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pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

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pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance

dc.contributor.author	Amaro González, Carmen
dc.contributor.author	Baker-Austin, Craig
dc.contributor.author	Lemm, Elizabeth
dc.contributor.author	Hartnell, Rachel
dc.contributor.author	Lowther, James
dc.contributor.author	Onley, Richard
dc.contributor.author	Oliver, James D.
dc.contributor.author	Lees, David
dc.date.accessioned	2014-02-26T09:48:45Z
dc.date.available	2014-02-26T09:48:45Z
dc.date.issued	2012
dc.identifier.uri	http://dx.doi.org/10.1016/j.fm.2011.09.002
dc.identifier.uri	http://hdl.handle.net/10550/33333
dc.description.abstract	The Gram-negative bacterium Vibrio vulnificus is a common inhabitant of estuarine environments. Globally, V. vulnificus is a significant foodborne pathogen capable of causing necrotizing wound infections and primary septicemia, and is a leading cause of seafood-related mortality. Unfortunately, molecular methods for the detection and enumeration of pathogenic V. vulnificus are hampered by the genetically diverse nature of this pathogen, the range of different biotypes capable of infecting humans and aquatic animals, and the fact that V. vulnificus contains pathogenic as well as non-pathogenic variants. Here we report an alternative approach utilizing the development of a real-time PCR assay for the detection of pathogenic V. vulnificus strains based on a polymorphism in pilF, a gene previously indicated to be associated with human pathogenicity. Compared to human serum reactivity, the real-time PCR assay successfully detected pathogenic strains in 46 out of 47 analysed V. vulnificus isolates (97.9%). The method is also rapid, sensitive, and more importantly can be reliably utilised on biotype 2 and 3 strains, unlike other current methods for V. vulnificus virulence differentiation.
dc.relation.ispartof	Food Microbiology, 2012, vol. 30, num. 1, p. 17-23
dc.rights.uri	info:eu-repo/semantics/openAccess
dc.source	Amaro González, Carmen Baker-Austin, Craig Lemm, Elizabeth Hartnell, Rachel Lowther, James Onley, Richard Oliver, James D. Lees, David 2012 pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance Food Microbiology 30 1 17 23
dc.subject	Microbiologia
dc.title	pilF polymorphism-based real-time PCR to distinguish Vibrio vulnificus strains of human health relevance
dc.type	info:eu-repo/semantics/article
dc.date.updated	2014-02-26T09:48:45Z
dc.identifier.doi	http://dx.doi.org/10.1016/j.fm.2011.09.002
dc.identifier.idgrec	075974