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Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers

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Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers

dc.contributor.author	López Jiménez, Lidia	es
dc.contributor.author	Arnabat Domínguez, Josep	es
dc.contributor.author	Viñas Ciordia, Miquel	es
dc.contributor.author	Vinuesa Aumedes, Teresa	es
dc.date.accessioned	2015-05-08T10:40:50Z
dc.date.available	2015-05-08T10:40:50Z
dc.date.issued	2015	es
dc.identifier.uri	http://hdl.handle.net/10550/43606
dc.relation	http://www.medicinaoral.com/pubmed/medoralv20_i1_p10.pdf	es
dc.source	López Jiménez, Lidia ; Arnabat Domínguez, Josep ; Viñas Ciordia, Miquel ; Vinuesa Aumedes, Teresa. Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers. En: Medicina oral, patología oral y cirugía bucal. Ed inglesa, 2015, Vol. 20, No. 1: 10-	es
dc.subject	Odontología	es
dc.subject	Ciencias de la salud	es
dc.title	Atomic force microscopy visualization of injuries in Enterococcus faecalis surface caused by Er,Cr:YSGG and diode lasers	es
dc.type	info:eu-repo/semantics/article	en
dc.type	info:eu-repo/semantics/publishedVersion	en
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.description.abstractenglish	Aim: To visualize by Atomic Force Microscopy the alterations induced on Enterococcus. faecalis surface after treatment with 2 types of laser: Erbium chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser and Diode laser. Material and Methods: Bacterial suspensions from overnight cultures of E. faecalis were irradiated during 30 seconds with the laser-lights at 1 W and 2 W of power, leaving one untreated sample as control. Surface alterations on treated E. faecalis were visualized by Atomic Force Microscopy (AFM) and its surface roughness determined. Results: AFM imaging showed that at high potency of laser both cell morphology and surface roughness resulted altered, and that several cell lysis signs were easily visualized. Surface roughness clearly increase after the treatment with Er,Cr:YSGG at 2W of power, while the other treatments gave similar values of surface roughness. The effect of lasers on bacterial surfaces visualized by AFM revealed drastic alterations. Conclusions: AFM is a good tool to evaluate surface injuries after laser treatment; and could constitute a measure of antimicrobial effect that can complete data obtained by determination of microbial viability.	es