There is a steady-state transcriptome in exponentially growing yeast cells
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There is a steady-state transcriptome in exponentially growing yeast cells

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There is a steady-state transcriptome in exponentially growing yeast cells

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dc.contributor.author Pelechano García, Vicente José
dc.contributor.author Pérez Ortín, José Enrique
dc.date.accessioned 2013-12-04T13:37:33Z
dc.date.available 2013-12-04T13:37:33Z
dc.date.issued 2010
dc.identifier.uri http://dx.doi.org/10.1002/yea.1768
dc.identifier.uri http://hdl.handle.net/10550/32050
dc.description.abstract The growth of yeast cells in batches in glucose-based media is a standard condition in most yeast laboratories. Most gene expression experiments are done by taking this condition as a reference. Presumably, cells are in a stable physiological condition that can be easily reproduced in other laboratories. With this assumption, however, it is necessary to consider that the average amount of the mRNAs per cell for most genes does not change during exponential growth. That is to say, there is a steady-state condition for the transcriptome. However, this has not been rigorously demonstrated to date. In this work we take several cell samples during the exponential phase growth to perform a kinetic study using the genomic run-on (GRO) technique, which allows simultaneous measurement of the amount of mRNA and transcription rate variation at the genomic level. We show here that the steady-state condition is fulfilled for almost all the genes during most exponential growth in yeast extract<br>peptone<br>dextrose medium (YPD) and, therefore, that simultaneous measures of the transcription rates and the amounts of mRNA can be used for indirect mRNA stability calculations. With this kinetic approach, we were also able to dermine the relative influence of the transcription rate and the mRNA stability changes for the mRNA variation for those genes that deviate from the steady state.
dc.relation.ispartof Yeast, 2010, vol. 27, num. 7, p. 413-422
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Pelechano García, Vicente José. Pérez Ortín, José Enrique 2010 There is a steady-state transcriptome in exponentially growing yeast cells Yeast 27 7 413 422
dc.subject Genètica molecular
dc.subject RNA
dc.subject Transcripció genètica
dc.subject Saccharomyces
dc.title There is a steady-state transcriptome in exponentially growing yeast cells
dc.type info:eu-repo/semantics/article
dc.date.updated 2013-12-04T13:37:33Z
dc.identifier.doi http://dx.doi.org/10.1002/yea.1768
dc.identifier.idgrec 060542

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