Citotoxicity evaluation of three dental adhesives on vero cells in vitro
NAGIOS: RODERIC FUNCIONANDO

Citotoxicity evaluation of three dental adhesives on vero cells in vitro

DSpace Repository

Citotoxicity evaluation of three dental adhesives on vero cells in vitro

Show simple item record

dc.contributor.author Catunda, Raisa Queiroz es
dc.contributor.author Vieira, Jeymesson Raphael Cardoso es
dc.contributor.author de Oliveira, Erwelly-Barros es
dc.contributor.author da Silva, Eliete Cavalcanti es
dc.contributor.author Brasil, Veruska Lima Moura es
dc.contributor.author Perez, Danyel Elias da Cruz es
dc.date.accessioned 2017-05-10T11:16:37Z
dc.date.available 2017-05-10T11:16:37Z
dc.date.issued 2017 es
dc.identifier.uri http://hdl.handle.net/10550/58448
dc.source Catunda, Raisa Queiroz ; Vieira, Jeymesson Raphael Cardoso ; de Oliveira, Erwelly-Barros ; da Silva, Eliete Cavalcanti ; Brasil, Veruska Lima Moura ; Perez, Danyel Elias da Cruz. Citotoxicity evaluation of three dental adhesives on vero cells in vitro. En: Journal of Clinical and Experimental Dentistry. 2017. Vol. 9, no. 1: 61 es
dc.subject Odontología es
dc.subject Ciencias de la salud es
dc.title Citotoxicity evaluation of three dental adhesives on vero cells in vitro es
dc.type info:eu-repo/semantics/article en
dc.type info:eu-repo/semantics/publishedVersion en
dc.subject.unesco UNESCO::CIENCIAS MÃ DICAS es
dc.description.abstractenglish To evaluate, in vitro, the potential cytotoxicity of three different dental adhesives systems (Adper Single Bond 2 -SB, Silorane System Adhesive Bond -SSAB and Single Bond Universal -SBU) on cultivated Vero cells after different contact times. The cells were cultured in a concentration of 2 x 105 cells/mL for 24h and grown to sub-confluent monolayers. VERO cells were exposed to 25µl of conditioned extracts obtained from 24h, 48h and 72h immersion of adhesive samples in culture medium (DMEM), immediately after polymerization. Fresh DMEM was used as negative control. Cell metabolism was evaluated by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5diphenyl-tetrazolium bromide). The data were analyzed statistically by ANOVA, considering a significance of 5%. The values of cell viability ranged from 94.2% at 72h (SBU) to 109.6% at 48h (SB). The mean percentage of viability after exposure to the extracts of SB, SSAB and SBU were 103.2%, 100.63% and 97.43%, respectively. There was no statistically significant difference (p= 0.342) between the experimental and negative control groups. At all exposure times, all adhesives tested in this study presented no cytotoxicity to Vero cells in vitro. es

View       (657.8Kb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics