Regulon-specific control of transcription elongation across the yeast genome
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Regulon-specific control of transcription elongation across the yeast genome

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Regulon-specific control of transcription elongation across the yeast genome

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dc.contributor.author Pelechano García, Vicente José
dc.contributor.author Jimeno-González, S.
dc.contributor.author Rodríguez-Gil, Alfonso
dc.contributor.author García Martínez, José
dc.contributor.author Pérez Ortín, José Enrique
dc.contributor.author Chávez, Sebastián
dc.date.accessioned 2013-12-04T13:49:52Z
dc.date.available 2013-12-04T13:49:52Z
dc.date.issued 2009
dc.identifier.uri http://dx.doi.org/10.1371/journal.pgen.1000614
dc.identifier.uri http://hdl.handle.net/10550/32053
dc.description.abstract Transcription elongation by RNA polymerase II was often considered an invariant non-regulated process. However, genome-wide studies have shown that transcriptional pausing during elongation is a frequent phenomenon in tightly-regulated metazoan genes. Using a combination of ChIP-on-chip and genomic run-on approaches, we found that the proportion of transcriptionally active RNA polymerase II (active versus total) present throughout the yeast genome is characteristic of some functional gene classes, like those related to ribosomes and mitochondria. This proportion also responds to regulatory stimuli mediated by protein kinase A and, in relation to cytosolic ribosomal-protein genes, it is mediated by the silencing domain of Rap1. We found that this inactive form of RNA polymerase II, which accumulates along the full length of ribosomal protein genes, is phosphorylated in the Ser5 residue of the CTD, but is hypophosphorylated in Ser2. Using the same experimental approach, we show that the in vivo<br>depletion of FACT, a chromatin-related elongation factor, also produces a regulon-specific effect on the expression of the yeast genome. This work demonstrates that the regulation of transcription elongation is a widespread, gene class<br>dependent phenomenon that also affects housekeeping genes.
dc.relation.ispartof Plos Genetics, 2009, vol. 5, num. 8, p. e1000614
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Pelechano García, Vicente José Jimeno-González, S. Rodríguez-Gil, Alfonso García Martínez, José Pérez Ortín, José Enrique Chávez, Sebastián 2009 Regulon-specific control of transcription elongation across the yeast genome Plos Genetics 5 8 e1000614
dc.subject RNA
dc.subject Transcripció genètica
dc.subject Genètica molecular
dc.subject Saccharomyces
dc.title Regulon-specific control of transcription elongation across the yeast genome
dc.type info:eu-repo/semantics/article
dc.date.updated 2013-12-04T13:49:52Z
dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1000614
dc.identifier.idgrec 052954

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