Functional Genomics of 5- to 8-Cell Stage Human Embryos by Blastomere Single-Cell cDNA Analysis
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Functional Genomics of 5- to 8-Cell Stage Human Embryos by Blastomere Single-Cell cDNA Analysis

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Functional Genomics of 5- to 8-Cell Stage Human Embryos by Blastomere Single-Cell cDNA Analysis

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dc.contributor.author Galán, Amparo es_ES
dc.contributor.author Montaner González, David es_ES
dc.contributor.author Póo, M. Eugenia es_ES
dc.contributor.author Valbuena, Diana es_ES
dc.contributor.author Ruiz, Verónica es_ES
dc.contributor.author Aguilar, Cristóbal es_ES
dc.contributor.author Dopazo Blázquez, Joaquín es_ES
dc.contributor.author Simón Vallés, Carlos es_ES
dc.date.accessioned 2015-06-29T10:32:27Z
dc.date.available 2015-06-29T10:32:27Z
dc.date.issued 2010 es_ES
dc.identifier.uri http://hdl.handle.net/10550/44795
dc.description.abstract Blastomere fate and embryonic genome activation (EGA) during human embryonic development are unsolved areas of high scientific and clinical interest. Forty-nine blastomeres from 5- to 8-cell human embryos have been investigated following an efficient single-cell cDNA amplification protocol to provide a template for high-density microarray analysis. The previously described markers, characteristic of Inner Cell Mass (ICM) (n = 120), stemness (n = 190) and Trophectoderm (TE) (n = 45), were analyzed, and a housekeeping pattern of 46 genes was established. All the human blastomeres from the 5- to 8-cell stage embryo displayed a common gene expression pattern corresponding to ICM markers (e.g., DDX3, FOXD3, LEFTY1, MYC, NANOG, POU5F1), stemness (e.g., POU5F1, DNMT3B, GABRB3, SOX2, ZFP42, TERT), and TE markers (e.g., GATA6, EOMES, CDX2, LHCGR). The EGA profile was also investigated between the 5-6- and 8-cell stage embryos, and compared to the blastocyst stage. Known genes (n = 92) such as depleted maternal transcripts (e.g., CCNA1, CCNB1, DPPA2) and embryo-specific activation (e.g., POU5F1, CDH1, DPPA4), as well as novel genes, were confirmed. In summary, the global single-cell cDNA amplification microarray analysis of the 5- to 8-cell stage human embryos reveals that blastomere fate is not committed to ICM or TE. Finally, new EGA features in human embryogenesis are presented. es_ES
dc.source PLoS ONE Vol. 5 Issue 10: es_ES
dc.title Functional Genomics of 5- to 8-Cell Stage Human Embryos by Blastomere Single-Cell cDNA Analysis es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.identifier.doi 10.1371/journal.pone.0013615 es_ES
dc.identifier.idgrec 062099 es_ES

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