A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding
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A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding

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A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding

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dc.contributor.author Bermúdez, Ignacio
dc.contributor.author García Martínez, José
dc.contributor.author Pérez Ortín, José Enrique
dc.contributor.author Roca, Joaquim
dc.date.accessioned 2013-12-04T12:31:27Z
dc.date.available 2013-12-04T12:31:27Z
dc.date.issued 2010
dc.identifier.uri http://dx.doi.org/10.1093/nar/gkq687
dc.identifier.uri http://hdl.handle.net/10550/32047
dc.description.abstract The helical tension of chromosomal DNA is one of the epigenetic landmarks most difficult to examine experimentally. The occurrence of DNA crosslinks mediated by psoralen photobinding (PB) stands as the only suitable probe for assessing this problem. PB is affected by chromatin structure when is done to saturation; but it is mainly determined by DNA helical tension when it is done to very low hit conditions. Hence, we developed a method for genomewide analysis of DNA helical tension based on PB. We adjusted in vitro PB conditions that discern DNA helical tension and applied them to Saccharomyces cerevisiae cells. We selected the in vivo cross-linked DNA sequences and identified them on DNA arrays. The entire procedure was robust. Comparison of PB obtained in vivo with that obtained in vitro with naked DNA revealed that numerous chromosomal regions had deviated PB values. Similar analyses in yeast topoisomerase mutants uncovered further PB alterations across specific chromosomal domains. These results suggest that distinct chromosome compartments might confine different levels of DNA helical tension in yeast. Genome-wide analysis of psoralen<br>DNA PB can be, therefore, a useful approach to uncover a trait of the chromosome architecture not amenable to other techniques.
dc.relation.ispartof Nucleic Acids Research, 2010, vol. 38, num. 19, p. e182
dc.rights.uri info:eu-repo/semantics/openAccess
dc.source Bermúdez, Ignacio García Martínez, José Pérez Ortín, José Enrique Roca, Joaquim 2010 A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding Nucleic Acids Research 38 19 e182
dc.subject Genòmica
dc.subject Genètica molecular
dc.subject ADN
dc.title A method for genome-wide analysis of DNA helical tension by means of psoralen-DNA photobinding
dc.type info:eu-repo/semantics/article
dc.date.updated 2013-12-04T12:31:27Z
dc.identifier.doi http://dx.doi.org/10.1093/nar/gkq687
dc.identifier.idgrec 060544

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