Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines
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Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines

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Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines

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dc.contributor.author Ortiz, Cristian M es_ES
dc.contributor.author Ito, Tetsuo es_ES
dc.contributor.author Hashimoto, Yosuke es_ES
dc.contributor.author Nagayama, Satoshi es_ES
dc.contributor.author Iwai, Akira es_ES
dc.contributor.author Tsunoda, Shigeru es_ES
dc.contributor.author Sato, Fumiaki es_ES
dc.contributor.author Martorell, Miguel es_ES
dc.contributor.author Garcia, Jose Angel es_ES
dc.contributor.author Perez, Ana es_ES
dc.contributor.author Shimada, Yutaka es_ES
dc.date.accessioned 2015-06-29T10:32:27Z
dc.date.available 2015-06-29T10:32:27Z
dc.date.issued 2010 es_ES
dc.identifier.uri http://hdl.handle.net/10550/44796
dc.description.abstract BackgroundFascin induces membrane protrusions and cell motility. Fascin overexpression was associated with poor prognosis, and its downregulation reduces cell motility and invasiveness in esophageal squamous cell carcinoma (ESCC). Using a stable knockdown cell line, we revealed the effect of fascin on cell growth, cell adhesion and tumor formation.MethodsWe examined whether fascin is a potential target in ESCC using in vitro and in vivo studies utilizing a specific siRNA. We established a stable transfectant with downregulated fascin from KYSE170 cell line.ResultsThe fascin downregulated cell lines showed a slower growth pattern by 40.3% (p < 0.01) and detachment from collagen-coated plates by 53.6% (p < 0.01), compared to mock cells, suggesting that fascin plays a role in cell growth by maintaining cell adhesion to the extracellular matrix. In vivo, the tumor size was significantly smaller in the tumor with fascin knockdown cells than in mock cells by 95% at 30 days after inoculation.ConclusionsThese findings suggest that fascin overexpression plays a role in tumor growth and progression in ESCC and that cell death caused by its downregulation might be induced by cell adhesion loss. This indicates that targeting fascin pathway could be a novel therapeutic strategy for the human ESCC. es_ES
dc.source Diagnostic Pathology Vol. 5 pp. 41-41 es_ES
dc.title Effects of small interfering RNAs targeting fascin on human esophageal squamous cell carcinoma cell lines es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.identifier.doi 10.1186/1746-1596-5-41 es_ES
dc.identifier.idgrec 063073 es_ES

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