane proteins (PMPs) inside the endoplasmic reticulum (ER). In mammals, the mechanisms of ER entry and exit of PMPs are completely unknown. We show that the human PMP PEX3 inserts co-translationally into the mammalian ER via the Sec61 translocon. Photocrosslinking and fluorescence spectroscopy studies demonstrate that the N-terminal transmembrane segment (TMS) of ribosome-bound PEX3 is recognized by the signal recognition particle (SRP). Binding to SRP is a prerequisite for targeting of the PEX3-containing ribosome¿nascent chain complex (RNC) to the translocon, where an ordered multistep pathway integrates the nascent chain into the membrane adjacent to translocon proteins Sec61α and TRAM. This insertion of PEX3 into the ER is physiologically relevant because PEX3 then exits the ER via budding vesicles in an ATP-dependent process. This study identifies early steps in human peroxisomal biogenesis by demonstrating sequential stages of PMP passage through the mammalian ER.
dc.language.iso	eng
dc.relation.ispartof	Traffic, 2016, vol. 17, num. 2, p. 117-130
dc.source	Mayerhofer, Peter U. Bañó Polo, Manuel Mingarro Muñoz, Ismael Johnson, Arthur E. 2016 Human peroxin PEX3 is co-translationally integrated into the ER and exits the ER in budding vesicles Traffic 17 2 117 130
dc.subject	Cèl·lules eucariotes
dc.subject	Proteïnes de membrana
dc.title	Human peroxin PEX3 is co-translationally integrated into the ER and exits the ER in budding vesicles
dc.type	journal article	es_ES
dc.date.updated	2018-04-12T13:46:06Z
dc.identifier.doi	https://doi.org/10.1111/tra.12350
dc.identifier.idgrec	108157
dc.rights.accessRights	open access	es_ES