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Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context

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Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context

dc.contributor.author	Comas Espadas, Iñaki
dc.contributor.author	González Candelas, Fernando
dc.contributor.author	Zúñiga, Manuel
dc.date.accessioned	2010-07-13T08:11:22Z
dc.date.available	2010-07-13T08:11:22Z
dc.date.issued	2008
dc.identifier.uri	http://hdl.handle.net/10550/15997
dc.description.abstract	Background The phosphoenolpyruvate phosphotransferase system (PTS) plays a major role in sugar transport and in the regulation of essential physiological processes in many bacteria. The PTS couples solute transport to its phosphorylation at the expense of phosphoenolpyruvate (PEP) and it consists of general cytoplasmic phosphoryl transfer proteins and specific enzyme II complexes which catalyze the uptake and phosphorylation of solutes. Previous studies have suggested that the evolution of the constituents of the enzyme II complexes has been driven largely by horizontal gene transfer whereas vertical inheritance has been prevalent in the general phosphoryl transfer proteins in some bacterial groups. The aim of this work is to test this hypothesis by studying the evolution of the phosphoryl transfer proteins of the PTS. Results We have analyzed the evolutionary history of the PTS phosphoryl transfer chain (PTS-ptc) components in 222 complete genomes by combining phylogenetic methods and analysis of genomic context. Phylogenetic analyses alone were not conclusive for the deepest nodes but when complemented with analyses of genomic context and functional information, the main evolutionary trends of this system could be depicted. Conclusion The PTS-ptc evolved in bacteria after the divergence of early lineages such as Aquificales, Thermotogales and Thermus/Deinococcus. The subsequent evolutionary history of the PTS-ptc varied in different bacterial lineages: vertical inheritance and lineage-specific gene losses mainly explain the current situation in Actinobacteria and Firmicutes whereas horizontal gene transfer (HGT) also played a major role in Proteobacteria. Most remarkably, we have identified a HGT event from Firmicutes or Fusobacteria to the last common ancestor of the Enterobacteriaceae, Pasteurellaceae, Shewanellaceae and Vibrionaceae. This transfer led to extensive changes in the metabolic and regulatory networks of these bacteria including the development of a novel carbon catabolite repression system. Hence, this example illustrates that HGT can drive major physiological modifications in bacteria.	en
dc.language.iso	en	en
dc.relation	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2405797/pdf/1471-2148-8-147.pdf	en
dc.source	Comas,I.; González-Candelas,F.; Zúñiga,M. Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context.En BMC Evolutionary Biology 2008, 8:147	en
dc.subject	Phosphoenolpyruvate phosphotransferase system (PTS); Cytoplasmic phosphoryl transfer proteins; PTS phosphoryl transfer chain (PTS-ptc)	en
dc.title	Unraveling the evolutionary history of the phosphoryl-transfer chain of the phosphoenolpyruvate:phosphotransferase system through phylogenetic analyses and genome context	en
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS DE LA VIDA::Biología celular::Citogenética	en
dc.identifier.doi	10.1186/1471-2148-8-147	en
dc.description.private	coesi@uv.es; gonzalef@uv.es	en
dc.identifier.idgrec	047604	en
dc.type.hasVersion	VoR	es_ES