Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets.
NAGIOS: RODERIC FUNCIONANDO

Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets.

DSpace Repository

Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets.

Show simple item record

dc.contributor.author Carda Diéguez, Miguel
dc.contributor.author Mira, Alex
dc.contributor.author Fouz Rodríguez, Belén
dc.date.accessioned 2015-07-03T08:13:08Z
dc.date.available 2015-07-03T08:13:08Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10550/45021
dc.description.abstract The routine use of chemotherapy to control bacterial diseases in aquatic populations has resulted in the development and spread of antibiotic resistance. The inclusion of immunostimulants in fish diets (functional diets) is one of the main strategies to solve this threat. This study aimed to analyse the intestinal microbiota of cultured European sea bass (Dicentrarchus labrax) fed two functional diets applying pyrosequencing of PCR-amplified 16S rRNA gene. Quality-filtered reads were assigned to family and genus taxonomic levels using the Ribosomal Database Project classifier. The autochthonous intestinal microbiota of sea bass consisted of two dominant bacterial genera: Dysgonomonas (Bacteroidetes) and Ralstonia (Betaproteobacteria), but effects of diet on this dominance were observed. In fact, the genus Dysgonomonas significantly decreased in samples from fish fed functional diets, recovering control levels at the end of the study. However, Ralstonia proportion significantly raised in samples from fish fed diet C and maintained this high level along the study period. The developed protocol could be used to study the composition of bacterial communities in the fish intestine under different nutritional and environmental conditions and its impact on infection, immune system and general fitness of fish.
dc.language.iso eng
dc.relation.ispartof Fems Microbiology Ecology, 2014, vol. 87, num. 2, p. 451-459
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Carda Diéguez, Miguel Mira, Alex Fouz Rodríguez, Belén 2014 Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets. Fems Microbiology Ecology 87 2 451 459
dc.subject Peixos
dc.subject Bacteris patògens
dc.subject Microbiologia
dc.subject Ecologia aquàtica
dc.title Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets.
dc.type info:eu-repo/semantics/article
dc.date.updated 2015-07-03T08:13:08Z
dc.identifier.doi http://dx.doi.org/10.1111/1574-6941.12236
dc.identifier.idgrec 093584

View       (1.132Mb)

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search

Browse

Statistics