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False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3

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False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3

dc.contributor.author	Mir, Pere	es_ES
dc.contributor.author	Rodrigo Vivó, Lorena	es_ES
dc.contributor.author	Mercader, Amparo	es_ES
dc.contributor.author	Buendía Segura, Maria del Pilar	es_ES
dc.contributor.author	Mateu, Emilia	es_ES
dc.contributor.author	Milán-Sánchez, Miguel	es_ES
dc.contributor.author	Peinado Cervera, Vanessa	es_ES
dc.contributor.author	Pellicer Martínez, Antonio	es_ES
dc.contributor.author	Remohí Giménez, José	es_ES
dc.contributor.author	Simón Vallés, Carlos	es_ES
dc.contributor.author	Rubio Lluesa, Carmen	es_ES
dc.date.accessioned	2015-06-18T12:27:02Z
dc.date.available	2015-06-18T12:27:02Z
dc.date.issued	2013	es_ES
dc.identifier.uri	http://hdl.handle.net/10550/44462
dc.description.abstract	In this work, false positive rate of an arrayCGH platform for its use in day-3 single-blastomere analysis was calculated. For this purpose, 38 embryos diagnosed as abnormal on day-3 by FISH were re-biopsied on day-4. Single-cell day-4 arrayCGH diagnosis was then performed. A successful amplification was obtained in 97.4 % (37/38) of the day-4 cells analysed by arrayCGH. Day-3 FISH and day-4 arrayCGH diagnosis were concordant in 35/37 cases. The two discordant embryos were spread and all the cells from each embryo were re-analysed by FISH on day 5. The same error rate (2.7 %) for day-3 FISH and day-4 arrayCGH was obtained when comparing day-5 FISH re-analysis. After this pre-clinical phase, the platform was used for day-3 arrayCGH clinical application in 320 patients (1,760 embryos). Day-3 amplification rate was 98.6 %. An optimal reproductive outcome was obtained when applying arrayCGH to a clinical program: clinical pregnancy rate per cycle of 38.4 % and 60.3 % per transference were obtained, with an implantation rate of 53.5 %. Overall miscarriage rate was 10.6 %. Additionally, day-5 FISH re-analysis was performed in 42 of the embryos from the clinical phase, obtaining a concordance rate of 97.6 % with day-3 arrayCGH.Electronic supplementary materialThe online version of this article (doi:10.1007/s10815-012-9918-4) contains supplementary material, which is available to authorized users.	es_ES
dc.source	Journal of Assisted Reproduction and Genetics Vol. 30 Issue 1: pp. 143-149	es_ES
dc.subject	arrayCGH	es_ES
dc.subject	Blastomere biopsy	es_ES
dc.subject	Day-3 PGS	es_ES
dc.subject	Day-5 FISH re-analysis	es_ES
dc.title	False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3	es_ES
dc.type	info:eu-repo/semantics/article	es_ES
dc.identifier.doi	10.1007/s10815-012-9918-4	es_ES
dc.identifier.idgrec	087267	es_ES