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Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma : an in vitro study

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Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma : an in vitro study

dc.contributor.author	Ren, Guoxin	es
dc.contributor.author	Jv, Houyu	es
dc.contributor.author	Tian, Zhuowei	es
dc.contributor.author	Gvetadze, Shalva R.	es
dc.contributor.author	Hu, Jingzhou	es
dc.contributor.author	Zhao, Mei	es
dc.contributor.author	Zhou, Ge	es
dc.contributor.author	Zhang, Chenping	es
dc.contributor.author	Zhang, Zhiyuan	es
dc.date.accessioned	2017-07-24T09:29:04Z
dc.date.available	2017-07-24T09:29:04Z
dc.date.issued	2017	es
dc.identifier.uri	http://hdl.handle.net/10550/59843
dc.description.abstract	Hyperthermia is considered an efficient complement in the treatment of head and neck squamous cell carcinoma (HNSCC). Hyperthermia induces cell apoptosis in a temperature- and time-dependent manner. However, the molecular mechanism of hyperthermia remains unclear. The aim of this study was to investigate the mechanism of apoptosis induced by ultrasound hyperthermia in HNSCC cell lines HN-30 and HN-13. We examined the dynamic changes of early apoptosis and secondary necrosis in HN-30 and HN-13 cells treated by hyperthermia at 42°C for 10 min. We further examined mitochondrial membrane potential in vitro by ultrasound hyperthermia for different heating temperatures (38-44°C, 10 min) and heating times (42°C, 10-50 min). After heating by ultrasound at 42°C for 10 min, the apoptosis index achieved its highest level at 8 h after treatment, decreased rapidly and remained constant at a reduced level at 12 h. The level of secondary necrosis increased with the level of early apoptosis but remained at a higher level until 14 h. The level of secondary necrosis correlated with the level of early apoptosis (HN-13: r=0.7523, P=0.0030; HN-30: r=0.6510, P=0.016). The fractions of cells with low mitochondrial membrane potential (??) in the heating-temperature grads group and heating-time grads group decreased significantly over time. Therefore, HN-30 and HN-13 cells developed apoptosis after ultrasound hyperthermia treatment with decreases in the mitochondrial transmembrane potential level. Ultrasound hyperthermia induces apoptosis in HN-30 and HN-13 cells, possibly via the mitochondrial caspase pathway.	es
dc.source	Ren, Guoxin ; Jv, Houyu ; Tian, Zhuowei ; Gvetadze, Shalva R. ; Hu, Jingzhou ; Zhao, Mei ; Zhou, Ge ; Zhang, Chenping ; Zhang, Zhiyuan. Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma : an in vitro study. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 22 3 2017: 6-	es
dc.title	Ultrasound hyperthermia induces apoptosis in head and neck squamous cell carcinoma : an in vitro study	es
dc.type	info:eu-repo/semantics/article	en
dc.type	info:eu-repo/semantics/publishedVersion	en
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.identifier.doi	10.4317/medoral.21245	es