Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)
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Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)

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Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)

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dc.contributor.author Tamborero Capilla, Silvia
dc.contributor.author Vilar, Marçal
dc.contributor.author Martínez Gil, Luis
dc.contributor.author Johnson, Arthur E.
dc.contributor.author Mingarro Muñoz, Ismael
dc.date.accessioned 2016-11-29T15:40:02Z
dc.date.available 2016-11-29T15:40:02Z
dc.date.issued 2011
dc.identifier.uri http://hdl.handle.net/10550/56203
dc.description.abstract The translocating chain-associating membrane protein (TRAM) is a glycoprotein involved in the translocation of secreted proteins into the endoplasmic reticulum (ER) lumen and in the insertion of integral membrane proteins into the lipid bilayer. As a major step toward elucidating the structure of the functional ER translocation/insertion machinery, we have characterized the membrane integration mechanism and the transmembrane topology of TRAM using two approaches: photocross-linking and truncated C-terminal reporter tag fusions. Our data indicate that TRAM is recognized by the signal recognition particle and translocon components, and suggest a membrane topology with eight transmembrane segments, including several poorly hydrophobic segments. Furthermore, we studied the membrane insertion capacity of these poorly hydrophobic segments into the ER membrane by themselves. Finally, we confirmed the main features of the proposed membrane topology in mammalian cells expressing full-length TRAM.
dc.language.iso eng
dc.relation.ispartof Journal of Molecular Biology, 2011, vol. 406, num. 4, p. 571-582
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Tamborero Capilla, Silvia Vilar, Marçal Martínez Gil, Luis Johnson, Arthur E. Mingarro Muñoz, Ismael 2011 Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM) Journal of Molecular Biology 406 4 571 582
dc.subject Proteïnes de membrana
dc.subject Membranes (Biologia)
dc.title Membrane insertion and topology of the TRanslocating chain-Associating Membrane protein (TRAM)
dc.type info:eu-repo/semantics/article
dc.date.updated 2016-11-29T15:40:03Z
dc.identifier.doi http://dx.doi.org/10.1016/j.jmb.2011.01.009
dc.identifier.idgrec 067268

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