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Comparative Genomics of Serratia spp.: Two Paths towards Endosymbiotic Life

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Comparative Genomics of Serratia spp.: Two Paths towards Endosymbiotic Life

dc.contributor.author	Manzano Marín, Alejandro	es_ES
dc.contributor.author	Lamelas, Araceli	es_ES
dc.contributor.author	Moya, Andrés	es_ES
dc.contributor.author	Latorre, Amparo	es_ES
dc.date.accessioned	2015-06-19T10:18:00Z
dc.date.available	2015-06-19T10:18:00Z
dc.date.issued	2012	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44543
dc.description.abstract	Symbiosis is a widespread phenomenon in nature, in which insects show a great number of these associations. Buchnera aphidicola, the obligate endosymbiont of aphids, coexists in some species with another intracellular bacterium, Serratia symbiotica. Of particular interest is the case of the cedar aphid Cinara cedri, where B. aphidicola BCc and S. symbiotica SCc need each other to fulfil their symbiotic role with the insect. Moreover, various features seem to indicate that S. symbiotica SCc is closer to an obligate endosymbiont than to other facultative S. symbiotica, such as the one described for the aphid Acirthosyphon pisum (S. symbiotica SAp). This work is based on the comparative genomics of five strains of Serratia, three free-living and two endosymbiotic ones (one facultative and one obligate) which should allow us to dissect the genome reduction taking place in the adaptive process to an intracellular life-style. Using a pan-genome approach, we have identified shared and strain-specific genes from both endosymbiotic strains and gained insight into the different genetic reduction both S. symbiotica have undergone. We have identified both retained and reduced functional categories in S. symbiotica compared to the Free-Living Serratia (FLS) that seem to be related with its endosymbiotic role in their specific host-symbiont systems. By means of a phylogenomic reconstruction we have solved the position of both endosymbionts with confidence, established the probable insect-pathogen origin of the symbiotic clade as well as the high amino-acid substitution rate in S. symbiotica SCc. Finally, we were able to quantify the minimal number of rearrangements suffered in the endosymbiotic lineages and reconstruct a minimal rearrangement phylogeny. All these findings provide important evidence for the existence of at least two distinctive S. symbiotica lineages that are characterized by different rearrangements, gene content, genome size and branch lengths.	es_ES
dc.source	PLoS ONE Vol. 7 Issue 10:	es_ES
dc.title	Comparative Genomics of Serratia spp.: Two Paths towards Endosymbiotic Life	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.identifier.doi	10.1371/journal.pone.0047274	es_ES
dc.identifier.idgrec	080511	es_ES